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Figure 3


Fig. 3. Soluble Cue1p can restore in vivo ERAD and stabilize Ubc7p. (A) Cycloheximide (CHX) chase assays were performed to determine degradation of CPY* and stability of Ubc7pHA in {Delta}cue1{Delta}ubc7 expressing Ubc7pHA together with the indicated regions of Cue1p. A longer exposure of the HA immunoblot is also shown to demonstrate that Ubc7pHA is expressed under all conditions. (B) {Delta}cue1 cells were transformed with plasmids containing no CUE1 gene (V), CUE1 or cue124-203. Degradation of CPY* was assayed by cycloheximide chase analysis at the indicated times. Pgk1p was used as loading control.





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