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First published online 20 May 2003
doi: 10.1242/jcs.00496
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Research Article |
1 Department of Molecular Genetics, Research Institute for Microbial Diseases,
Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
2 Department of Integrated Biosciences, Graduate School of Frontier Sciences
Tokyo University, Bldg. FSB-101/601, 5-1-5 Kashiwanoha, Kashiwa, Chiba
277-8562, Japan
Author for correspondence (e-mail:
hnojima{at}biken.osaka-u.ac.jp)
Accepted 18 March 2003
Using a meiosis-specific subtracted cDNA library of Schizosaccharomyces
pombe, we identified meu14+ as a gene whose
expression is upregulated during meiosis. Transcription of
meu14+ is induced abruptly after the cell enters meiosis.
Its transcription is dependent on the meiosis-specific transcription factor
Mei4. In meu14
cells, the segregation and modification of the
SPBs (spindle pole bodies) and microtubule elongation during meiosis II were
aberrant. Meiotic meu14 cells consequently produced a high
frequency of abnormal tetranucleate cells harboring aberrant forespore
membranes and failed to produce asci. In wild-type cells harboring the
integrated meu14+-gfp fusion gene, Meu14-GFP
first appeared inside the nuclear region at prophase II, after which it
accumulated beside the two SPBs at metaphase II. Thereafter, it formed two
ring-shaped structures that surrounded the nucleus at early anaphase II. At
post-anaphase II, it disappeared. Meu14-GFP appears to localize at the border
of the forespore membrane that later develops into spore walls at the end of
sporulation. This was confirmed by coexpressing Spo3-HA, a component of the
forespore membrane, with Meu14-GFP. Taken together, we conclude that
meu14+ is crucial in meiosis in that it participates in
both the nuclear division during meiosis II and the accurate formation of the
forespore membrane.
Key words: Meiosi, Spore formation, Forespore membrane, Microtubule, Spindle pole body, Schizosaccharomyces pombe
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