QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 17 August 2004
doi: 10.1242/jcs.01310

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.01310v1 117/19/4481    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sigle, R. O. Articles by Carter, W. G. Search for Related Content PubMed PubMed Citation Articles by Sigle, R. O. Articles by Carter, W. G. Social Bookmarking                         What's this?

Globular domains 4/5 of the laminin 3 chain mediate deposition of precursor laminin 5

Randy O. Sigle¹, Susana G. Gil¹, Mallar Bhattacharya², Maureen C. Ryan^1,*, Tai-Mei Yang¹, Tod A. Brown¹, Ariel Boutaud⁴, Yuko Miyashita², John Olerud² and William G. Carter^1,3,

¹ Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue, Seattle, WA 98109, USA
² Department of Medicince (Dermatology), University of Washington, 1959 N.E. Pacific St, Seattle, WA 98195, USA
³ Department of Pathobiology, University of Washington, 1959 N.E. Pacific St, Seattle, WA 98195, USA
⁴ BioStratum Incorporated, 4620 Creekstone Drive, Suite 200, Durham, NC 27703, USA

Author for correspondence (e-mail: wcarter{at}fhcrc.org)

Accepted 12 May 2004

In epidermal wounds, precursor laminin 5 (3ß32) is deposited in the provisional basement membrane (PBM) before other BM components. Precursor laminin 5 contains G4/5 globular domains at the carboxyl terminus of the 3 chain. Here, the function of G4/5 was evaluated in deposition of laminin 5. Soluble laminin 5, secreted by keratinocytes in culture, is cleaved by an endogenous protease releasing G4/5. Thrombin, a serum protease, cleaves G4/5 indistinguishably from endogenous protease. Soluble human precursor laminin 5, but not cleaved laminin 5, was bound and deposited by mouse keratinocytes null for mouse 3 chain (3–/– MKs). The deposition rescued adhesion and spreading and survival. In a model for PBM assembly, precursor laminin 5 was deposited along fibronectin fibrils at the junction between co-cultures of keratinocytes and fibroblasts. In both models, the deposition of precursor laminin 5 was inhibited by removal of G4/5 with thrombin. To confirm that G4/5 participates in deposition, the human LAMA3A gene was modified to produce 3 chains either without or with G4/5 that cannot be cleaved. Both precleaved and noncleavable 3 isoforms were expressed in 3–/– MKs, where they deposited sufficiently to rescue adhesion via integrins 3ß1 and 6ß4. Despite this similarity, noncleavable laminin 5 was at least threefold more efficiently deposited than precleaved isoform. We conclude that the G4/5 domain in the 3 chain facilitates deposition of precursor laminin 5 into the PBM in epidermal wounds.

Key words: Laminin 5, Adhesion, Basement membrane assembly, Extracellular matrix

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				E. Araki, Y. Momota, T. Togo, M. Tanioka, K. Hozumi, M. Nomizu, Y. Miyachi, and A. Utani Clustering of Syndecan-4 and Integrin {beta}1 by Laminin {alpha}3 Chain-derived Peptide Promotes Keratinocyte Migration Mol. Biol. Cell, July 1, 2009; 20(13): 3012 - 3024. [Abstract] [Full Text] [PDF]

				M. L. Usui, J. N. Mansbridge, W. G. Carter, M. Fujita, and J. E. Olerud Keratinocyte Migration, Proliferation, and Differentiation in Chronic Ulcers From Patients With Diabetes and Normal Wounds J. Histochem. Cytochem., July 1, 2008; 56(7): 687 - 696. [Abstract] [Full Text] [PDF]

				M. Tran, P. Rousselle, P. Nokelainen, S. Tallapragada, N. T. Nguyen, E. F. Fincher, and M. P. Marinkovich Targeting a Tumor-Specific Laminin Domain Critical for Human Carcinogenesis Cancer Res., April 15, 2008; 68(8): 2885 - 2894. [Abstract] [Full Text] [PDF]

				T. Ogawa, Y. Tsubota, J. Hashimoto, Y. Kariya, and K. Miyazaki The Short Arm of Laminin {gamma}2 Chain of Laminin-5 (Laminin-332) Binds Syndecan-1 and Regulates Cellular Adhesion and Migration by Suppressing Phosphorylation of Integrin beta4 Chain Mol. Biol. Cell, May 1, 2007; 18(5): 1621 - 1633. [Abstract] [Full Text] [PDF]

				P S Tsai, J E Evans, K M Green, R M Sullivan, D A Schaumberg, S M Richards, M R Dana, and D A Sullivan Proteomic analysis of human meibomian gland secretions. Br J Ophthalmol, March 1, 2006; 90(3): 372 - 377. [Abstract] [Full Text] [PDF]

				E. G. Harper, S. M. Alvares, and W. G. Carter Wounding activates p38 map kinase and activation transcription factor 3 in leading keratinocytes J. Cell Sci., August 1, 2005; 118(15): 3471 - 3485. [Abstract] [Full Text] [PDF]

				Y. Tsubota, C. Yasuda, Y. Kariya, T. Ogawa, T. Hirosaki, H. Mizushima, and K. Miyazaki Regulation of Biological Activity and Matrix Assembly of Laminin-5 by COOH-terminal, LG4-5 Domain of {alpha}3 Chain J. Biol. Chem., April 15, 2005; 280(15): 14370 - 14377. [Abstract] [Full Text] [PDF]