spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 25 August 2004
doi: 10.1242/jcs.01333

Journal of Cell Science 117, 4739-4748 (2004)
Published by The Company of Biologists 2004
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01333v1
117/20/4739    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Xin, X.
Right arrow Articles by Mains, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Xin, X.
Right arrow Articles by Mains, R. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Cdk5 and Trio modulate endocrine cell exocytosis

Xiaonan Xin1, Francesco Ferraro1, Nils Bäck2, Betty A. Eipper1 and Richard E. Mains1,*

1 Department of Neuroscience, University of Connecticut Health Center, 263 Farmington Avenue, Farmington, CT 06030, USA
2 Department of Anatomy, Institute of Biomedicine, University of Helsinki, Haartmaninkatu 8, Biomedicum, FI-00014 Helsinki, Finland

* Author for correspondence (e-mail: mains{at}uchc.edu)

Accepted 27 May 2004

Hormone secretion by pituitary cells is decreased by roscovitine, an inhibitor of cyclin-dependent kinase 5 (Cdk5). Roscovitine treatment reorganizes cortical actin and ultrastructural analysis demonstrates that roscovitine limits the ability of secretory granules to approach the plasma membrane or one another. Trio, a multifunctional RhoGEF expressed in pituitary cells, interacts with peptidylglycine {alpha}-amidating monooxygenase, a secretory granule membrane protein known to affect the actin cytoskeleton. Roscovitine inhibits the ability of Trio to activate Rac, and peptides corresponding to the Cdk5 consensus sites in Trio are phosphorylated by Cdk5. Together, these data suggest that control of the cortical actin cytoskeleton, long known to modulate hormone exocytosis and subsequent endocytosis, involves Cdk5-mediated activation of Trio.

Key words: GDP/GTP exchange factor (GEF), Cytoskeleton, Actin, Peptidylglycine {alpha}-amidating monooxygenase (PAM), RhoGTPase, P21-activated kinase (PAK)


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 EndocrinologyHome page
H. Lin, M.-C. Chen, and C.-T. Ku
Cyclin-Dependent Kinase 5 Regulates Steroidogenic Acute Regulatory Protein and Androgen Production in Mouse Leydig Cells
Endocrinology, January 1, 2009; 150(1): 396 - 403.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
X. Xin, Y. Wang, X.-m. Ma, P. Rompolas, H. T. Keutmann, R. E. Mains, and B. A. Eipper
Regulation of Kalirin by Cdk5
J. Cell Sci., August 1, 2008; 121(15): 2601 - 2611.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Ferraro, X.-M. Ma, J. A. Sobota, B. A. Eipper, and R. E. Mains
Kalirin/Trio Rho Guanine Nucleotide Exchange Factors Regulate a Novel Step in Secretory Granule Maturation
Mol. Biol. Cell, December 1, 2007; 18(12): 4813 - 4825.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
F. Causeret, T. Jacobs, M. Terao, O. Heath, M. Hoshino, and M. Nikolic
Neurabin-I Is Phosphorylated by Cdk5: Implications for Neuronal Morphogenesis and Cortical Migration
Mol. Biol. Cell, November 1, 2007; 18(11): 4327 - 4342.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Backer, M. Hidalgo-Sanchez, N. Offner, E. Portales-Casamar, A. Debant, P. Fort, C. Gauthier-Rouviere, and E. Bloch-Gallego
Trio Controls the Mature Organization of Neuronal Clusters in the Hindbrain
J. Neurosci., September 26, 2007; 27(39): 10323 - 10332.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Taniguchi, M. Taoka, M. Itakura, A. Asada, T. Saito, M. Kinoshita, M. Takahashi, T. Isobe, and S.-i. Hisanaga
Phosphorylation of Adult Type Sept5 (CDCrel-1) by Cyclin-dependent Kinase 5 Inhibits Interaction with Syntaxin-1
J. Biol. Chem., March 16, 2007; 282(11): 7869 - 7876.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. K. Y. Fu, F. C. F. Ip, W.-Y. Fu, J. Cheung, J. H. Wang, W.-H. Yung, and N. Y. Ip
Aberrant motor axon projection, acetylcholine receptor clustering, and neurotransmission in cyclin-dependent kinase 5 null mice
PNAS, October 18, 2005; 102(42): 15224 - 15230.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R. Steven, L. Zhang, J. Culotti, and T. Pawson
The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans
Genes & Dev., September 1, 2005; 19(17): 2016 - 2029.
[Abstract] [Full Text] [PDF]

Home page
 NeuroscientistHome page
C. A. Rabiner, R. E. Mains, and B. A. Eipper
Kalirin: A Dual Rho Guanine Nucleotide Exchange Factor That Is So Much More Than the Sum of Its Many Parts
Neuroscientist, April 1, 2005; 11(2): 148 - 160.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Rosales, J. D. Ernst, J. Hallows, and K.-Y. Lee
GTP-dependent Secretion from Neutrophils Is Regulated by Cdk5
J. Biol. Chem., December 24, 2004; 279(52): 53932 - 53936.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004