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First published online 14 August 2007
doi: 10.1242/jcs.007260

Journal of Cell Science 120, 3147-3154 (2007)
Published by The Company of Biologists 2007
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Research Article

Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays

Min Wu1, Tobias Baumgart2, Stephanie Hammond1, David Holowka1 and Barbara Baird1,*

1 Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA
2 School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA

* Author for correspondence (e-mail: bab13{at}cornell.edu)

Accepted 4 July 2007

Polarized response towards a contact interface is a common theme in intercellular signaling. To visualize spatial regulation of stimulated secretion within a contact region, we exposed IgE-sensitized rat basophilic leukemia (RBL) mast cells to a surface that was patterned on the µm scale with hapten-containing lipid bilayers to activate cell surface IgE-receptor complexes. We find that, within 10 minutes of stimulation, fusion of individual secretory lysosomes is targeted towards the cell-substrate interface, but is spatially segregated from the patterned bilayers and receptor signaling complexes. By contrast, stimulated outward trafficking of recycling endosomes is preferentially targeted towards the patterned bilayers. High spatial resolution of both antigen presentation in these arrays and detection of exocytotic events provides direct evidence for the heterogeneity of polarized responses.

Key words: Differential targeting, IgE receptors, Polarized response, Recycling endosome, Secretory lysosome


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