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First published online 1 April 2003
doi: 10.1242/jcs.00423

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Spatial and temporal dynamics of budding yeast mitochondria lacking the division component Fis1p

Stefan Jakobs^1,*,, Nadia Martini^1,*, Astrid C. Schauss¹, Alexander Egner¹, Benedikt Westermann² and Stefan W. Hell¹

¹ Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany
² Institut für Physiologische Chemie, Universität München, Butenandtstrasse 5, 81377 München, Germany

Author for correspondence (e-mail: sjakobs{at}gwdg.de)

Accepted 11 February 2003

The mitochondrial compartment of budding yeast (Saccharomyces cerevisiae) is a highly dynamic net-like structure of tubules that constantly undergo fusion and fission. The outer membrane protein Fis1p plays a crucial role in mitochondrial fission. Here we report on the temporal and spatial dynamics of this organelle in wild-type cells and in fis1 mutants. Mitochondria of fis1 mutants adapt their mitochondrial network to a change in carbon source. We find that the frequencies of apparent matrix separation and fusion events decrease in both wild-type cells and in mutants lacking Fis1p upon glucose repression. Matrix separation could be caused by matrix constriction and does not necessarily require fission of the inner or outer membrane. Double-labelling experiments demonstrated that some of these matrix separations in fis1 mutants are due to genuine tubule fissions, whereas others do not involve fission of the outer membrane. The rates of matrix separation in fis1 mutants almost approach those of the wildtype, demonstrating that, although apparently involved in outer membrane fission, Fis1p is not crucial for the separation of the mitochondrial matrix. In mutants lacking the GTPase Dnm1p no complete tubule fissions were recorded, although dnm1 mutants display matrix separations as well. The data suggest that different molecular machineries are responsible for the separation of the matrix and the fission of the outer membrane in budding yeast.

Key words: Saccharomyces cerevisiae, Fluorescence microscopy, Glucose repression, Membrane fission, Organelle morphology

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