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First published online 24 July 2007
doi: 10.1242/jcs.009506

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A conserved role for kinesin-5 in plant mitosis

¹ Biology Department, University of Massachusetts, Amherst, MA 01003 USA
² Max Planck Institute for Molecular Plant Physiology, Science Park Golm, 14476 Potsdam, Germany
³ Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724, USA
⁴ Carnegie Institution, Department of Plant Biology, Stanford, CA 94305, USA

^* Author for correspondence (e-mail: baskin{at}bio.umass.edu)

Accepted 12 June 2007

The mitotic spindle of vascular plants is assembled and maintained by processes that remain poorly explored at a molecular level. Here, we report that AtKRP125c, one of four kinesin-5 motor proteins in arabidopsis, decorates microtubules throughout the cell cycle and appears to function in both interphase and mitosis. In a temperature-sensitive mutant, interphase cortical microtubules are disorganized at the restrictive temperature and mitotic spindles are massively disrupted, consistent with a defect in the stabilization of anti-parallel microtubules in the spindle midzone, as previously described in kinesin-5 mutants from animals and yeast. AtKRP125c introduced into mammalian epithelial cells by transfection decorates microtubules throughout the cell cycle but is unable to complement the loss of the endogenous kinesin-5 motor (Eg5). These results are among the first reports of any motor with a major role in anastral spindle structure in plants and demonstrate that the conservation of kinesin-5 motor function throughout eukaryotes extends to vascular plants.

Key words: Arabidopsis thaliana, AtKRP125, Cortical microtubules, Eg5, -tubulin, Root morphology

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