Sperm exocytosis reconstructed in a cell-free system: evidence for the involvement of phospholipase C and actin filaments in membrane fusion

QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

This Article

	Summary
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager


Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Spungin, B.
	Articles by Breitbart, H.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Spungin, B.
	Articles by Breitbart, H.


Social Bookmarking

	What's this?

Beckers, C. J. M. and Balch, W. E (1989). Calcium and GTP: Essential components in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus. J. Cell Biol 108, 1245-1256.[Abstract/Free Full Text]

Beckers, C. J. M., Block, M. R., Glick, B. J., Rothman, J. E. and Balch, W. E (1989). Vesicular transport between the endoplasmic reticulum and the Golgi stack requires the NEM-sensitive fusion protein. Nature 339, 397-398.[Medline]

Ben-Av, P., Rubinstein, S. and Breitbart, H (1988). Induction of acrosomal reaction and calcium uptake in ram spermatozoa. Biochim. Biophys. Acta 939, 214-222.[Medline]

Bentley, J. K., Garbers, D. L., Domino, S. E., Noland, T. D. and Van Dop, C (1986). Spermatozoa contain a guanine nucleotide-binding protein ADP-ribosylated by Pertussis toxin. Biochem. Biophys. Res. Commun 138, 728-734.[Medline]

Berruti, G. and Franchi, M (1986). Calcium and polyphosphoinositides: Their distribution in relation to the membrane changes occurring in the head of boar spermatozoa. Eur. J. Cell Biol 41, 238-245.[Medline]

Bittner, M. A. and Holtz, R. W (1992). Kinetic analysis of secretion from permeabilized adrenal chromaffin cells reveals distinct components. J. Biol. Chem 267, 16219-16225.[Abstract/Free Full Text]

Bittner, M. A. and Holtz, R. W (1992). A temperature sensitive step in exocytosis. J. Biol. Chem 267, 16226-16229.[Abstract/Free Full Text]

Breitbart, H. and Rubinstein, S (1983). Calcium transport by bull spermatozoa plasma membranes. Biochim. Biophys. Acta 732, 464-468.[Medline]

Breitbart, H., Lax, J., Rotem, R. and Naor, Z (1992). Role of protein kinase C in the acrosome reaction of mammalian spermatozoa. Biochem. J 281, 473-476.

Burgoyne, R. D (1990). Intracellular membrane fusion in cell-free systems. Trends Biochem. Sci 15, 123-124.[Medline]

Burgoyne, R. D., Handel, S. E., Morgan, A., Rennison, M. E., Turner, M. D. and Wilde, C. J (1991). Calcium, the cytoskeleton and calpactin (annexin II) in exocytotic secretion from adrenal chromaffin and mammary epithelial cells. Biochem. Soc. Trans 19, 1085-1090.[Medline]

Carney D. H., Scott, D. L., Gordon, E. A and Labelle, E. F (1985). Phosphoinositides in mitogenesis: Neomycin inhibits thrombin-stimulated phosphoinositide turnover and initiation of cell proliferation. Cell 42, 479-488.[Medline]

Castellani-Ceresa, L., Brivio, M. F. and Radoelli, G (1992). F-Actin in acrosome reacted boar spermatozoa. Mol. Reprod. Dev 33, 99-107.[Medline]

Cheek, T. R. and Burgoyne, R. D (1986). Nicotine-evoked disassembly of cortical actin filaments in adrenal chromaffin cells. FEBS Lett 207, 110-114.[Medline]

Cockcroft, S. and Gomperts, B. D (1985). Role of guanine nucleotide binding protein in the activation of polyphophoinositide phosphodiesterase. Nature 314, 534-536.[Medline]

Cooper, C. L. and Holz, R. W (1993). Neomycin and spermine have similar effects on secretion and phosphoinositide metabolism in ATP depleted permeabilized adrenal chromaffin cells. Biochem. Biophys. Res. Commun 194, 1135-1142.[Medline]

Cooper, J. A (1987). Effects of cytochalasin and phalloidin on actin. J. Cell Biol 105, 1473-1478.[Free Full Text]

Cooper, J. A (1991). The role of actin polymerization in cell motility. Annu. Rev. Physiol 53, 585-605.[Medline]

Damjanov, I., Solter, D. and Knowles, B. B (1993). Functional epidermal growth factor receptor localizes to the post-acrosomal region of human spermatozoa. Biochem. Biophys. Res. Commun 190, 901-906.[Medline]

Diaz, R., Mayorga, L. S., Weidmann, P. J., Rothman, J. E. and Stahl, P. D (1989). Vesicle fusion following receptor-mediated endocytosis requires a protein active in Golgi transport. Nature 339, 398-400.[Medline]

Endo, Y., Lee, M. A. and Kopf, G. S (1987). Evidence for the role of a guanine nucleotide-binding regulatory protein in the zona pellucida induced mouse sperm acrosome reaction. Dev. Biol 119, 210-216.[Medline]

Endo, Y., Lee, M. A. and Kopf, G. S (1988). Characterization of an islet activating protein sensitive site in mouse sperm that is involved in the zona pellucida induced acrosome reaction. Dev. Biol 129, 12-24.[Medline]

Fleming, A. D. and Yanagamachi, R (1981). Effects of various lipids on the acrosome reaction and fertilizing capacity of guinea pig spermatozoa, with specific reference to the possible involvement of lysophospholipids in the acrosome reaction. Gamete Res 4, 253-273.

Florman, H. M., Tombes, R. M., First, N. L. and Babcock, D. F (1989). An adhesion-associated agonist from the zona pellucida activates G protein-promoted elevations of internal Ca2+and pH that mediate mammalian sperm acrosomal exocytosis. Dev. Biol 35, 133-146.

Glick, B. S. and Rothman, J. E (1987). Possible role for fatty acyl-coenzyme A in intracellular protein transport. Nature 326, 309-312.[Medline]

Goldschmidt-Clermont, P. J., Kim, J. W., Machesky, L. M., Rhee, S. G. and Pollard, T. D (1991). Regulation of phospholipase C-gamma 1 by profilin and tyrosine phosphorylation. Science 251, 1231-1233.[Abstract/Free Full Text]

Hildebrandt, J. D., Codina, J., Tash, J. S., Kirchick, H. J., Lipschutz, L. Sekura, R. D. and Birnbaumer, L (1985). The membrane bound spermtozoal adenyl cyclase system does not share coupling characteristics with somatic cell adenylyl cyclases. Endocrinology 116, 1356-1366.

Janmey, P. A. and Matsudaira, P. T (1988). Functional comparisons of Villin and Gelsolin. J. Biol. Chem 263, 16738-16743.[Abstract/Free Full Text]

Janmey, P. A (1994). Phosphoinositides and calcium as regulators of cellular actin assembly and disassembly. Annu. Rev. Physiol 56, 169-191.[Medline]

Koffer, A., Tatham, P. E. R. and Gomperts, B. D (1990). Changes in the state of actin during the exocytotic reaction of permeabilized rat mast cells. J. Cell Biol 111, 919-927.[Abstract/Free Full Text]

Kopf, G. S., Woolkalis, M. J. and Gerton, G. I (1986). Evidence for a guanine nucleotide binding protein in invertebrate and mammalian sperm. J. Biol. Chem 261, 7327-7331.[Abstract/Free Full Text]

Kopf, G. S. and Woolkalis, M. J (1991). ADP-ribosylation of G proteins with Pertussis toxin. Meth. Enzymol 195, 257-266.[Medline]

Kurioka, S. and Matsuda, N (1976). Phospholipase C assay using p-nitrophenylphosphorycholine together with sorbitol and its application to studying the metal and detergent requirements of the enzyme. Anal. Biochem 75, 281-289.[Medline]

Lax, Y., Grossman, S., Rubinstein, S., Magid, N. and Breitbart, H (1990). Role of lipoxygenase in the mechanism of acrosome reaction in mammalian sperm. Biochim. Biophys. Acta 1043, 12-18.[Medline]

Lax, Y., Rubinstein, S. and Breitbart, H (1994). Epidermal growth factor induces acrosomal exocytosis in bovine sperm. FEBS Lett 339, 234-238.[Medline]

Lelkes, P. I., Friedman, J. E., Rosenheck, K. and Oplatka, A (1986). Destabilization of actin filaments as a requirement for the secretion of catecholamines from permeabilized chromaffin cells. FEBS Lett 208, 357-363.[Medline]

Listedt, A. D. and Kelly, R. B (1987). Overcoming barriers to exocytosis. Trends Neurosci 10, 446-448.

Lowry, O. H., Rosebrough, N. H., Farr, A. L. and Randall, R. J (1951). Protein measurement with the folin reagent. Biol. Chem 193, 265-275.

Luk, A. S., Kaler, E. W. and Lee, S. P (1993). Phospholipase C induced aggregation and fusion of cholesterol-lecithin small unilamellar vesicles. Biochemistry 32, 6965-6973.[Medline]

Meisenhelder, J., Suh, P.-G., Rhee, S. G. and Hunter, T (1989). Phospholipase C-is a substrate for the PDGF and EGF receptor protein-tyrosine kinase in vivo and in vitro. Cell 57, 1109-1122.[Medline]

Miyamoto, S., Funatsu, T., Ischiwata, S. and Fujime, S (1993). Changes in mobility of chromaffin granules in actin network with its assembly and Ca2+-dependent disassembly by gelsolin. Biophys. J 64, 1139-1149.[Medline]

Nadine, C. Y., Rogers, J., Tomlinson, S. and Edwardson, J. M (1989). A specific interaction in vitro between pancreatic zymogen granules and plasma membranes: Stimulation by G-protein but not Ca2+. J. Cell Biol 109, 2801-2808.[Abstract/Free Full Text]

Naz, R. K. and Ahmed, K (1992). Presence of expression products of c-erbB-1 and c-erbB-2/HER2 genes on mammalian sperm cell, and effects of their regulation on fertilization. J. Reprod. Immunol 21, 223-239.[Medline]

Parks, J. E., Arion, J. W. and Foote, R. H (1987). Lipids of plasma membrane and outer acrosomal membrane from bovine spermatozoa. Biol. Reprod 37, 1249-1258.[Abstract]

Parrish, J. J., Susko-Parrish, J. L., Winer, M. A. and First, N (1988). Capacitation of bovine sperm by heparin. Biol. Reprod 38, 1171-1180.[Abstract]

Passador, L. and Iglewski, W (1994). ADP-ribosylating toxins. Meth. Enzymol 235, 617-631.[Medline]

Peterson, R. N., Bozzola, J. J., Hunt, W. P. and Darabi, A (1990). Characterization of membrane-associated actin in boar spermatozoa. J. Exp. Zool 253, 202-214.[Medline]

Rana, R. S. and Hokin, L. E (1990). Role of phosphoinositides in transmembrane signalling. Physiol. Rev 70, 115-164.[Free Full Text]

Rhee, S. G (1991). Inositol phospholipid-specific phospholipase C: Interaction of the1 isoform with tyrosine kinase. Trends Biochem. Sci 16, 297-301.[Medline]

Rijken, P. J., Hage, W. J., van Bergen en Henegouwen, P. M. P., Verkleij, A. J. and Boonstra, J (1991). Epidermal growth factor induces rapid reorganization of the actin microfilament system in human A431 cells. J. Cell Sci 100, 491-499.[Abstract/Free Full Text]

Roldan, E. R. S. and Harrison, R. A. P (1989). Polyphosphoinositide breakdown and subsequent exocytosis in the Ca2+/ionophore-induced acrosome reaction in mammalian spermatozoa. Biochem. J 259, 397-406.[Medline]

Rotem, R., Paz, G. F., Homonnai, Z. T., Kalina, M. and Naor, Z (1990). Protein kinase C is present on human sperm: possible role in flagellar motility. Proc. Nat. Acad. Sci. USA 87, 7305-7308.[Abstract/Free Full Text]

Rotem, R., Paz, G. F., Homonnai, Z. T., Kalina, M., Lax, J., Breitbart, H. and Naor, Z (1992). Ca2+independent induction of acrosome reaction by protein kinase C in human sperm. Endocrinology 131, 2235-2243.[Abstract/Free Full Text]

Rubinstein, S. and Breitbart, H (1991). Role of spermine in mammalian sperm capacitation and acrosome reaction. Biochem. J 278, 25-28.

Scarpa, A (1979). Measurements of cation transport with metalochromic indicators. Meth. Enzymol 56, 301-388.[Medline]

Siegel, D. P., Banschbach, J., Alford, D., Ellens, H., Lis, L. J., Quinn, P. J., Yeagle, P. L. and Bentz, J (1989). Physiological levels of diacylglycerols in phospholipid membranes induce membrane fusion and stabilize inverted phases. Biochemistry 28, 3703-3709.[Medline]

Spiegel, A. M., Shenker, A. and Weinstein, L. S (1992). Receptor-effector coupling by G-proteins: Implications for normal and abnormal signal transduction. Endocrine Rev 13, 1-30.

Spungin, B., Levinshal, T., Rubinstein, S. and Breitbart, H (1992). A cell free system reveals that capacitation is a prerequisite for membrane fusion during the acrosome reaction. FEBS Lett 311, 155-160.[Medline]

Spungin, B., Margalit, I. and Breitbart, H (1995). A 70 kD protein istransferred from the outer acrosomal to the plasma membrane during capcitation. FEBS Lett 357, 98-102.[Medline]

Thomas, P. and Meizel, S (1989). Phosphatidylinositol-4, 5-bisphosphate hydrolysis in human sperm stimulated with follicular fluid or progesterone is dependent upon Ca2+influx. Biochem. J 264, 539-546.[Medline]

van Bergen en Henegouwen, P. M. P., Den Hartigh, J. C., Romeyn, P., Verkleij, A. J. and Boonstra, J (1992). The epidermal growth factor receptor is associated with actin filaments. Exp. Cell. Res 199, 90-97.[Medline]

Vijayasarathy, S., Shivaji, S. and Balaram, P (1982). Bull sperm plasma and acrosomal membranes: Fluorescence studies of lipid phase activity. Biochem. Biophys. Res. Commun 108, 585-591.[Medline]

Vitale, M. L., Rodriguez-Del Castillo, A., Tchakarov, L. and Trifaro, J. M (1991). Cortical filamentous actin disassembly and scinderin redistribution during chromaffin cell stimulation precede exocytosis, a phenomenon not exhibited by gelsolin. J. Cell Biol 113, 1057-1067.[Abstract/Free Full Text]

Watson, P. F. and Plummer, J. M (1986). Relationship between calcium binding sites and membrane fusion during the acrosome reaction induced by ionophore in ram spermatozoa. J. Exp. Zool 238, 113-118.[Medline]

Weidman, P. J., Melancon, P., Block, M. M. and Rothman, J. E (1989). Binding of an N-ethylmaleimide sensitive fusion protein to Golgi membranes requires both a soluble protein(s) and an integral membrane receptor. J. Cell Biol 108, 1589-1596.[Abstract/Free Full Text]

Woodman, P. G. and Warren, G (1988). Fusion between vesicles from the pathway of receptor-mediated endocytosis in a cell free system. Eur. J. Biochem 173, 101-108.[Medline]

Yang, L. J., Rhee, S. G. and Williamson, J. R (1994). Epidermal growth factor-induced activation and translocation of phospholipase C-1 to the cytoskeleton in rat hepatocytes. J. Biol. Chem 269, 7156-7162.[Abstract/Free Full Text]

Zahler, W. L. and Doak, G. A (1975). Isolation of the outer acrosomal membrane from bull sperm. Biochim. Biophys. Acta 406, 479-488.[Medline]

Zhu, P. C., Thureson-Klein, A. and Klein, R. L (1986). Exocytosis from large-dense cored vesicles outside the active synaptic zones of terminals within the trigeminal subnucleus caudalis: a possible mechanism for neuropeptide release. Neuroscience 19, 43-54.[Medline]

CiteULike

Complore

Connotea

Del.icio.us Add to Digg

Digg

Technorati

Twitter What's this?

This article has been cited by other articles:

T. Almog, S. Lazar, N. Reiss, N. Etkovitz, E. Milch, N. Rahamim, M. Dobkin-Bekman, R. Rotem, M. Kalina, J. Ramon, et al.
Identification of Extracellular Signal-regulated Kinase 1/2 and p38 MAPK as Regulators of Human Sperm Motility and Acrosome Reaction and as Predictors of Poor Spermatozoan Quality
J. Biol. Chem., May 23, 2008; 283(21): 14479 - 14489.
[Abstract] [Full Text] [PDF]

N. Etkovitz, S. Rubinstein, L. Daniel, and H. Breitbart
Role of PI3-Kinase and PI4-Kinase in Actin Polymerization During Bovine Sperm Capacitation
Biol Reprod, August 1, 2007; 77(2): 263 - 273.
[Abstract] [Full Text] [PDF]

H. Harayama, T. Murase, and M. Miyake
A Cyclic Adenosine 3',5'-Monophosphate Stimulates Phospholipase C{gamma}1-Calcium Signaling via the Activation of Tyrosine Kinase in Boar Spermatozoa
J Androl, November 1, 2005; 26(6): 732 - 740.
[Abstract] [Full Text] [PDF]

D. M. Hutt, J. M. Baltz, and J. K. Ngsee
Synaptotagmin VI and VIII and Syntaxin 2 Are Essential for the Mouse Sperm Acrosome Reaction
J. Biol. Chem., May 27, 2005; 280(21): 20197 - 20203.
[Abstract] [Full Text] [PDF]

D.Y. Liu, G.N. Clarke, and H.W.G. Baker
Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding
Hum. Reprod., April 1, 2005; 20(4): 999 - 1005.
[Abstract] [Full Text] [PDF]

H. Breitbart, G. Cohen, and S. Rubinstein
Role of actin cytoskeleton in mammalian sperm capacitation and the acrosome reaction
Reproduction, March 1, 2005; 129(3): 263 - 268.
[Abstract] [Full Text] [PDF]

S. H. Benoff, C. Millan, I. R. Hurley, B. Napolitano, and J. L. Marmar
Bilateral increased apoptosis and bilateral accumulation of cadmium in infertile men with left varicocele
Hum. Reprod., March 1, 2004; 19(3): 616 - 627.
[Abstract] [Full Text] [PDF]

R. D. Burgoyne and A. Morgan
Secretory Granule Exocytosis
Physiol Rev, April 1, 2003; 83(2): 581 - 632.
[Abstract] [Full Text] [PDF]

E. Brener, S. Rubinstein, G. Cohen, K. Shternall, J. Rivlin, and H. Breitbart
Remodeling of the Actin Cytoskeleton During Mammalian Sperm Capacitation and Acrosome Reaction
Biol Reprod, March 1, 2003; 68(3): 837 - 845.
[Abstract] [Full Text] [PDF]

D.Y. Liu, M. Martic, G.N. Clarke, I. Grkovic, C. Garrett, M.E. Dunlop, and H.W.G. Baker
An anti-actin monoclonal antibody inhibits the zona pellucida-induced acrosome reaction and hyperactivated motility of human sperm
Mol. Hum. Reprod., January 1, 2002; 8(1): 37 - 47.
[Abstract] [Full Text] [PDF]

E. Dragileva, S. Rubinstein, and H. Breitbart
Intracellular Ca2+-Mg2+-ATPase Regulates Calcium Influx and Acrosomal Exocytosis in Bull and Ram Spermatozoa
Biol Reprod, November 1, 1999; 61(5): 1226 - 1234.
[Abstract] [Full Text]

D.Y. Liu, M. Martic, G.N. Clarke, M.E. Dunlop, and H.W.G. Baker
An important role of actin polymerization in the human zona pellucida-induced acrosome reaction
Mol. Hum. Reprod., October 1, 1999; 5(10): 941 - 949.
[Abstract] [Full Text] [PDF]

R.-M. Pelletier, J.-M. Trifaro, M. E. Carbajal, Y. Okawara, and M. L. Vitale
Calcium-Dependent Actin Filament-Severing Protein Scinderin Levels and Localization in Bovine Testis, Epididymis, and Spermatozoa
Biol Reprod, May 1, 1999; 60(5): 1128 - 1136.
[Abstract] [Full Text]

K. Becker and N. Hart
Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis
J. Cell Sci., January 1, 1999; 112(1): 97 - 110.
[Abstract] [PDF]

A. Revelli, M. Massobrio, and J. Tesarik
Nongenomic Actions of Steroid Hormones in Reproductive Tissues
Endocr. Rev., February 1, 1998; 19(1): 3 - 17.
[Abstract] [Full Text]

B Spungin and H Breitbart
Calcium mobilization and influx during sperm exocytosis
J. Cell Sci., January 7, 1996; 109(7): 1947 - 1955.
[Abstract] [PDF]

This Article

Summary

Full Text (PDF)

Alert me when this article is cited

Alert me if a correction is posted

Services

Email this article to a friend

Similar articles in this journal

Similar articles in PubMed

Alert me to new issues of the journal

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via Google Scholar

Google Scholar

Articles by Spungin, B.

Articles by Breitbart, H.

Search for Related Content

PubMed

PubMed Citation

Articles by Spungin, B.

Articles by Breitbart, H.

Social Bookmarking

What's this?

				N. Etkovitz, S. Rubinstein, L. Daniel, and H. Breitbart Role of PI3-Kinase and PI4-Kinase in Actin Polymerization During Bovine Sperm Capacitation Biol Reprod, August 1, 2007; 77(2): 263 - 273. [Abstract] [Full Text] [PDF]

				H. Harayama, T. Murase, and M. Miyake A Cyclic Adenosine 3',5'-Monophosphate Stimulates Phospholipase C{gamma}1-Calcium Signaling via the Activation of Tyrosine Kinase in Boar Spermatozoa J Androl, November 1, 2005; 26(6): 732 - 740. [Abstract] [Full Text] [PDF]

				D. M. Hutt, J. M. Baltz, and J. K. Ngsee Synaptotagmin VI and VIII and Syntaxin 2 Are Essential for the Mouse Sperm Acrosome Reaction J. Biol. Chem., May 27, 2005; 280(21): 20197 - 20203. [Abstract] [Full Text] [PDF]

				D.Y. Liu, G.N. Clarke, and H.W.G. Baker Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding Hum. Reprod., April 1, 2005; 20(4): 999 - 1005. [Abstract] [Full Text] [PDF]

				H. Breitbart, G. Cohen, and S. Rubinstein Role of actin cytoskeleton in mammalian sperm capacitation and the acrosome reaction Reproduction, March 1, 2005; 129(3): 263 - 268. [Abstract] [Full Text] [PDF]

				S. H. Benoff, C. Millan, I. R. Hurley, B. Napolitano, and J. L. Marmar Bilateral increased apoptosis and bilateral accumulation of cadmium in infertile men with left varicocele Hum. Reprod., March 1, 2004; 19(3): 616 - 627. [Abstract] [Full Text] [PDF]

				R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]

				E. Brener, S. Rubinstein, G. Cohen, K. Shternall, J. Rivlin, and H. Breitbart Remodeling of the Actin Cytoskeleton During Mammalian Sperm Capacitation and Acrosome Reaction Biol Reprod, March 1, 2003; 68(3): 837 - 845. [Abstract] [Full Text] [PDF]

				D.Y. Liu, M. Martic, G.N. Clarke, I. Grkovic, C. Garrett, M.E. Dunlop, and H.W.G. Baker An anti-actin monoclonal antibody inhibits the zona pellucida-induced acrosome reaction and hyperactivated motility of human sperm Mol. Hum. Reprod., January 1, 2002; 8(1): 37 - 47. [Abstract] [Full Text] [PDF]

				E. Dragileva, S. Rubinstein, and H. Breitbart Intracellular Ca2+-Mg2+-ATPase Regulates Calcium Influx and Acrosomal Exocytosis in Bull and Ram Spermatozoa Biol Reprod, November 1, 1999; 61(5): 1226 - 1234. [Abstract] [Full Text]

				D.Y. Liu, M. Martic, G.N. Clarke, M.E. Dunlop, and H.W.G. Baker An important role of actin polymerization in the human zona pellucida-induced acrosome reaction Mol. Hum. Reprod., October 1, 1999; 5(10): 941 - 949. [Abstract] [Full Text] [PDF]

				R.-M. Pelletier, J.-M. Trifaro, M. E. Carbajal, Y. Okawara, and M. L. Vitale Calcium-Dependent Actin Filament-Severing Protein Scinderin Levels and Localization in Bovine Testis, Epididymis, and Spermatozoa Biol Reprod, May 1, 1999; 60(5): 1128 - 1136. [Abstract] [Full Text]

				K. Becker and N. Hart Reorganization of filamentous actin and myosin-II in zebrafish eggs correlates temporally and spatially with cortical granule exocytosis J. Cell Sci., January 1, 1999; 112(1): 97 - 110. [Abstract] [PDF]

				A. Revelli, M. Massobrio, and J. Tesarik Nongenomic Actions of Steroid Hormones in Reproductive Tissues Endocr. Rev., February 1, 1998; 19(1): 3 - 17. [Abstract] [Full Text]

				B Spungin and H Breitbart Calcium mobilization and influx during sperm exocytosis J. Cell Sci., January 7, 1996; 109(7): 1947 - 1955. [Abstract] [PDF]