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First published online April 1, 2009

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Cytokinesis: p0071 finds its way

During mammalian cytokinesis, the GTPase RhoA directs the formation and contraction of a cortical actomyosin ring at the midzone of the mitotic spindle. Activation of RhoA at the midzone requires the interaction of the plakophilin p0071 and the RhoGEF Ect2 – but how is transport of the two proteins coordinated? On page 1174, Mechthild Hatzfeld and colleagues show that p0071 and Ect2 are transported to the spindle midbody by distinct mechanisms. The authors first demonstrate that the transport of Ect2 to the midbody (which is known to be mediated by the kinesin MKLP1, a component of the centralspindlin complex) occurs before the accumulation of p0071. They next show that p0071 interacts directly with the kinesin-2 family member KIF3b. Notably, a p0071 mutant that does not bind to KIF3b fails to localise at the midbody, and depletion of either KIF3b or p0071 inhibits the accumulation of actin and myosin at the midbody and decreases levels of active RhoA during cytokinesis. Finally, the authors show that a fusion protein of p0071 and the MKLP1 motor domain can rescue RhoA activation at the midbody. Thus, the authors conclude, Ect2 and p0071 are targeted to the midbody by distinct motor proteins. Their data also identify a novel pathway of KIF3b-dependent actin reorganisation in cytokinesis.

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Targeting of p0071 to the midbody depends on KIF3

René Keil, Christina Kießling, and Mechthild Hatzfeld
JCS 2009 122: 1174-1183. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?