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First published online 8 May 2007
doi: 10.1242/jcs.03448

Journal of Cell Science 120, 1877-1887 (2007)
Published by The Company of Biologists 2007
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Research Article

Nogo enhances the adhesion of olfactory ensheathing cells and inhibits their migration

Zhida Su*, Li Cao*, Yanling Zhu, Xiujie Liu, Zhihui Huang, Aijun Huang and Cheng He{ddagger}

Department of Neurobiology, Second Military Medical University, Shanghai 200433, China

{ddagger} Author for correspondence (e-mail: chenghe{at}online.sh.cn)

Accepted 28 March 2007

The migration of olfactory ensheathing cells (OECs) is essential for pioneering the olfactory nerve pathway during development and for promoting axonal regeneration when implanted into the injured central nervous system (CNS). In the present study, recombinant Nogo-66 enhanced the adhesion of OECs and inhibited their migration. Using immunocytochemistry and western blot, we showed that the Nogo-66 receptor (NgR) was expressed on OECs. When NgR was released from the cell surface with phosphatidylinositol-specific phospholipase C or neutralized by NgR antibody, the effect of Nogo-66 on OEC adhesion and migration was markedly attenuated. Nogo-66 was found to promote the formation of focal adhesion in OECs and inhibited their membrane protrusion through the activation of RhoA. Furthermore, the co-culture migration assay demonstrated that OEC motility was significantly restricted by Nogo-A expressed on Cos7 cell membranes or oligodendrocytes. Moreover, treatment with anti-NgR antibody facilitated migration of implanted OECs in a spinal cord hemisection injury model. Taken together, we demonstrate, for the first time, that Nogo, a myelin-associated inhibitor of axon regeneration in the CNS, enhances the adhesion and inhibits the migration of OECs via NgR regulation of RhoA.

Key words: Nogo, Olfactory ensheathing cells, Migration, Adhesion, RhoA, Spinal cord injury


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Y. Takei
Phosphorylation of Nogo Receptors Suppresses Nogo Signaling, Allowing Neurite Regeneration
Sci. Signal., March 31, 2009; 2(64): ra14 - ra14.
[Abstract] [Full Text] [PDF]


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