|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 23 January 2003
doi: 10.1242/jcs.00330
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Departamento de Biología, Edificio de Biológicas, Universidad
Autónoma de Madrid, E-28049 Madrid, Spain
2 Institute of Cell and Molecular Biology, University of Edinburgh, Swann
Building, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland,
UK
* Author for correspondence (e-mail: jose.suja{at}uam.es)
Accepted 18 December 2002
INCENP and aurora-B kinase are two chromosomal passenger proteins that are thought to play key roles in coordinating chromosome segregation with cytokinesis in somatic cells. Here we have analyzed their subcellular distribution, and that of phosphorylated histone H3, and the timing of their relative appearance in mouse spermatocytes during both meiotic divisions. Our results show that in mitotic spermatogonial cells, INCENP and aurora-B show the same pattern of distribution as they do in cultured somatic cells. INCENP labels the synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocentres. Aurora-B first appears at chromocentres in late diplotene before the initial phosphorylation of histone H3. INCENP and aurora-B concentrate at centromeres during diakinesis and appear during metaphase I as T-shaped signals at their inner domains, just below associated sister kinetochores. During late anaphase I both proteins relocalize to the spindle midzone. Both proteins colocalize at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone. We discuss the complex dynamic relocalization of the chromosomal passenger complex during prophase I. Additionally, we suggest that this complex may regulate sister-chromatid centromere cohesion during both meiotic divisions.
Key words: INCENP, Aurora-B kinase, Phosphorylated histone H3, Meiosis, Centromere, Sister-chromatid cohesion
This article has been cited by other articles:
|
|
 |
|
  T. M. Yamamoto, A. L. Lewellyn, and J. L. Maller Regulation of the Aurora B Chromosome Passenger Protein Complex during Oocyte Maturation in Xenopus laevis Mol. Cell. Biol., June 15, 2008; 28(12): 4196 - 4203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kimmins, C. Crosio, N. Kotaja, J. Hirayama, L. Monaco, C. Hoog, M. van Duin, J. A. Gossen, and P. Sassone-Corsi Differential Functions of the Aurora-B and Aurora-C Kinases in Mammalian Spermatogenesis Mol. Endocrinol., March 1, 2007; 21(3): 726 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Cervantes, X. Xi, D. Vermaak, M.-C. Yao, and H. S. Malik The CNA1 Histone of the Ciliate Tetrahymena thermophila Is Essential for Chromosome Segregation in the Germline Micronucleus Mol. Biol. Cell, January 1, 2006; 17(1): 485 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Firooznia, E. Revenkova, and R. Jessberger From the XXVII North American Testis Workshop: The Function of SMC and Other Cohesin Proteins in Meiosis J Androl, January 1, 2005; 26(1): 1 - 10. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kimmins, N. Kotaja, I. Davidson, and P. Sassone-Corsi Testis-specific transcription mechanisms promoting male germ-cell differentiation Reproduction, July 1, 2004; 128(1): 5 - 12. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Parra, A. Viera, R. Gomez, J. Page, R. Benavente, J. L. Santos, J. S. Rufas, and J. A. Suja Involvement of the cohesin Rad21 and SCP3 in monopolar attachment of sister kinetochores during mouse meiosis I J. Cell Sci., March 1, 2004; 117(7): 1221 - 1234. [Abstract] [Full Text] [PDF]
|
|
