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Fig. 10 (3912). were coated overnight at 4degC with 50l fibronectin (10 g/ml). After blocking residual binding sites with BSA (0.1%, w/v, PBSA) 25l of DMEM containing nil antibody, 17 E6 ( v-blocking; 2g/ml), P1B5 ( 3-blocking; 1/50), P1D6 (5-blocking 1/50), P4C10 ( 1-blocking; 1/50) A11B2 (1-blocking; undiluted supernatant), 14E2 (class matched IgG1 control; 2 g/ml) or combinations of antibodies at similar concentrations were added. 51Cr-labelled V+B2 cells (1.5104in 25 l DMEM) were added to wells and the plate was incubated at 37degC for 75 minutes. After washing away unbound cells, the radioactivity (cpm) associated with the wells was determined. Percentage adhesion was calculated from quadruplicate samples. Data shown are one of two experiments which gave identical results. Bars represent \2611 s.d.in cell adhesion, migration matrix assembly and cytoskeletal organisation. J. Cell Biol 109, 863-875.[Abstract/Free Full Text]

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