JCS046466 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. DAPI images of WT and 34 mutants in proliferation. Scale bars: 10 µm.

• Supplemental Figure S2 -

  Fig. S2. DAPI images of WT and 34 mutants in quiescence.

• Supplemental Table S1 -

  Table S1. ≥Fourfold increased (14) or decreased (48) gene transcripts in fcp1 mutant in the +N medium.

• Supplemental Table S2 -

  Table S2. ≥Fourfold increased (142) or decreased (117) gene transcripts in fcp1 mutant in the -N medium.

• Supplemental Table S3 -

  Table S3. ≥Fourfold increased or decreased transcripts, containing the signal peptide sequence, the trans-membrane domain, fluctuating in the cell cycle (cluster 1-4), responsive to stress, coding for mitochondrial proteins and gene silencing or chromatin remodeling proteins.

• Movie 1 -

  Movie 1. S. pombe cells grown in the +N medium were shifted to the −N medium for 15 hours. Two cell divisions occur, and resulting G0 cells are round. These non-dividing cells are viable for months.

• Movie 2 -

  Movie 2. Cells of wis1-982 mutant grown in the +N medium were shifted to the −N medium for 15 hours.

• Movie 3 -

  Movie 3. Cells of sty1-989 mutant grown in the +N medium were shifted to the −N medium for 15 hours.

• Movie 4 -

  Movie 4. Cells of vam6-532 grown in the +N medium were shifted to the −N medium for 15 hours.

• Movie 5 -

  Movie 5. Cells of wsp1-318 cultured in the −N medium for 24 hours, then replenished on the rich YPD agar medium for 20 hours were taken by movie. Many cells were disrupted and shrunk.

• Movie 6 -

  Movie 6. Cells of end4-507 cultured in the −N medium for 24 hours, then replenished on the rich YPD agar medium for 20 hours were taken by movie. Many cells were disrupted and shrunk.

• Movie 7 -

  Movie 7. Cells of end4-507 cultured in the −N medium for 96 hours, then replenished on the rich YPD agar medium for 20 hours were taken by movie. Most cells started to grow at normal timing but in a mono-polar growth manner.

• Movie 8 -

  Movie 8. Cells of the wild type cultured in the −N medium for 24 hours, then replenished on the rich YPD agar medium for 20 hours were taken by movie. Cells were grown in a bi-polar manner.