JCS000703 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. (A) HEK293 cells were co-transfected with CIT-K and GFP-TTC3 or GFP-ΔCterm. Lysates from transfected cells were then immunoprecipitated with anti-Citron antibodies and samples were analyzed by western blotting as indicated. (B) CIT-K was co-transfected with TTC3 mutants (ΔCterm and ΔCBR). Lysates were immunoprecipitated with anti-GFP antibodies and samples were analyzed by western blotting as indicated.

• Supplemental Figure S2 -

  Fig. S2. PC12 cells transfected with GFP alone or with GFP-TTC3 where allowed to differentiate by NGF treatment for 3 days, and apoptotic cells were evidenced by immunofluorescence with anti-activated Caspase 3 antibodies. Round cells overexpressing TTC3 were mostly negative. The bottom panel shows a positive non-transfected control cell.

• Supplemental Figure S3 -

  Fig. S3. (A) HEK293 cells were transfected with pDECAP control (pD), pDECAP-CIT-K (pD-CIT), pSUPER control (pS) or pSUPER-CIT-K (pS-CIT), and the endogenous levels of CIT-K were evaluated by western blotting. We used as internal loading control the closely related kinase ROK-α. Bar, 10 μm. (B-C) The effects of CIT-K-RNAi in PC12 cells, were analyzed exactly as in Fig. 3. Data are means ±s.d.