JCS040840 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Expanded view of images in Fig. 7A showing neighboring untransfected cells next to successfully transfected cells.

• Movie 1 -

  Movie 1. U2SO cell expressing YFP-PML-III. Images were captured at intervals of 1 second for a total imaging time of 2 minutes. The red line indicates the nuclear boundary. Arrows indicate cytoplasmic foci that exhibit short-burst movements.

• Movie 2 -

  Movie 2. U2OS cell expressing YFP-PML-III (shown in yellow) and mCherry (shown in gray to visualize cytoplasmic and nuclear boundaries). Z-stacks of images were collected at intervals of 30 seconds, and the total imaging time is 40 minutes.

• Movie 3 -

  Movie 3. U2OS cell expressing YFP-PML-III. Images were captured at intervals of 30 seconds, and the total imaging time is 40 minutes. The movie shows two cytoplasmic particles that become stably associated with the nuclear membrane.

• Movie 4 -

  Movie 4. Effect of nocodazole treatment. U2OS cells expressing YFP-PML-III (yellow) and mCherry (gray). The data were obtained as described for Movie 2.

• Movie 5 -

  Movie 5. U2OS cell expressing CFP-PML-III (shown in yellow) and YFP-α-tubulin (shown in blue). Images were captured at intervals of 1 second. The movie represents 43 seconds of imaging.