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First published online 23 November 2004
doi: 10.1242/jcs.01566

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TNF induces sequential activation of Cdc42- and p38/p53-dependent pathways that antagonistically regulate filopodia formation

Centre de Recherche en Biochimie Macromoléculaire, CNRS FRE 2593, IFR 24, 1919 route de Mende, 34293 Montpellier CEDEX 5, France

^* Author for correspondence (e-mail: pierre.roux{at}crbm.cnrs.fr)

Accepted 27 September 2004

Cell migration is an essential function in various physiological processes, including tissue repair and tumour invasion. Repair of tissue damage requires the recruitment of fibroblasts to sites of tissue injury, which is mediated in part by the cytokine tumour necrosis factor (TNF). As dynamic rearrangements of actin cytoskeleton control cell locomotion, this implicates that TNF is a potent coordinator of cellular actin changes. We have investigated the role of TNF in regulating the cortical actin-containing structures essential for cell locomotion called filopodia. Kinetic analysis of TNF-treated mouse embryonic fibroblasts (MEFs) revealed a dual effect on filopodia formation: a rapid and transient induction mediated by Cdc42 GTPase that is then counteracted by a subsequent sustained inhibition requiring activation of the mitogen-activated protein kinase p38 but not Cdc42 activity. This inhibition also involves the tumour suppressor p53, given that it is activated in response to TNF following the same time course as the decrease of filopodia formation. This functional activation of p53, measured by transcription induction of its target p21^WAF1(p21), is also associated with p38 kinase-dependent phosphorylation of p53 at serine 18. Furthermore, TNF did not inhibit filopodia formation in MEFs treated with the transcription inhibitor actinomycin D, in p53-deficient MEFs, or MEFs expressing p53 mutants H273 or H175, which supports a role for the transcriptional activity of p53 in mediating TNF-dependent filopodia inhibition. Our data delineate a novel inhibitory pathway in which TNF prevents filopodia formation and cell migration through the activation of the mitogen-activated protein kinase (MAPK) p38, which in turn activates p53. This shows that TNF on its own initiates antagonistic signals that modulate events linked to cell migration.

Key words: p53, TNF, Filopodia, p38 kinase, Cdc42, Phosphorylation

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