spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 1 June 2004
doi: 10.1242/jcs.01149

Journal of Cell Science 117, 2909-2916 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01149v1
117/14/2909    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Furlan, F.
Right arrow Articles by Mondino, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Furlan, F.
Right arrow Articles by Mondino, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

The soluble D2D388-274 fragment of the urokinase receptor inhibits monocyte chemotaxis and integrin-dependent cell adhesion

Federico Furlan1,*, Simone Orlando1,*, Carlo Laudanna2, Massimo Resnati1, Veronica Basso1, Francesco Blasi1,*,{ddagger} and Anna Mondino1,*

1 Department of Molecular Biology and Functional Genomics, San Raffaele Scientific Institute, Milan, 20132, Italy
2 Division of General Pathology, Department of Pathology, University of Verona, Verona, 37129, Italy

{ddagger} Author for correspondence (e-mail: francesco.blasi{at}hsr.it)

Accepted 11 February 2004

We have previously shown that chymotrypsin-cleaved soluble uPAR (D2D388-274) elicits migration of monocytic cells through interaction with FPRL-1, a G protein-coupled receptor that is homologous to the fMLP receptor. Here, we report that D2D388-274 also modulates the ability of monocytes to migrate in response to other chemokines. Pretreatment of monocytes with increasing amounts of D2D388-274 prevents cell migration in response to MCP-1, RANTES and fMLP. We demonstrate that D2D388-274 does not inhibit MCP-1 receptor binding, elicit CCR2 internalization and prevent MCP-1-induced intracellular Ca2+ increase. Thus, CCR2 receptor desensitization cannot account for D2D388-274-mediated inhibition of MCP-1-induced cell migration. Rather, we show that pretreatment of monocytes with D2D388-274 dramatically decreases chemokine-induced integrin-dependent rapid cell adhesion by interacting with FPRL-1. Together, our results indicate that chemokine-dependent cell migration can be regulated not only by homologous and heterologous receptor desensitization, but also by inhibition of integrin-dependent cell adhesion, an important step in cell transmigration.

Key words: Monocytes, Chemoattractants, Cell migration, Cell adhesion


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
V. D'mello, S. Singh, Y. Wu, and R. B. Birge
The Urokinase Plasminogen Activator Receptor Promotes Efferocytosis of Apoptotic Cells
J. Biol. Chem., June 19, 2009; 284(25): 17030 - 17038.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
R. D. Ye, F. Boulay, J. M. Wang, C. Dahlgren, C. Gerard, M. Parmentier, C. N. Serhan, and P. M. Murphy
International Union of Basic and Clinical Pharmacology. LXXIII. Nomenclature for the Formyl Peptide Receptor (FPR) Family
Pharmacol. Rev., June 1, 2009; 61(2): 119 - 161.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
S. Shetty, J. Padijnayayveetil, T. Tucker, D. Stankowska, and S. Idell
The fibrinolytic system and the regulation of lung epithelial cell proteolysis, signaling, and cellular viability
Am J Physiol Lung Cell Mol Physiol, December 1, 2008; 295(6): L967 - L975.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. P. Mazar
Urokinase Plasminogen Activator Receptor Choreographs Multiple Ligand Interactions: Implications for Tumor Progression and Therapy
Clin. Cancer Res., September 15, 2008; 14(18): 5649 - 5655.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
I. Martinez, L. Lombardia, B. Garcia-Barreno, O. Dominguez, and J. A. Melero
Distinct gene subsets are induced at different time points after human respiratory syncytial virus infection of A549 cells
J. Gen. Virol., February 1, 2007; 88(2): 570 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. Selleri, N. Montuori, P. Ricci, V. Visconte, A. Baiano, M. V. Carriero, B. Rotoli, G. Rossi, and P. Ragno
In vivo Activity of the Cleaved Form of Soluble Urokinase Receptor: A New Hematopoietic Stem/Progenitor Cell Mobilizer.
Cancer Res., November 15, 2006; 66(22): 10885 - 10890.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
R. Mazzieri, S. D'Alessio, R. K. Kenmoe, L. Ossowski, and F. Blasi
An Uncleavable uPAR Mutant Allows Dissection of Signaling Pathways in uPA-dependent Cell Migration
Mol. Biol. Cell, January 1, 2006; 17(1): 367 - 378.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Gargiulo, I. Longanesi-Cattani, K. Bifulco, P. Franco, R. Raiola, P. Campiglia, P. Grieco, G. Peluso, M. P. Stoppelli, and M. V. Carriero
Cross-talk between fMLP and Vitronectin Receptors Triggered by Urokinase Receptor-derived SRSRY Peptide
J. Biol. Chem., July 1, 2005; 280(26): 25225 - 25232.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004