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First published online 23 April 2003
doi: 10.1242/jcs.00526

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Rapid transport of neural intermediate filament protein

Brian T. Helfand^*, Patty Loomis^*, Miri Yoon and Robert D. Goldman

Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, 303 East Chicago Avenue, Ward 11-145, Chicago, IL 60611, USA

Author for correspondence (e-mail: r-goldman{at}northwestern.edu)

Accepted 1 April 2003

Peripherin is a neural intermediate filament protein that is expressed in peripheral and enteric neurons, as well as in PC12 cells. A determination of the motile properties of peripherin has been undertaken in PC12 cells during different stages of neurite outgrowth. The results reveal that non-filamentous, non-membrane bound peripherin particles and short peripherin intermediate filaments, termed `squiggles', are transported at high speed throughout PC12 cell bodies, neurites and growth cones. These movements are bi-directional, and the majority require microtubules along with their associated molecular motors, conventional kinesin and cytoplasmic dynein. Our data demonstrate that peripherin particles and squiggles can move as components of a rapid transport system capable of delivering cytoskeletal subunits to the most distal regions of neurites over relatively short time periods.

Key words: Intermediate filaments, Peripherin, Dynein, Kinesin, Cytoskeleton

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