|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 17 February 2004
doi: 10.1242/jcs.00950
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester, M13 9PT, UK
Author for correspondence (e-mail: lowe{at}man.ac.uk)
Accepted 22 October 2003
We report the caspase-dependent cleavage of two Golgi-associated transport factors during apoptosis. The tethering factor giantin is rapidly cleaved both in vitro and in vivo at a conserved site, to generate a stable membrane-anchored domain and a soluble domain that is subject to further caspase-dependent cleavage. The t-SNARE syntaxin 5 is also cleaved rapidly, resulting in the separation of the catalytic membrane-proximal domain from an N-terminal regulatory domain. Cleavage of giantin and syntaxin 5 is accompanied by a cessation of vesicular transport between the ER and the Golgi complex, which first manifests itself as a block in ER exit. The contribution that such an inhibition of trafficking may make towards the generation of an apoptotic phenotype is discussed.
Key words: Golgi apparatus, Apoptosis, SNARE, Vesicle, Caspase
This article has been cited by other articles:
|
|
 |
|
  K.-H. Sun, Y. de Pablo, F. Vincent, E. O. Johnson, A. K. Chavers, and K. Shah Novel Genetic Tools Reveal Cdk5's Major Role in Golgi Fragmentation in Alzheimer's Disease Mol. Biol. Cell, July 1, 2008; 19(7): 3052 - 3069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Sbodio and C. E. Machamer Identification of a Redox-sensitive Cysteine in GCP60 That Regulates Its Interaction with Golgin-160 J. Biol. Chem., October 12, 2007; 282(41): 29874 - 29881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chandraratna, N. Lawrence, D. P. Welchman, and B. Sanson An in vivo model of apoptosis: linking cell behaviours and caspase substrates in embryos lacking DIAP1 J. Cell Sci., August 1, 2007; 120(15): 2594 - 2608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Sbodio, S. W. Hicks, D. Simon, and C. E. Machamer GCP60 Preferentially Interacts with a Caspase-generated Golgin-160 Fragment J. Biol. Chem., September 22, 2006; 281(38): 27924 - 27931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Moss, V. M. Betin, S. D. Malesinski, and J. D. Lane A novel role for microtubules in apoptotic chromatin dynamics and cellular fragmentation J. Cell Sci., June 1, 2006; 119(11): 2362 - 2374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. G. Nacak, K. Leptien, D. Fellner, H. G. Augustin, and J. Kroll The BTB-kelch Protein LZTR-1 Is a Novel Golgi Protein That Is Degraded upon Induction of Apoptosis J. Biol. Chem., February 24, 2006; 281(8): 5065 - 5071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Abu-Zant, M. Santic, M. Molmeret, S. Jones, J. Helbig, and Y. Abu Kwaik Incomplete Activation of Macrophage Apoptosis during Intracellular Replication of Legionella pneumophila Infect. Immun., September 1, 2005; 73(9): 5339 - 5349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Lane, V. J. Allan, and P. G. Woodman Active relocation of chromatin and endoplasmic reticulum into blebs in late apoptotic cells J. Cell Sci., September 1, 2005; 118(17): 4059 - 4071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Maag, M. Mancini, A. Rosen, and C. E. Machamer Caspase-resistant Golgin-160 Disrupts Apoptosis Induced by Secretory Pathway Stress and Ligation of Death Receptors Mol. Biol. Cell, June 1, 2005; 16(6): 3019 - 3027. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Liazoghli, S. Perreault, K. D. Micheva, M. Desjardins, and N. Leclerc Fragmentation of the Golgi Apparatus Induced by the Overexpression of Wild-Type and Mutant Human Tau Forms in Neurons Am. J. Pathol., May 1, 2005; 166(5): 1499 - 1514. [Abstract] [Full Text] [PDF]
|
|
