Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers

QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

This Article

	Summary
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager


Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Wang, J.
	Articles by Reeves, W. H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Wang, J.
	Articles by Reeves, W. H.


Social Bookmarking

	What's this?

Allaway, G. P., Vivino, A. A., Kohn, L. D., Notkins, A. L. and Prabhakar, B. S (1989). Characterization of the 70 kDa component of Ku autoantigen expressed in insect cell nuclei using a baculovirus vector. Biochem. Biophys. Res. Commun 168, 747-755.

Anderson, C. W (1993). DNA damage and the DNA-activated protein kinase. Trends Biochem. Sci 18, 433-437.[Medline]

Blier, P. R., Griffith, A. J., Craft, J. and Hardin, J. A (1993). Binding of Ku protein to DNA. Measurement of affinity for ends and demonstration of binding to nicks. J. Biol. Chem 268, 7594-7601.[Abstract/Free Full Text]

Booher, R. N., Alfa, C. E., Hyams, J. S. and Beach, D. H (1989). The fission yeast cdc2/cdc13/suc1 protein kinase: Regulation of catalytic activity and nuclear localization. Cell 58, 485-497.[Medline]

Bravo, R. and Celis, J. E (1982). Human proteins sensitive to neoplastic transformation in cultured epithelial and fibroblast cells. Clin. Chem 28, 949-954.[Abstract]

Carter, T., Vancurova, I., Sun, I., Lou, W. and DeLeon, S (1990). A DNA-activated protein kinase from HeLa cell nuclei. Mol. Cell. Biol 10, 6460-6471.[Abstract/Free Full Text]

Celis, J. E., Madsen, P., Nielsen, S., Ratz, G. P., Lauridsen, J. B. and Celis, A (1987). Levels of synthesis of primate-specific nuclear proteins differ between growth-arrested and proliferating cells. Exp. Cell Res 168, 389-401.[Medline]

Chou, C. H., Wang, J., Knuth, M. W. and Reeves, W. H (1992). Role of a major autoepitope in forming the DNA binding site of the p70 (Ku) antigen. J. Exp. Med 175, 1677-1684.[Abstract/Free Full Text]

Dvir, A., Peterson, S. R., Knuth, M. W., Lu, H. and Dynan, W. S (1992). Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II. Proc. Nat. Acad. Sci. USA 89, 11920-11924.[Abstract/Free Full Text]

Falkner, F. G. and Moss, B (1988). Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors. J. Virol 62, 1849-1854.[Abstract/Free Full Text]

Falzon, M., Fewell, J. W. and Kuff, E. L (1993). EPB-80, a transcription factor closely resembling the human autoantigen Ku, recognizes single-to double-strand transitions in DNA. J. Biol. Chem 268, 10546-10552.[Abstract/Free Full Text]

Feldmann, H. and Winnacker, E. L (1993). A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae. J. Biol. Chem 268, 12895-12900.[Abstract/Free Full Text]

Gottlieb, T. M. and Jackson, S. P (1993). The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72, 131-142.[Medline]

Griffith, A. J., Blier, P. R., Mimori, T. and Hardin, J. A (1992). Ku polypeptides synthesized in vitro assemble into complexes which recognize ends of double-stranded DNA. J. Biol. Chem 267, 331-338.[Abstract/Free Full Text]

Higashiura, M., Shimizu, Y., Tanimoto, M., Morita, T. and Yagura, T (1992). Immunolocalization of Ku-proteins (p80/p70): localization of p70 to nucleoli and periphery of bith interphase nuclei and metaphase chromosomes. Exp. Cell Res 201, 444-451.[Medline]

Hoff, C. M. and Jacob, S. T (1993). Characterization of the factor E1BF from a rat hepatoma that modulates ribosomal RNA gene transcription and its relationship to the human Ku autoantigen. Biochem. Biophys. Res. Commun 190, 747-753.[Medline]

Hong, J. S. and Engler, J. A (1991). The amino terminus of the adenovirus fiber protein encodes the nuclear localization signal. Virology 185, 758-767.[Medline]

Kaczmarski, W. and Khan, S. A (1993). Lupus autoantigen Ku protein binds HIV-1 TAR RNA in vitro. Biochem. Biophys. Res. Commun 196, 935-942.[Medline]

Knuth, M. W., Gunderson, S. I., Thompson, N. E., Strasheim, L. A. and Burgess, R. R (1990). Purification and characterization of PSE1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J. Biol. Chem 265, 17911-17920.[Abstract/Free Full Text]

Lees-Miller, S. P., Chen, Y. R. and Anderson, C. W (1990). Human cells contain a DNA-activated protein kinase that phosphorylates simian virus 40 T antigen, mouse p53, and the human Ku autoantigen. Mol. Cell. Biol 10, 6472-6481.[Abstract/Free Full Text]

Lees-Miller, S., Sakaguchi, K., Ullrich, S. J., Appella, E. and Anderson, C. W (1992). Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53. Mol. Cell. Biol 12, 5041-5049.[Abstract/Free Full Text]

Li, L. L. and Yeh, N. H (1992). Cell cycle-dependent migration of the DNA-binding protein Ku80 into nucleoli. Exp. Cell Res 199, 262-268.[Medline]

May, G., Sutton, C. and Gould, H (1991). Purification and characterization of Ku-2, an octamer-binding protein related to the autoantigen Ku. J. Biol. Chem 266, 3052-3059.[Abstract/Free Full Text]

Mimori, T., Akizuki, M., Yamagata, H., Inada, S., Yoshida, S. and Homma, M (1981). Characterization of a high molecular weight acidic nuclear protein recognized by autoantibodies from patients with polymyositis-scleroderma overlap. J. Clin. Invest 68, 611-620.

Mimori, T. and Hardin, J. A (1986). Mechanism of interaction between Ku protein and DNA. J. Biol. Chem 261, 10375-10379.[Abstract/Free Full Text]

Nuesch, J. P. and Tattersall, P (1993). Nuclear targeting of the parvoviral replicator molecule NS1: evidence for self-association prior to nuclear transport. Virology 196, 637-651.[Medline]

Reeves, W. H (1985). Use of monoclonal antibodies for the characterization of novel DNA-binding proteins recognized by human autoimmune sera. J. Exp. Med 161, 18-39.[Abstract/Free Full Text]

Reeves, W. H (1987). Antinuclear antibodies as probes to explore the structural organization of the genome. J. Rheumatol 14, 13-.

Reeves, W. H. and Sthoeger, Z. M (1989). Molecular cloning of cDNA encoding the p70 (Ku) lupus autoantigen. J. Biol. Chem 264, 5047-5052.[Abstract/Free Full Text]

Reeves, W. H., Pierani, A., Chou, C. H., Ng, T., Nicastri, C., Roeder, R. G. and Sthoeger, Z. M (1991). Epitopes of the p70 and p80 (Ku) lupus autoantigens. J. Immunol 146, 2678-2686.[Abstract]

Roberts, M. R., Miskimins, W. K. and Ruddle, F. H (1989). Nuclear proteins TREF1 and TREF2 bind to the transcriptional control element of the transferrin receptor gene and appear to be associated as a heterodimer. Cell Regul 1, 151-164.[Medline]

Shi, Y. and Thomas, J. O (1992). The transport of proteins into the nucleus requires the 70-kilodalton heat shock protein or its cytosolic cognate. Mol. Cell. Biol 12, 2186-2192.[Abstract/Free Full Text]

Stuiver, M. H., Coenjaerts, F. E. J. and van der Vliet, P. C (1990). The autoantigen Ku is indistinguishable from NF IV, a protein forming multimeric protein-DNA complexes. J. Exp. Med 172, 1049-1054.[Abstract/Free Full Text]

Stuiver, M. H., Celis, J. E. and van der Vliet, P. C (1991). Identification of nuclear factor IV/Ku autoantigen in a human 2D-gel protein database. FEBS Lett 282, 189-192.[Medline]

Stunnenberg, H. G., Lange, H., Philipson, L., van Miltenburg, R. T. and van der Vliet, P. C (1988). High expression of functional DNA polymerase and precursor terminal protein using recombinant vaccinia virus. Nucl. Acids Res 16, 2431-2444.[Abstract/Free Full Text]

Wang, J., Chou, C. H., Blankson, J., Satoh, M., Knuth, M. W., Eisenberg, R. A., Pisetsky, D. S. and Reeves, W. H (1993). Murine monoclonal antibodies specific for conserved and non-conserved antigenic determinants of the human and murine Ku autoantigens. Mol. Biol. Rep 18, 15-28.[Medline]

Wen, J. and Yaneva, M (1990). Mapping of epitopes on the 86 kDa subunit of the Ku autoantigen. Mol. Immunol 27, 973-980.[Medline]

Yaneva, M., Ochs, R., McRorie, D. K., Zweig, S. and Busch, H (1985). Purification of an 86-70 kDa nuclear DNA-associated protein complex. Biochim. Biophys. Acta 841, 22-29.[Medline]

Yaneva, M. and Busch, H (1986). A 10S particle released from deoxyribonuclease-sensitive regions of HeLa cell nuclei contains the 86-kilodalton-70-kilodalton protein complex. Biochemistry 25, 5057-5063.[Medline]

Yaneva, M., Wen, J., Ayala, A. and Cook, R (1989). cDNA-derived amino acid sequence of the 86-kDa subunit of the Ku antigen. J. Biol. Chem 264, 13407-13411.[Abstract/Free Full Text]

Zhao, L. J. and Padmanabhan, R (1988). Nuclear transport of adenovirus DNA polymerase is facilitated by interaction with preterminal protein. Cell 55, 1005-1015.[Medline]

Zhou, J., Doorbar, J., Sun, X. Y., Crawford, L. V., McLean, C. S. and Frazer, I. H (1991). Identification of the nuclear localization signal of human papillomavirus type 16 L1 protein. Virology 185, 625-632.[Medline]

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Technorati

Twitter What's this?

This article has been cited by other articles:

T. Mesplede, M.-L. Island, N. Christeff, F. Petek, J. Doly, and S. Navarro
The POU Transcription Factor Oct-1 Represses Virus-Induced Interferon A Gene Expression
Mol. Cell. Biol., October 1, 2005; 25(19): 8717 - 8731.
[Abstract] [Full Text] [PDF]

F. Hammer, N. A. Compagnone, J.-L. Vigne, S. R. Bair, and S. H. Mellon
Transcriptional Regulation of P450scc Gene Expression in the Embryonic Rodent Nervous System
Endocrinology, February 1, 2004; 145(2): 901 - 912.
[Abstract] [Full Text] [PDF]

A. Schaffer, E. C. Kim, X. Wu, H. Zan, L. Testoni, S. Salamon, A. Cerutti, and P. Casali
Selective Inhibition of Class Switching to IgG and IgE by Recruitment of the HoxC4 and Oct-1 Homeodomain Proteins and Ku70/Ku86 to Newly Identified ATTT cis-Elements
J. Biol. Chem., June 13, 2003; 278(25): 23141 - 23150.
[Abstract] [Full Text] [PDF]

D. M. Willis, A. P. Loewy, N. Charlton-Kachigian, J.-S. Shao, D. M. Ornitz, and D. A. Towler
Regulation of Osteocalcin Gene Expression by a Novel Ku Antigen Transcription Factor Complex
J. Biol. Chem., September 27, 2002; 277(40): 37280 - 37291.
[Abstract] [Full Text] [PDF]

E. S. Sobel, M. Satoh, Y. Chen, E. K. Wakeland, and L. Morel
The Major Murine Systemic Lupus Erythematosus Susceptibility Locus Sle1 Results in Abnormal Functions of Both B and T Cells
J. Immunol., September 1, 2002; 169(5): 2694 - 2700.
[Abstract] [Full Text] [PDF]

W. Rodgers, S. J. Jordan, and J. D. Capra
Transient Association of Ku with Nuclear Substrates Characterized Using Fluorescence Photobleaching
J. Immunol., March 1, 2002; 168(5): 2348 - 2355.
[Abstract] [Full Text] [PDF]

J Bertinato, C Schild-Poulter, and R. Hache
Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits
J. Cell Sci., January 1, 2001; 114(1): 89 - 99.
[Abstract] [PDF]

Y.-T. Tai, G. Teoh, B. Lin, F. E. Davies, D. Chauhan, S. P. Treon, N. Raje, T. Hideshima, Y. Shima, K. Podar, et al.
Ku86 Variant Expression and Function in Multiple Myeloma Cells Is Associated with Increased Sensitivity to DNA Damage
J. Immunol., December 1, 2000; 165(11): 6347 - 6355.
[Abstract] [Full Text] [PDF]

K. N. Mahajan, L. Gangi-Peterson, D. H. Sorscher, J. Wang, K. N. Gathy, N. P. Mahajan, W. H. Reeves, and B. S. Mitchell
Association of terminal deoxynucleotidyl transferase with Ku
PNAS, November 23, 1999; 96(24): 13926 - 13931.
[Abstract] [Full Text] [PDF]

S. Galande and T. Kohwi-Shigematsu
Poly(ADP-ribose) Polymerase and Ku Autoantigen Form a Complex and Synergistically Bind to Matrix Attachment Sequences
J. Biol. Chem., July 16, 1999; 274(29): 20521 - 20528.
[Abstract] [Full Text] [PDF]

D. A. Shackelford, T. Tobaru, S. Zhang, and J. A. Zivin
Changes in Expression of the DNA Repair Protein Complex DNA-Dependent Protein Kinase after Ischemia and Reperfusion
J. Neurosci., June 15, 1999; 19(12): 4727 - 4738.
[Abstract] [Full Text] [PDF]

J. Wang, X. Dong, and W. H. Reeves
A Model for Ku Heterodimer Assembly and Interaction with DNA. IMPLICATIONS FOR THE FUNCTION OF Ku ANTIGEN
J. Biol. Chem., November 20, 1998; 273(47): 31068 - 31074.
[Abstract] [Full Text] [PDF]

N. A. Barlev, V. Poltoratsky, T. Owen-Hughes, C. Ying, L. Liu, J. L. Workman, and S. L. Berger
Repression of GCN5 Histone Acetyltransferase Activity via Bromodomain-Mediated Binding and Phosphorylation by the Ku-DNA-Dependent Protein Kinase Complex
Mol. Cell. Biol., March 1, 1998; 18(3): 1349 - 1358.
[Abstract] [Full Text]

J. Wang, X. Dong, K. Myung, E. A. Hendrickson, and W. H. Reeves
Identification of Two Domains of the p70 Ku Protein Mediating Dimerization with p80 and DNA Binding
J. Biol. Chem., January 9, 1998; 273(2): 842 - 848.
[Abstract] [Full Text] [PDF]

O. Osipovich, S. K. Durum, and K. Muegge
Defining the Minimal Domain of Ku80 for Interaction with Ku70
J. Biol. Chem., October 24, 1997; 272(43): 27259 - 27265.
[Abstract] [Full Text] [PDF]

P. Cortes, F. Weis-Garcia, Z. Misulovin, A. Nussenzweig, J.-S. Lai, G. Li, M.�C. Nussenzweig, and D. Baltimore
In vitro V(D)J recombination: Signal�joint�formation
PNAS, November 26, 1996; 93(24): 14008 - 14013.
[Abstract] [Full Text] [PDF]

Z. Han, N. Malik, T. Carter, W. H. Reeves, J. H. Wyche, and E. A. Hendrickson
DNA-dependent Protein Kinase Is a Target for a CPP32-like Apoptotic Protease
J. Biol. Chem., October 4, 1996; 271(40): 25035 - 25040.
[Abstract] [Full Text] [PDF]

Z. Han, C. Johnston, W. H. Reeves, T. Carter, J. H. Wyche, and E. A. Hendrickson
Characterization of a Ku86 Variant Protein That Results in Altered DNA Binding and Diminished DNA-dependent Protein Kinase Activity
J. Biol. Chem., June 14, 1996; 271(24): 14098 - 14104.
[Abstract] [Full Text] [PDF]

J. Fewell and E. Kuff
Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium
J. Cell Sci., January 7, 1996; 109(7): 1937 - 1946.
[Abstract] [PDF]

This Article

Summary

Full Text (PDF)

Alert me when this article is cited

Alert me if a correction is posted

Services

Email this article to a friend

Similar articles in this journal

Similar articles in PubMed

Alert me to new issues of the journal

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via Google Scholar

Google Scholar

Articles by Wang, J.

Articles by Reeves, W. H.

Search for Related Content

PubMed

PubMed Citation

Articles by Wang, J.

Articles by Reeves, W. H.

Social Bookmarking

What's this?

				F. Hammer, N. A. Compagnone, J.-L. Vigne, S. R. Bair, and S. H. Mellon Transcriptional Regulation of P450scc Gene Expression in the Embryonic Rodent Nervous System Endocrinology, February 1, 2004; 145(2): 901 - 912. [Abstract] [Full Text] [PDF]

				A. Schaffer, E. C. Kim, X. Wu, H. Zan, L. Testoni, S. Salamon, A. Cerutti, and P. Casali Selective Inhibition of Class Switching to IgG and IgE by Recruitment of the HoxC4 and Oct-1 Homeodomain Proteins and Ku70/Ku86 to Newly Identified ATTT cis-Elements J. Biol. Chem., June 13, 2003; 278(25): 23141 - 23150. [Abstract] [Full Text] [PDF]

				D. M. Willis, A. P. Loewy, N. Charlton-Kachigian, J.-S. Shao, D. M. Ornitz, and D. A. Towler Regulation of Osteocalcin Gene Expression by a Novel Ku Antigen Transcription Factor Complex J. Biol. Chem., September 27, 2002; 277(40): 37280 - 37291. [Abstract] [Full Text] [PDF]

				E. S. Sobel, M. Satoh, Y. Chen, E. K. Wakeland, and L. Morel The Major Murine Systemic Lupus Erythematosus Susceptibility Locus Sle1 Results in Abnormal Functions of Both B and T Cells J. Immunol., September 1, 2002; 169(5): 2694 - 2700. [Abstract] [Full Text] [PDF]

				W. Rodgers, S. J. Jordan, and J. D. Capra Transient Association of Ku with Nuclear Substrates Characterized Using Fluorescence Photobleaching J. Immunol., March 1, 2002; 168(5): 2348 - 2355. [Abstract] [Full Text] [PDF]

				J Bertinato, C Schild-Poulter, and R. Hache Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits J. Cell Sci., January 1, 2001; 114(1): 89 - 99. [Abstract] [PDF]

				Y.-T. Tai, G. Teoh, B. Lin, F. E. Davies, D. Chauhan, S. P. Treon, N. Raje, T. Hideshima, Y. Shima, K. Podar, et al. Ku86 Variant Expression and Function in Multiple Myeloma Cells Is Associated with Increased Sensitivity to DNA Damage J. Immunol., December 1, 2000; 165(11): 6347 - 6355. [Abstract] [Full Text] [PDF]

				K. N. Mahajan, L. Gangi-Peterson, D. H. Sorscher, J. Wang, K. N. Gathy, N. P. Mahajan, W. H. Reeves, and B. S. Mitchell Association of terminal deoxynucleotidyl transferase with Ku PNAS, November 23, 1999; 96(24): 13926 - 13931. [Abstract] [Full Text] [PDF]

				S. Galande and T. Kohwi-Shigematsu Poly(ADP-ribose) Polymerase and Ku Autoantigen Form a Complex and Synergistically Bind to Matrix Attachment Sequences J. Biol. Chem., July 16, 1999; 274(29): 20521 - 20528. [Abstract] [Full Text] [PDF]

				D. A. Shackelford, T. Tobaru, S. Zhang, and J. A. Zivin Changes in Expression of the DNA Repair Protein Complex DNA-Dependent Protein Kinase after Ischemia and Reperfusion J. Neurosci., June 15, 1999; 19(12): 4727 - 4738. [Abstract] [Full Text] [PDF]

				J. Wang, X. Dong, and W. H. Reeves A Model for Ku Heterodimer Assembly and Interaction with DNA. IMPLICATIONS FOR THE FUNCTION OF Ku ANTIGEN J. Biol. Chem., November 20, 1998; 273(47): 31068 - 31074. [Abstract] [Full Text] [PDF]

				N. A. Barlev, V. Poltoratsky, T. Owen-Hughes, C. Ying, L. Liu, J. L. Workman, and S. L. Berger Repression of GCN5 Histone Acetyltransferase Activity via Bromodomain-Mediated Binding and Phosphorylation by the Ku-DNA-Dependent Protein Kinase Complex Mol. Cell. Biol., March 1, 1998; 18(3): 1349 - 1358. [Abstract] [Full Text]

				J. Wang, X. Dong, K. Myung, E. A. Hendrickson, and W. H. Reeves Identification of Two Domains of the p70 Ku Protein Mediating Dimerization with p80 and DNA Binding J. Biol. Chem., January 9, 1998; 273(2): 842 - 848. [Abstract] [Full Text] [PDF]

				O. Osipovich, S. K. Durum, and K. Muegge Defining the Minimal Domain of Ku80 for Interaction with Ku70 J. Biol. Chem., October 24, 1997; 272(43): 27259 - 27265. [Abstract] [Full Text] [PDF]

				P. Cortes, F. Weis-Garcia, Z. Misulovin, A. Nussenzweig, J.-S. Lai, G. Li, M.�C. Nussenzweig, and D. Baltimore In vitro V(D)J recombination: Signal�joint�formation PNAS, November 26, 1996; 93(24): 14008 - 14013. [Abstract] [Full Text] [PDF]

				Z. Han, N. Malik, T. Carter, W. H. Reeves, J. H. Wyche, and E. A. Hendrickson DNA-dependent Protein Kinase Is a Target for a CPP32-like Apoptotic Protease J. Biol. Chem., October 4, 1996; 271(40): 25035 - 25040. [Abstract] [Full Text] [PDF]

				Z. Han, C. Johnston, W. H. Reeves, T. Carter, J. H. Wyche, and E. A. Hendrickson Characterization of a Ku86 Variant Protein That Results in Altered DNA Binding and Diminished DNA-dependent Protein Kinase Activity J. Biol. Chem., June 14, 1996; 271(24): 14098 - 14104. [Abstract] [Full Text] [PDF]

				J. Fewell and E. Kuff Intracellular redistribution of Ku immunoreactivity in response to cell-cell contact and growth modulating components in the medium J. Cell Sci., January 7, 1996; 109(7): 1937 - 1946. [Abstract] [PDF]