QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 14 September 2004
doi: 10.1242/jcs.01372

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.01372v1 117/21/4959    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kvam, E. Articles by Goldfarb, D. S. Search for Related Content PubMed PubMed Citation Articles by Kvam, E. Articles by Goldfarb, D. S. Social Bookmarking                         What's this?

Nvj1p is the outer-nuclear-membrane receptor for oxysterol-binding protein homolog Osh1p in Saccharomyces cerevisiae

Department of Biology, University of Rochester, 436 Hutchison Hall, Rochester, New York, NY 14627, USA

^* Author for correspondence (e-mail: dasg{at}mail.rochester.edu)

Accepted 21 June 2004

OSH1 belongs to a seven-member gene family in yeast that is related to mammalian oxysterol-binding protein (OSBP). Here, we investigate the targeting of Osh1p to nucleus-vacuole (NV) junctions in Saccharomyces cerevisiae. NV junctions are interorganelle interfaces mediated by Nvj1p in the nuclear envelope and Vac8p on the vacuole membrane. Together, Nvj1p and Vac8p form Velcro-like patches through which teardrop-like portions of the nucleus are pinched off into the vacuolar lumen and degraded by a process termed piecemeal microautophagy of the nucleus (PMN). Osh1p is targeted to NV junctions proportional to NVJ1 expression through a physical association with Nvj1p. NV junctions per se are not required for this targeting because Osh1p colocalizes with Nvj1p in the absence of Vac8p. NV-junction-associated Osh1p is also a substrate for PMN degradation. Although OSH1 is not required for NV-junction formation or PMN, PMN is defective in cells lacking the yeast OSBP family (Osh1p to Osh7p). By contrast, the vesicular targeting of aminopeptidase I to the vacuole by macroautophagy is not dependent on the Osh protein family. We conclude the formation of nuclear PMN vesicles requires the overlapping activities of Osh1p and other Osh family members.

Key words: Nucleus-vacuole junction, Oxysterol-binding protein, Autophagy

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

Related articles in JCS:

Nuclear Velcro

JCS 2004 117: 2104. [Full Text]  

This article has been cited by other articles:

				R. Krick, Y. Muehe, T. Prick, S. Bremer, P. Schlotterhose, E.-L. Eskelinen, J. Millen, D. S. Goldfarb, and M. Thumm Piecemeal Microautophagy of the Nucleus Requires the Core Macroautophagy Genes Mol. Biol. Cell, October 1, 2008; 19(10): 4492 - 4505. [Abstract] [Full Text] [PDF]

				E. Kvam and D. S. Goldfarb Structure and function of nucleus-vacuole junctions: outer-nuclear-membrane targeting of Nvj1p and a role in tryptophan uptake J. Cell Sci., September 1, 2006; 119(17): 3622 - 3633. [Abstract] [Full Text] [PDF]

				E. Kvam, K. Gable, T. M. Dunn, and D. S. Goldfarb Targeting of Tsc13p to Nucleus-Vacuole Junctions: A Role for Very-Long-Chain Fatty Acids in the Biogenesis of Microautophagic Vesicles Mol. Biol. Cell, September 1, 2005; 16(9): 3987 - 3998. [Abstract] [Full Text] [PDF]