spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online 8 May 2007
doi: 10.1242/jcs.005306

Journal of Cell Science 120, 1868-1876 (2007)
Published by The Company of Biologists 2007
This Article
Right arrow Figures Only
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.005306v1
120/11/1868    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Related articles in JCS
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pan, J.
Right arrow Articles by Brody, S. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pan, J.
Right arrow Articles by Brody, S. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

RhoA-mediated apical actin enrichment is required for ciliogenesis and promoted by Foxj1

Jiehong Pan, Yingjian You, Tao Huang and Steven L. Brody*

Pulmonary and Critical Care Medicine, Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA

* Author for correspondence (e-mail: sbrody{at}im.wustl.edu)

Accepted 11 April 2007

Programs that direct cellular differentiation are dependent on the strict temporal expression of regulatory factors that can be provided by Rho GTPases. Ciliogenesis is a complex sequence of events involving the generation and docking of basal bodies at the apical membrane, followed by ciliary axoneme generation. Although a cilia proteome has been assembled, programs that direct ciliated cell differentiation are not well established, particularly in mammalian systems. Using mouse primary culture airway epithelial cells, we identified a critical stage of ciliogenesis requiring the temporal establishment of an apical web-like structure of actin for basal body docking and subsequent axoneme growth. Apical web formation and basal body docking were prevented by interruption of actin remodeling and were dependent on RhoA activation. Additional evidence for this program was provided by analysis of Foxj1-null mice that failed to dock basal bodies and lacked apical actin. Foxj1 expression coincided with actin web formation, activated RhoA and RhoB, and persisted despite RhoA inhibition, suggesting that Foxj1 promoted RhoA during ciliogenesis. Apical ezrin localization was also dependent on Foxj1, actin remodeling, and RhoA, but was not critical for ciliogenesis. Thus, temporal Foxj1 and RhoA activity are essential regulatory events for cytoskeletal remodeling during mammalian ciliogenesis.

Key words: Basal bodies, Cilia, Airway epithelial cells, GTPase, Mouse


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related articles in JCS:

Toxic clues to cadherin processing

JCS 2007 120: 1104. [Full Text]  



This article has been cited by other articles:


Home page
 DevelopmentHome page
B. V. Jacquet, R. Salinas-Mondragon, H. Liang, B. Therit, J. D. Buie, M. Dykstra, K. Campbell, L. E. Ostrowski, S. L. Brody, and H. T. Ghashghaei
FoxJ1-dependent gene expression is required for differentiation of radial glia into ependymal cells and a subset of astrocytes in the postnatal brain
Development, December 1, 2009; 136(23): 4021 - 4031.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. Maouche, M. Polette, T. Jolly, K. Medjber, I. Cloez-Tayarani, J.-P. Changeux, H. Burlet, C. Terryn, C. Coraux, J.-M. Zahm, et al.
{alpha}7 Nicotinic Acetylcholine Receptor Regulates Airway Epithelium Differentiation by Controlling Basal Cell Proliferation
Am. J. Pathol., November 1, 2009; 175(5): 1868 - 1882.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
Y.-C. Hsiao, Z. J. Tong, J. E. Westfall, J. G. Ault, P. S. Page-McCaw, and R. J. Ferland
Ahi1, whose human ortholog is mutated in Joubert syndrome, is required for Rab8a localization, ciliogenesis and vesicle trafficking
Hum. Mol. Genet., October 15, 2009; 18(20): 3926 - 3941.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. Dammermann, H. Pemble, B. J. Mitchell, I. McLeod, J. R. Yates III, C. Kintner, A. B. Desai, and K. Oegema
The hydrolethalus syndrome protein HYLS-1 links core centriole structure to cilia formation
Genes & Dev., September 1, 2009; 23(17): 2046 - 2059.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. R. Dawe, M. Adams, G. Wheway, K. Szymanska, C. V. Logan, A. A. Noegel, K. Gull, and C. A. Johnson
Nesprin-2 interacts with meckelin and mediates ciliogenesis via remodelling of the actin cytoskeleton
J. Cell Sci., August 1, 2009; 122(15): 2716 - 2726.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
Y. Yin, F. Bangs, I. R. Paton, A. Prescott, J. James, M. G. Davey, P. Whitley, G. Genikhovich, U. Technau, D. W. Burt, et al.
The Talpid3 gene (KIAA0586) encodes a centrosomal protein that is essential for primary cilia formation
Development, February 15, 2009; 136(4): 655 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
M Adams, U M Smith, C V Logan, and C A Johnson
Recent advances in the molecular pathology, cell biology and genetics of ciliopathies
J. Med. Genet., May 1, 2008; 45(5): 257 - 267.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007