JCS044255 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Two Coomassie-blue-stained gels to demonstrate relative amounts of GST fusion proteins used for the Ubc7p binding assay in Fig. 2B. The same amount of GST-Cue1p^25-203 was loaded in both gels; however, the two were stained at different times.

• Supplemental Figure S2 -

  Fig. S2. Long exposure of HA immunoblot from Fig. 5A bottom right panel demonstrating expression and rapid degradation of Ubc7pHA in cells expressing Cue1p^1-173.