JCS022103 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. mgp1⁻/2⁻ cells are unaltered in their resistance to hypo-osmotic shock. AX3 (A) and mgp1⁻/2⁻ cells (B) were subjected to hypo-osmotic shock by replacing axenic growth medium (Time 0) with distilled water (1 minute onwards). Scale bar: 10 µm.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Tubular-vesicular elements of the contractile vacuole network. The tubular-vesicular elements of the contractile vacuole network of wild type AX3 and mgp1⁻/2⁻ cells in iso-osmotic or hypo-osmotic conditions visualised with FM2-10. Scale bar: 10 µm. Pictures are representative of three individual experiments.

• Movie 1 -

  Movie 1. mgp1⁻ cells have more contractile vacuoles. Contractile vacuole activity was stimulated by incubating cells in growth medium diluted to one-third strength in distilled water. The contractile vacuole network of cells visualised with the lipophilic styryl dye FM2-10. Both mgp1⁻ and mgp1⁻/2⁻ cells show many more contractile vacuoles than the wild type.

• Movie 2 -

  Movie 2. mgp1⁻ cells expressing GFP-MEGAP1. In a hypo-osmotic environment, GFP-MEGAP1 transiently localizes to contractile vacuoles at the point of discharge.

• Movie 3 -

  Movie 3. mgp1⁻ cells expressing GFP-MEGAP1. In an iso-osmotic environment, GFP-MEGAP1 is distributed between the cytosol and the tubular network of the contractile vacuole system.