spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 12 April 2005
doi: 10.1242/jcs.02317

Journal of Cell Science 118, 1899-1910 (2005)
Published by The Company of Biologists 2005
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02317v1
118/9/1899    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bosgraaf, L.
Right arrow Articles by van Haastert, P. J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bosgraaf, L.
Right arrow Articles by van Haastert, P. J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium

Leonard Bosgraaf1, Arjen Waijer1, Ruchira Engel2, Antonie J. W. G. Visser2, Deborah Wessels3, David Soll3 and Peter J. M. van Haastert1,*

1 Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
2 MicroSpectroscopy Centre, Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands
3 W. M. Keck Dynamic Image Analysis Facility, Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, USA

* Author for correspondence (e-mail: haastert{at}chem.rug.nl)

Accepted 10 February 2005

The regulation of cell polarity plays an important role in chemotaxis. Previously, two proteins termed GbpC and GbpD were identified in Dictyostelium, which contain RasGEF and cyclic nucleotide binding domains. Here we show that gbpC-null cells display strongly reduced chemotaxis, because they are unable to polarise effectively in a chemotactic gradient. However, gbpD-null mutants exhibit the opposite phenotype: cells display improved chemotaxis and appear hyperpolar, because cells make very few lateral pseudopodia, whereas the leading edge is continuously remodelled. Overexpression of GbpD protein results in severely reduced chemotaxis. Cells extend many bifurcated and lateral pseudopodia, resulting in the absence of a leading edge. Furthermore, cells are flat and adhesive owing to an increased number of substrate-attached pseudopodia. This GbpD phenotype is not dependent on intracellular cGMP or cAMP, like its mammalian homolog PDZ-GEF. Previously we showed that GbpC is a high-affinity cGMP-binding protein that acts via myosin II. We conclude that cGMP activates GbpC, mediating the chemoattractant-induced establishment of cell polarity through myosin. GbpD induces the formation of substrate-attached pseudopodia, resulting in increased attachment and suppression of polarity.

Key words: Chemotaxis, Polarity, cGMP, PDZ-GEF


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

RasGEFs show Dicty the way

JCS 2005 118: 902. [Full Text]  



This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
K. Parkinson, P. Bolourani, D. Traynor, N. L. Aldren, R. R. Kay, G. Weeks, and C. R. L. Thompson
Regulation of Rap1 activity is required for differential adhesion, cell-type patterning and morphogenesis in Dictyostelium
J. Cell Sci., February 1, 2009; 122(3): 335 - 344.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. N. van Egmond, A. Kortholt, K. Plak, L. Bosgraaf, S. Bosgraaf, I. Keizer-Gunnink, and P. J. M. van Haastert
Intramolecular Activation Mechanism of the Dictyostelium LRRK2 Homolog Roco Protein GbpC
J. Biol. Chem., October 31, 2008; 283(44): 30412 - 30420.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Mondal, D. Bakthavatsalam, P. Steimle, B. Gassen, F. Rivero, and A. A. Noegel
Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions
J. Cell Biol., October 20, 2008; 181(5): 747 - 760.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
I. Marin, W. N. van Egmond, and P. J. M. van Haastert
The Roco protein family: a functional perspective
FASEB J, September 1, 2008; 22(9): 3103 - 3110.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Kortholt, J. S. King, I. Keizer-Gunnink, A. J. Harwood, and P. J.M. Van Haastert
Phospholipase C Regulation of Phosphatidylinositol 3,4,5-trisphosphate-mediated Chemotaxis
Mol. Biol. Cell, December 1, 2007; 18(12): 4772 - 4779.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. J. Jeon, D.-J. Lee, S. Merlot, G. Weeks, and R. A. Firtel
Rap1 controls cell adhesion and cell motility through the regulation of myosin II
J. Cell Biol., March 26, 2007; 176(7): 1021 - 1033.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kortholt, H. Rehmann, H. Kae, L. Bosgraaf, I. Keizer-Gunnink, G. Weeks, A. Wittinghofer, and P. J. M. Van Haastert
Characterization of the GbpD-activated Rap1 Pathway Regulating Adhesion and Cell Polarity in Dictyostelium discoideum
J. Biol. Chem., August 18, 2006; 281(33): 23367 - 23376.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2005