QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online 3 May 2005
doi: 10.1242/jcs.02341

This Article Figures Only Full Text Full Text (PDF) All Versions of this Article: jcs.02341v1 118/10/2295    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jordan, T. Articles by DiMario, J. X. Search for Related Content PubMed PubMed Citation Articles by Jordan, T. Articles by DiMario, J. X. Social Bookmarking                         What's this?

Regulation of skeletal muscle fiber type and slow myosin heavy chain 2 gene expression by inositol trisphosphate receptor 1

Department of Cell Biology and Anatomy, Chicago Medical School, 3333 Green Bay Road, North Chicago, Illinois 60064, USA

^* Author for correspondence (e-mail: joseph.dimario{at}rosalindfranklin.edu)

Accepted 21 February 2005

Innervation-dependent signaling cascades that control activation of downstream transcription factors regulate expression of skeletal muscle fiber type-specific genes. Many of the innervation-regulated signaling cascades in skeletal muscle are dependent on intracellular calcium and the mechanisms by which calcium is released from the sarcoplasmic reticulum (SR). We report that the inositol trisphosphate receptor 1 (IP3R1), responsible for calcium release from the SR as a slow wave, was more abundant in fast contracting compared to slow contracting avian muscle fibers. Furthermore, inhibition of IP3R1 activity by 2-aminoethoxydiphenylborate (2-APB) and xestospongin D induced a fiber type transition and expression of the slow myosin heavy chain 2 (slow MyHC2) gene in innervated fast muscle fibers. Activation of the slow MyHC2 promoter by IP3R1 inhibition was accompanied by a reduction in protein kinase C activity. In addition, inhibition of IP3R1 activity resulted in a reduction of nuclear factor of activated T cells (NFAT)-dependent transcription and nuclear localization, indicating that IP3R1 activity regulated NFAT transcription factor activity in skeletal muscle fibers. Myocyte enhancer factor 2 (MEF2)-dependent transcriptional activity was increased by innervation, but unaffected by IP3R1 activity. The results indicate that IP3R1 activity regulates muscle fiber type-specific gene expression in innervated muscle fibers.

Key words: Myosin heavy chain, IP3R1, Calcium, Avian, Fiber type, NFAT, MEF2

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Nedachi, H. Fujita, and M. Kanzaki Contractile C2C12 myotube model for studying exercise-inducible responses in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1191 - E1204. [Abstract] [Full Text] [PDF]

				H. Shi, J. M. Scheffler, J. M. Pleitner, C. Zeng, S. Park, K. M. Hannon, A. L. Grant, and D. E. Gerrard Modulation of skeletal muscle fiber type by mitogen-activated protein kinase signaling FASEB J, August 1, 2008; 22(8): 2990 - 3000. [Abstract] [Full Text] [PDF]

				J. A. Valdes, E. Gaggero, J. Hidalgo, N. Leal, E. Jaimovich, and M. A. Carrasco NFAT activation by membrane potential follows a calcium pathway distinct from other activity-related transcription factors in skeletal muscle cells Am J Physiol Cell Physiol, March 1, 2008; 294(3): C715 - C725. [Abstract] [Full Text] [PDF]

				G. Mutungi The expression of NFATc1 in adult rat skeletal muscle fibres Exp Physiol, March 1, 2008; 93(3): 399 - 406. [Abstract] [Full Text] [PDF]

				Y. Hinits and S. M. Hughes Mef2s are required for thick filament formation in nascent muscle fibres Development, July 1, 2007; 134(13): 2511 - 2519. [Abstract] [Full Text] [PDF]