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First published online 11 March 2003
doi: 10.1242/jcs.00311

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Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells

Rustem Uzbekov^1,2, Elmira Timirbulatova², Erwan Watrin¹, Fabien Cubizolles¹, David Ogereau¹, Pavel Gulak³, Vincent Legagneux¹, Vladimir Ju. Polyakov⁴, Katherine Le Guellec^1,* and Igor Kireev^2,5,

¹ Groupe Structure Dynamique de la Chromatine, CNRS, UMR 6061, Faculte de Medicine, 35043, Rennes, France
² Cell Cycle Group, Division of Electron Microscopy, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Moscow, Russia
³ Institute of Agricultural Biotechnology, 127550 Moscow, Russia
⁴ Division of Electron Microscopy, A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899, Moscow, Russia
⁵ Department of Cell and Structural Biology, University of Illinois at Urbana-Champaign, Urbana 61801, USA

Author for correspondence (e-mail: ikireev{at}life.uiuc.edu)

Accepted 6 December 2002

Cell cycle dynamics and localization of condensins — multiprotein complexes involved in late stages of mitotic chromosome condensation — were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to ß-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.

Key words: Chromosome condensation, Condensin, Chromatin, Nucleolus

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