The role of the ran GTPase in nuclear assembly and DNA replication: characterisation of the effects of Ran mutants

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Avis, J. A. and Clarke, P. R (1996). Ran, a GTPase involved in nuclear processes: its regulators and effectors. J. Cell Sci 109, 2423-2427.[Abstract]

Bischoff, F. R. and Ponstingl, H (1991). Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 354, 80-82.[Medline]

Bischoff, F. R. and Ponstingl, H (1991). Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Nat. Acad. Sci. USA 88, 10830-10834.[Abstract/Free Full Text]

Bischoff, F. R., Klebe, C., Kretschmer, J., Wittinghofer, A. and Ponstingl, H (1994). RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Nat. Acad. Sci. USA 91, 2587-2591.[Abstract/Free Full Text]

Bischoff, F. R. and Ponstingl, H (1995). Catalysis of guanine nucleotide exchange of Ran by RCC1 and stimulation of hydrolysis of Ran-bound GTP by Ran-GAP1. Meth. Enzymol 257, 135-144.[Medline]

Bischoff, F. R., Krebber, H., Smirnova, E., Dong, W. and Ponstingl, H (1995). Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J 14, 705-715.[Medline]

Bischoff, F. R. and G\232rlich, D (1997). RanBP1 is crucial for the release of RanGTP from importin-related nuclear transport factors. FEBS Lett 419, 249-254.[Medline]

Blow, J. J. and Laskey, R. A (1986). Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. Cell 47, 577-587.[Medline]

Carey, K. L., Richards, S. A., Lounsbury, K. M. and Macara, I. G (1996). Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear-protein import. J. Cell Biol 133, 985-996.[Abstract/Free Full Text]

Clarke, P. R., Klebe, C., Wittinghofer, A. and Karsenti, E (1995). Regulation of Cdc2/cyclin B activation by Ran, a Ras-related GTPase. J. Cell Sci 108, 1217-1225.[Abstract]

Clarkson, W. D., Kent, H. M. and Stewart, M (1996). Separate binding-sites on nuclear transport factor-2 (NTF2) for GDP-Ran and the phenylalanine-rich repeat regions of nucleoporins p62 and Nsp1p. J. Mol. Biol 263, 517-524.[Medline]

Coutavas, E., Ren, M., Oppenheim, J. D., D'Eustachio, P. and Rush, M. G (1993). Characterization of proteins that interact with the cell-cycle regulatory protein Ran/TC4. Nature 366, 585-587.[Medline]

Cox, L. S (1992). DNA replication in cell-free extracts from Xenopus eggs is prevented by disrupting nuclear envelope function. J. Cell Sci 101, 43-53.[Abstract/Free Full Text]

Dasso, M., Seki, T., Azuma, Y., Ohba, T. and Nishimoto, T (1994). A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation. EMBO J 13, 5732-5744.[Medline]

Drivas, G. T., Shih, A., Coutavas, E., Rush, M. G. and D'Eustachio, P (1990). Characterization of four novel ras -like genes expressed in a human teratocarcinoma cell line. Mol. Cell. Biol 10, 1793-1798.[Abstract/Free Full Text]

Ellis, D. J., Jenkins, H., Whitfield, W. G. and Hutchison, C. J (1997). GST-lamin fusion proteins act as dominant negative mutants in Xenopus egg extract and reveal the function of the lamina in DNA replication. J. Cell Sci 110, 2507-2518.[Abstract]

Finlay, D. R. and Forbes, D. J (1990). Reconstitution of biochemically altered nuclear pores:transport can be eliminated and restored. Cell 60, 17-29.[Medline]

Floer, M. and Blobel, G (1996). The nuclear transport factor karyopherin beta binds stoichiometrically to Ran-GTP and inhibits the Ran GTPase activating protein. J. Biol. Chem 271, 5313-5316.[Abstract/Free Full Text]

Fornerod, M., Ohno, M., Yoshida, M. and Mattai, I. W (1997). Crm1 is an export factor for leucine-rich nuclear export signals. Cell 90, 1051-1060.[Medline]

Fukuda, M., Asano, S., Nakamura, T., Adachi, M., Yoshida, M., Yanagida, M. and Nishida, E (1997). CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature 390, 308-311.[Medline]

G\232rlich, D., Kostka, S., Kraft, R., Dingwall, C., Laskey, R., Hartmann, E. and Prehn, S (1995). Two different subunits of importin cooperate to recognise nuclear localisation signals and bind them to the nuclear envelope. Curr. Biol 5, 383-392.[Medline]

G\232rlich, D. and Mattaj, I. W (1996). Nucleocytoplasmic transport. Science 271, 1513-1518.[Abstract]

G\232rlich, D., Kraft, R., Kostka, S., Vogel, F., Hartmann, E., Laskey, R. A., Mattaj, I. W. and Izaurralde, E (1996). Importin provides a link between nuclear protein import and U snRNA export. Cell 87, 27-32.

G\232rlich, D., Pante, N., Kutay, U., Aebi, U. and Bischoff, F. R (1996). Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J 15, 5584-5594.[Medline]

Hutchison, C. J., Cox, R. C. and Ford, C. C (1988). The control of DNA replication in a cell free extract that recapitulates a basic cell cycle in vitro. Development 103, 553-566.[Abstract]

Izaurralde, E., Kutay, U., von Kobbe, C., Mattai, I. W. and G\232rlich, D (1997). The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus. EMBO J 16, 6535-6547.[Medline]

Klebe, C., Nishimoto, T. and Wittinghofer, A (1993). Functional expression in Escherichia coli of the mitotic regulator proteins p24ranand p45rcc1and fluorescence measurements of their interaction. Biochemistry 32, 11923-11928.[Medline]

Klebe, C., Bischoff, F. R., Ponstingl, H. and Wittinghofer, A (1995). Interaction of the nuclear GTP-binding protein Ran with its regulatory proteins RCC1 and RanGAP1. Biochemistry 34, 639-647.[Medline]

Klebe, C., Prinz, H., Wittinghofer, A. and Goody, R. S (1995). The kinetic mechanism of Ran--nucleotide exchange catalyzed by RCC1. Biochemistry 34, 12543-12552.[Medline]

Kornbluth, S., Dasso, M. and Newport, J (1994). Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. J. Cell Biol 125, 705-719.[Abstract/Free Full Text]

Kutay, U., Bischoff, F. R., Kostka, S., Kraft, R. and G\232rlich, D (1997). Export of importinfrom the nucleus is mediated by a specific nuclear transport factor. Cell 90, 1061-1071.[Medline]

Kutay, U., Lipowsky, G., Izaurralde, E., Bischoff, F. R., Scharzmaier, P., Hartmann, E. and G\232rlich, D (1998). Identification of a tRNA-specific nuclear export receptor. Molecular Cell 1, 359-369.

Lounsbury, K. M., Richards, S. A., Perlungher, R. R. and Macara, I. G (1996). Ran binding domains promote the interaction of Ran with p97/beta-karyopherin, linking the docking and translocation steps of nuclear import. J. Biol. Chem 271, 2357-2360.[Abstract/Free Full Text]

Lounsbury, K. M. and Macara, I. G (1997). Ran-binding protein 1 (RanBP1) forms a ternary complex with Ran and karyopherinand reduces Ran GTPase-activating protein (RanGAP) inhibition by karyopherin. J. Biol. Chem 272, 551-555.[Abstract/Free Full Text]

Mahajan, R., Delphin, C., Guan, T., Gerace, L. and Melchior, F (1997). A small ubiquitin-related polypeptide involved in targetting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107.[Medline]

Matunis, M. J., Coutavas, E. and Blobel, G (1996). A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell Biol 135, 1457-1470.[Abstract/Free Full Text]

Melchior, F., Guan, T., Yokoyama, N., Nishimoto, T. and Gerace, L (1995). GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol 131, 571-581.[Abstract/Free Full Text]

Moore, M. S. and Blobel, G (1994). Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Nat. Acad. Sci. USA 91, 10212-10216.[Abstract/Free Full Text]

Nehrbass, U. and Blobel, G (1996). Role of the nuclear transport factor p10 in nuclear import. Science 272, 120-122.[Abstract]

Neville, M., Stutz, F., Lee, L., Davis, L. I. and Rosbash, M (1997). The importin-beta family member Crm1p bridges the interaction between rev and the nucler pore complex during nuclear export. Curr. Biol 7, 767-775.[Medline]

Nicol\207s, F. J., Zhang, C., Hughes, M., Goldberg, M. W., Watton, S. J. and Clarke, P. R (1997). Xenopus Ran-binding protein 1: molecular interactions and effects on nuclear assembly in Xenopus egg extracts. J. Cell Sci 110, 3019-3030.[Abstract]

Ohtsubo, M., Kai, R., Furuno, N., Sekiguchi, T., Sekiguchi, M., Hayashida, H., Kuma, K., Miyata, T., Fukushige, S., Murotsu, T., Matsubara, K. and Nishimoto, T (1987). Isolation and characterisation of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation. Genes Dev 1, 585-593.[Abstract/Free Full Text]

Ossareh-Nazari, B., Bachelerie, F. and Dargemont, C (1997). Evidence for a role of Crm1 in signal-mediated nuclear protein export. Science 278, 141-144.[Abstract/Free Full Text]

Palacios, I., Weis, K., Klebe, C., Mattaj, I. W. and Dingwall, C (1996). Ran/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus. J. Cell Biol 133, 485-494.[Abstract/Free Full Text]

Paschal, B. M. and Gerace, L (1995). Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell Biol 129, 925-937.[Abstract/Free Full Text]

Paschal, B. M., Delphin, C. and Gerace, L (1996). Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97. Proc. Nat. Acad. Sci. USA 93, 7679-7683.[Abstract/Free Full Text]

Philpott, A., Leno, G. H. and Laskey, R. A (1991). Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin. Cell 65, 569-578.[Medline]

Pu, R. T. and Dasso, M (1997). The balance of RanBP1 and RCC1 is critical for nuclear assembly and nuclear transport. Mol. Biol. Cell 8, 1955-1970.[Abstract/Free Full Text]

Rexach, R. and Blobel, G (1995). Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83, 683-692.[Medline]

Richards, S. A., Lounsbury, K. M., Carey, K. L. and Macara, I. G (1996). A nuclear export signal is essential for the cytosolic localization of the Ran binding protein, RanBP1. J. Cell Biol 134, 1157-1168.[Abstract/Free Full Text]

Richards, S. A., Carey, K. L. and Macara, I. G (1997). Requirement of guanosine triphosphate-bound Ran for signal-mediated nuclear protein export. Science 276, 1842-1848.[Abstract/Free Full Text]

Rush, M. G., Drivas, G. and D'Eustachio, P. D (1996). The small nuclear GTPase Ran: how much does it run?. BioEssays 18, 103-112.[Medline]

Saitoh, H., Cooke, C. A., Burgess, W. H., Earnshaw, W. C. and Dasso, M (1996). Direct and indirect association of the small GTPase Ran with nuclear-pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts. Mol. Biol. Cell 7, 1319-1334.[Abstract]

Saitoh, H., Pu, R., Cavenagh, M. and Dasso, M (1997). RanBP2 associates with Ubc9p and a modified form of RanGAP1. Proc. Nat. Acad. Sci. USA 94, 3736-3741.[Abstract/Free Full Text]

Schlenstedt, G., Saavedra, C., Loeb, J. D., Cole, C. N. and Silver, P. A (1995). The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Proc. Nat. Acad. Sci. USA 92, 225-229.[Abstract/Free Full Text]

Stade, K., Ford, C. S., Guthrie, C. and Weis, K (1997). Exportin 1 (Crm1p) is an essential nuclear export factor. Cell 90, 1041-1050.[Medline]

Weis, K., Mattaj, I. W. and Lamond, A. I (1995). Identification of hSrp1-alpha as a functional receptor for nuclear-localization sequences. Science 268, 1049-1053.[Abstract/Free Full Text]

Weis, K., Dingwall, C. and Lamond, A. I (1996). Characterization of the nuclear protein import mechanism using Ran mutants with altered nucleotide binding specificities. EMBO J 15, 7120-7128.[Medline]

Wu, J., Matunis, M. J., Kraemer, D., Blobel, G. and Coutavas, E (1995). Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem 270, 14209-14213.[Abstract/Free Full Text]

Yokoyama, N., Hayashi, N., Seki, T., Pante, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U., Fukui, M. and Nishimoto, T (1995). A giant nucleopore protein that binds Ran/TC4. Nature 376, 184-188.[Medline]

Zhang, C., Jenkins, H., Goldberg, M. W., Allen, T. D. and Hutchison, C. J (1996). Nuclear lamina and nuclear matrix organisation in sperm pronuclei assembled in Xenopus egg extract. J. Cell Sci 109, 2275-2286.[Abstract]

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				C. K. Lau, V. A. Delmar, R. C. Chan, Q. Phung, C. Bernis, B. Fichtman, B. A. Rasala, and D. J. Forbes Transportin Regulates Major Mitotic Assembly Events: From Spindle to Nuclear Pore Assembly Mol. Biol. Cell, September 15, 2009; 20(18): 4043 - 4058. [Abstract] [Full Text] [PDF]

				C. F. Woeller, D. D. Anderson, D. M. E. Szebenyi, and P. J. Stover Evidence for Small Ubiquitin-like Modifier-dependent Nuclear Import of the Thymidylate Biosynthesis Pathway J. Biol. Chem., June 15, 2007; 282(24): 17623 - 17631. [Abstract] [Full Text] [PDF]

				R. V. Silverman-Gavrila and A. Wilde Ran Is Required before Metaphase for Spindle Assembly and Chromosome Alignment and after Metaphase for Chromosome Segregation and Spindle Midbody Organization Mol. Biol. Cell, April 1, 2006; 17(4): 2069 - 2080. [Abstract] [Full Text] [PDF]

				Y.-K. Cao, Z.-S. Zhong, D.-Y. Chen, G.-X. Zhang, H. Schatten, and Q.-Y. Sun Cell cycle-dependent localization and possible roles of the small GTPase Ran in mouse oocyte maturation, fertilization and early cleavage Reproduction, October 1, 2005; 130(4): 431 - 440. [Abstract] [Full Text] [PDF]

				T. A. Prokhorova, K. Mowrer, C. H. Gilbert, and J. C. Walter DNA replication of mitotic chromatin in Xenopus egg extracts PNAS, November 11, 2003; 100(23): 13241 - 13246. [Abstract] [Full Text] [PDF]

				A. Narayanan, J. Eifert, K. A. Marfatia, I. G. Macara, A. H. Corbett, R. M. Terns, and M. P. Terns Nuclear RanGTP is not required for targeting small nucleolar RNAs to the nucleolus J. Cell Sci., January 1, 2003; 116(1): 177 - 186. [Abstract] [Full Text] [PDF]

				G. Timinszky, L. Tirian, F. T. Nagy, G. Toth, A. Perczel, Z. Kiss-Laszlo, I. Boros, P. R. Clarke, and J. Szabad The importin-{beta} P446L dominant-negative mutant protein loses RanGTP binding ability and blocks the formation of intact nuclear envelope J. Cell Sci., April 15, 2002; 115(8): 1675 - 1687. [Abstract] [Full Text] [PDF]

				F. J. Nicolas, W. J. Moore, C. Zhang, and P. R. Clarke XMog1, a nuclear Ran-binding protein in Xenopus, is a functional homologue of Schizosaccharomyces pombe Mog1p that co-operates with RanBP1 to control generation of Ran-GTP J. Cell Sci., March 10, 2002; 114(16): 3013 - 3023. [Abstract] [Full Text] [PDF]

				C. Zhang and P. R. Clarke Chromatin-Independent Nuclear Envelope Assembly Induced by Ran GTPase in Xenopus Egg Extracts Science, May 26, 2000; 288(5470): 1429 - 1432. [Abstract] [Full Text]

				S Sazer and M Dasso The ran decathlon: multiple roles of Ran J. Cell Sci., January 4, 2000; 113(7): 1111 - 1118. [Abstract] [PDF]

				I. Novoa, M. G. Rush, and P. D'Eustachio Isolated Mammalian and Schizosaccharomyces pombe Ran-binding Domains Rescue S. pombe sbp1 (RanBP1) Genomic Mutants Mol. Biol. Cell, July 1, 1999; 10(7): 2175 - 2190. [Abstract] [Full Text]