spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 28 February 2006
doi: 10.1242/jcs.02820

Journal of Cell Science 119, 1130-1143 (2006)
Published by The Company of Biologists 2006
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.02820v1
119/6/1130    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Clemente-Blanco, A.
Right arrow Articles by Correa-Bordes, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Clemente-Blanco, A.
Right arrow Articles by Correa-Bordes, J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

The Cdc14p phosphatase affects late cell-cycle events and morphogenesis in Candida albicans

Andrés Clemente-Blanco1, Alberto González-Novo2, Félix Machín3, David Caballero-Lima1, Luis Aragón3, Miguel Sánchez2, Carlos R. Vázquez de Aldana2, Javier Jiménez2 and Jaime Correa-Bordes1,*

1 Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, Avda Elvas SN, 06071, Badajoz, Spain
2 Instituto de Microbiología-Bioquímica, Departamento de Microbiología y Genética, CSIC/Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain
3 Cell Cycle Group, Clinical Sciences Centre, Medical Research Council, Imperial College London, W12 0NN, UK

* Author for correspondence (e-mail: jcorrea{at}unex.es)

Accepted 2 December 2005

We have characterized the CDC14 gene, which encodes a dual-specificity protein phosphatase in Candida albicans, and demonstrated that its deletion results in defects in cell separation, mitotic exit and morphogenesis. The C. albicans cdc14{Delta} mutants formed large aggregates of cells that resembled those found in ace2-null strains. In cdc14{Delta} cells, expression of Ace2p target genes was reduced and Ace2p did not accumulate specifically in daughter nuclei. Taken together, these results imply that Cdc14p is required for the activation and daughter-specific nuclear accumulation of Ace2p. Consistent with a role in cell separation, Cdc14p was targeted to the septum region during the M-G1 transition in yeast-form cells. Interestingly, hypha-inducing signals abolished the translocation of Cdc14p to the division plate, and this regulation depended on the cyclin Hgc1p, since hgc1{Delta} mutants were able to accumulate Cdc14p in the septum region of the germ tubes. In addition to its role in cytokinesis, Cdc14p regulated mitotic exit, since synchronous cultures of cdc14{Delta} cells exhibited a severe delay in the destruction of the mitotic cyclin Clb2p. Finally, deletion of CDC14 resulted in decreased invasion of solid agar medium and impaired true hyphal growth.

Key words: Cytokinesis, Cell separation, Mitotic exit, ACE2, HGC1


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Wang, P. P. Raniga, S. Lane, Y. Lu, and H. Liu
Hyphal Chain Formation in Candida albicans: Cdc28-Hgc1 Phosphorylation of Efg1 Represses Cell Separation Genes
Mol. Cell. Biol., August 15, 2009; 29(16): 4406 - 4416.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
N. Hanaoka, Y. Takano, K. Shibuya, H. Fugo, Y. Uehara, and M. Niimi
Identification of the Putative Protein Phosphatase Gene PTC1 as a Virulence-Related Gene Using a Silkworm Model of Candida albicans Infection
Eukaryot. Cell, October 1, 2008; 7(10): 1640 - 1648.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Gonzalez-Novo, J. Correa-Bordes, L. Labrador, M. Sanchez, C. R. Vazquez de Aldana, and J. Jimenez
Sep7 Is Essential to Modify Septin Ring Dynamics and Inhibit Cell Separation during Candida albicans Hyphal Growth
Mol. Biol. Cell, April 1, 2008; 19(4): 1509 - 1518.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
A. Wang, S. Lane, Z. Tian, A. Sharon, I. Hazan, and H. Liu
Temporal and Spatial Control of HGC1 Expression Results in Hgc1 Localization to the Apical Cells of Hyphae in Candida albicans
Eukaryot. Cell, February 1, 2007; 6(2): 253 - 261.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
S. M. Mulhern, M. E. Logue, and G. Butler
Candida albicans Transcription Factor Ace2 Regulates Metabolism and Is Required for Filamentation in Hypoxic Conditions
Eukaryot. Cell, December 1, 2006; 5(12): 2001 - 2013.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2006