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Figure 1


Fig. 1. Flotillin microdomains redistribute in response to stimulation of cells with EGF. (A) Colocalisation of endogenous flotillin-1 and flotillin-2 revealed by indirect immunofluorescence. Flotillin-1 was detected with an affinity-purified anti-flotillin-1 polyclonal antibody (Frick et al., 2007), flotillin-2 with a specific monoclonal antibody. Insets show magnified view of the boxed areas. Scale bars: 5 µm. (B) Single confocal sections of NIH3T3 cells either starved, or stimulated for 30 minutes with EGF. Flotillin-1 and flotillin-2 was detected as in A. Scale bars: 20 µm. (C) Flotillin-2 detected with a monoclonal antibody, in HeLa cells treated as shown. TIR images showing the bottom ~100 nm of cells. EGF was added for 30 minutes. Scale bars: 20 µm. (D) Quantification of the mean fluorescence intensity of flotillin-2 in TIR images of HeLa and NIH3T3 cells treated as shown. Error bars represent s.e.m.; n>10.





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