JCS02949 Supplementary Material

Files in this Data Supplement:

• Supplemental Table 1 - Adobe PDF

• Supplemental Figure 1 -

  Fig. S1. Anti T. gondii MORN1 antibody labeling in Sarcocystis neurona (A). In S. neurona spindles (red) persist during interphase and 32 are visible here on the polyploid nucleus (DAPI) (B). MORN1 staining (A) overlaps with tubulin staining (B) on the nuclear envelope. In the enlarged inset (C) MORN1 appears to localize to the center of each spindle rather than to the spindle pole extremes. (D-F) Mature S. neurona merozoites ready to egress present posterior, anterior and nuclear MORN1 structures indistinguishable from T. gondii localization (inset (F) highlights an individual merozoite).

• Movie 1 -

  Movie 1. HFF cells were infected with stable MORN1-YFP transgenic parasites and imaged after 24 hours. 200 nm Z-sections were recorded on a DeltaVision microscope and then deconvolved using SoftWorx. Two forming daughter parasites are visible within the mother parasite as rings capping the posterior end of the forming daughter IMC. The two bright spots represent the centrocones, the weakly labeled structures represent the apical end of the forming IMCs. At the left bottom corner of the image the mother’s posterior MORN1 ring is visible.

• Movie 2 -

  Movie 2. Cells were imaged as described for movie 1. A rosette of eight dividing pararasites within a single vacuole is visible. The posterior mother rings are centrally localized while the IMC capping rings and bright centrocones are visible on the outer radius The most outward weak structures are the apical ends of the forming daughter IMC.

• Movie 3 -

  Movie 3. Time-lapse movie of nucleus and MORN1 during parasite division. The nucleus is visualized in red (H2B-mRFP) and the MORN1 in green (MORN1-YFP). Frames were collected every 10 minutes for a total of 270 minutes.

• Movie 4 -

  Movie 4. 3D projection of the deconvolved stack represented in Fig. 5 J. Imaged as described for Movie 3.