|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 2 December 2003
doi: 10.1242/jcs.00845
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
ko Ili
1,*
i2evi5
1 Department of Stomatology, 2 Medicine, and 10 Anatomy, and the 7 Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California 94143, USA
3 Department of Anatomy and Organ Technology, and 9 Institute of Organ Transplants, Reconstructive Medicine and Tissue Engineering, Shinshu University School of Medicine, Matsumoto 390-8621, Japan
4 Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA
5 Biochemistry Department, Cytokinetics Inc., South San Francisco, California 94080, USA
6 Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland, Oregon 97201, USA
8 Department of Microbiology, School of Medicine Chiba University, Chiba 260, Japan
* Author for correspondence (e-mail: ilic{at}itsa.ucsf.edu)
Accepted 27 August 2003
Targeted disruption of the focal adhesion kinase (FAK) gene in mice is lethal at embryonic day 8.5 (E8.5). Vascular defects in FAK-/- mice result from the inability of FAK-deficient endothelial cells to organize themselves into vascular network. We found that, although fibronectin (FN) levels were similar, its organization was less fibrillar in both FAK-/- endothelial cells and mesoderm of E8.5 FAK-/- embryos, as well as in mouse embryonic fibroblasts isolated from mutant embryos. FAK catalytic activity, proline-rich domains, and location in focal contacts were all required for proper allocation and patterning of FN matrix. Cells lacking FAK in focal adhesions fail to translocate supramolecular complexes of integrin-bound FN and focal adhesion proteins along actin filaments to form mature fibrillar adhesions. Taken together, our data suggest that proper FN allocation and organization are dependent on FAK-mediated remodeling of focal adhesions.
Key words: FAK, Fibronectin matrix allocation, Fibrillar adhesions, Focal adhesion dynamics, Motility
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Huveneers and E. H. J. Danen Adhesion signaling - crosstalk between integrins, Src and Rho J. Cell Sci., April 15, 2009; 122(8): 1059 - 1069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Frontini, C. O'Neil, C. Sawyez, B. M.C. Chan, M. W. Huff, and J. G. Pickering Lipid Incorporation Inhibits Src-Dependent Assembly of Fibronectin and Type I Collagen by Vascular Smooth Muscle Cells Circ. Res., April 10, 2009; 104(7): 832 - 841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Sayers, L. J. Sundberg-Smith, M. Rojas, H. Hayasaka, J. T. Parsons, C. P. Mack, and J. M. Taylor FRNK Expression Promotes Smooth Muscle Cell Maturation During Vascular Development and After Vascular Injury Arterioscler Thromb Vasc Biol, December 1, 2008; 28(12): 2115 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kinsey, M. R. Williamson, S. Chaudhry, K. T. Mellody, A. McGovern, S. Takahashi, C. A. Shuttleworth, and C. M. Kielty Fibrillin-1 microfibril deposition is dependent on fibronectin assembly J. Cell Sci., August 15, 2008; 121(16): 2696 - 2704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Park, C. Will, B. Martin, L. Gullotti, N. Friedrichs, R. Buettner, H. Schneider, S. Ludwig, and V. Wixler Deficiency in the LIM-only protein FHL2 impairs assembly of extracellular matrix proteins FASEB J, July 1, 2008; 22(7): 2508 - 2520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Schlunck, H. Han, T. Wecker, D. Kampik, T. Meyer-ter-Vehn, and F. Grehn Substrate Rigidity Modulates Cell Matrix Interactions and Protein Expression in Human Trabecular Meshwork Cells Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 262 - 269. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Honda, T. Sakaguchi, K. Sakai, C. Schmedt, A. Ramirez, J. L. Jorcano, A. Tarakhovsky, H. Kamisoyama, and T. Sakai Epidermal hyperplasia and papillomatosis in mice with a keratinocyte-restricted deletion of csk Carcinogenesis, October 1, 2007; 28(10): 2074 - 2081. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Thomas, M. Peters-Golden, E. S. White, V. J. Thannickal, and B. B. Moore PGE2 inhibition of TGF-beta1-induced myofibroblast differentiation is Smad-independent but involves cell shape and adhesion-dependent signaling Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L417 - L428. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Papp, M. P. Fadel, H. Kim, C. A. McCulloch, and M. Opas Calreticulin Affects Fibronectin-based Cell-Substratum Adhesion via the Regulation of c-Src Activity J. Biol. Chem., June 1, 2007; 282(22): 16585 - 16598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Ilic, M. Mao-Qiang, D. Crumrine, G. Dolganov, N. Larocque, P. Xu, M. Demerjian, B. E. Brown, S.-T. Lim, V. Ossovskaya, et al. Focal Adhesion Kinase Controls pH-Dependent Epidermal Barrier Homeostasis by Regulating Actin-Directed Na+/H+ Exchanger 1 Plasma Membrane Localization Am. J. Pathol., June 1, 2007; 170(6): 2055 - 2067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Basson, M. A. Sanders, R. Gomez, J. Hatfield, R. VanderHeide, V. Thamilselvan, J. Zhang, and M. F. Walsh Focal adhesion kinase protein levels in gut epithelial motility. Am J Physiol Gastrointest Liver Physiol, September 1, 2006; 291(3): G491 - G499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Winters, B. K. M. Raj, E. E. Robinson, R. A. Foty, and S. A. Corbett Three-dimensional Culture Regulates Raf-1 Expression to Modulate Fibronectin Matrix Assembly Mol. Biol. Cell, August 1, 2006; 17(8): 3386 - 3396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Greenberg, A. M. Bernstein, M. Benezra, I. H. Gelman, L. Taliana, and S. K. Masur FAK-dependent regulation of myofibroblast differentiation FASEB J, May 1, 2006; 20(7): 1006 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Lagana, J. G. Goetz, P. Cheung, A. Raz, J. W. Dennis, and I. R. Nabi Galectin Binding to Mgat5-Modified N-Glycans Regulates Fibronectin Matrix Remodeling in Tumor Cells Mol. Cell. Biol., April 15, 2006; 26(8): 3181 - 3193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Tang, F. Mack, V. H. Haase, M. C. Simon, and R. S. Johnson pVHL Function Is Essential for Endothelial Extracellular Matrix Deposition. Mol. Cell. Biol., April 1, 2006; 26(7): 2519 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Romer, K. G. Birukov, and J. G.N. Garcia Focal Adhesions: Paradigm for a Signaling Nexus Circ. Res., March 17, 2006; 98(5): 606 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Kragtorp and J. R. Miller Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development Development, February 15, 2006; 133(4): 685 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Mao and J. E. Schwarzbauer Stimulatory effects of a three-dimensional microenvironment on cell-mediated fibronectin fibrillogenesis J. Cell Sci., October 1, 2005; 118(19): 4427 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. van Nimwegen, S. Verkoeijen, L. van Buren, D. Burg, and B. van de Water Requirement for Focal Adhesion Kinase in the Early Phase of Mammary Adenocarcinoma Lung Metastasis Formation Cancer Res., June 1, 2005; 65(11): 4698 - 4706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Agerer, S. Lux, A. Michel, M. Rohde, K. Ohlsen, and C. R. Hauck Cellular invasion by Staphylococcus aureus reveals a functional link between focal adhesion kinase and cortactin in integrin-mediated internalisation J. Cell Sci., May 15, 2005; 118(10): 2189 - 2200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grabbe, C. G. Zervas, T. Hunter, N. H. Brown, and R. H. Palmer Focal adhesion kinase is not required for integrin function or viability in Drosophila Development, December 1, 2004; 131(23): 5795 - 5805. [Abstract] [Full Text] [PDF]
|
|
