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First published online 27 July 2004
doi: 10.1242/jcs.01242

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Survivin dynamics increases at centromeres during G2/M phase transition and is regulated by microtubule-attachment and Aurora B kinase activity

Victoria A. Beardmore^1,*,, Leena J. Ahonen^2,*, Gary J. Gorbsky² and Marko J. Kallio^1,3,

¹ University of Oklahoma Health Sciences Center, Department of Cell Biology, 940 Stanton L. Young Boulevard, Oklahoma City, OK 73104, USA
² Oklahoma Medical Research Foundation, Molecular, Cell and Developmental Biology Research Program, 825 N.E. 13th Street, Oklahoma City, OK 73104, USA
³ VTT-Medical Biotechnology and University of Turku, P.O. Box 106, 20521 Turku, Finland

Author for correspondence (e-mail: marko.kallio{at}vtt.fi)

Accepted 1 April 2004

The inhibitor of apoptosis protein survivin is implicated in two key biological events: in the control of cell proliferation and in the regulation of cell lifespan. Although the details of mitotic roles of survivin are unclear, the protein appears to modulate microtubule function and might participate in regulating the spindle checkpoint. Survivin physically associates with Aurora B, a serine-threonine kinase involved in microtubule attachment to centromeres and regulation of chromosome segregation. Here we have examined the dynamics and localization of a survivin-GFP chimera using high-resolution fluorescence microscopy and photobleaching. Survivin forms a bi-partite structure at the inner centromere that undergoes significant stretching during mitosis. Photobleaching experiments revealed marked changes in rates of survivin turnover at centromeres. These were regulated by stage of the cell cycle, microtubule attachment, and Aurora B kinase activity. We hypothesize that changes in the turnover of survivin at centromeres influence the stability of kinetochore-microtubule attachment and signaling of the spindle checkpoint.

Key words: Survivin, Microtubules, Mitosis, Spindle checkpoint, FRAP

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