|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
First published online 16 October 2007
doi: 10.1242/jcs.012138
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Research Article |
1 Dipartimento di Biologia L. Gorini, Università degli Studi di Milano, Via Celoria 26, 20133 Milan, Italy
2 Dipartimento di Scienze Ambientali G. Sarfatti, Università degli Studi di Siena, Via P. A. Mattioli 4, 53100 Siena, Italy
3 CIMAINA, Università degli Studi di Milano, Via Celoria 16, 20133 Milan, Italy
4 CIMA, Università degli Studi di Milano, Via Celoria 16, 20133 Milan, Italy
* Author for correspondence (e-mail: alessandra.moscatelli{at}unimi.it)
Accepted 9 August 2007
In an attempt to dissect endocytosis in Nicotiana tabacum L. pollen tubes, two different probes – positively or negatively charged nanogold – were employed. The destiny of internalized plasma membrane domains, carrying negatively or positively charged residues, was followed at the ultrastructural level and revealed distinct endocytic pathways. Time-course experiments and electron microscopy showed internalization of subapical plasma-membrane domains that were mainly recycled to the secretory pathway through the Golgi apparatus and a second mainly degradative pathway involving plasma membrane retrieval at the tip. In vivo time-lapse experiments using FM4-64 combined with quantitative analysis confirmed the existence of distinct internalization regions. Ikarugamycin, an inhibitor of clathrin-dependent endocytosis, allowed us to further dissect the endocytic process: electron microscopy and time-lapse studies suggested that clathrin-dependent endocytosis occurs in the tip and subapical regions, because recycling of positively charged nanogold to the Golgi bodies and the consignment of negatively charged nanogold to vacuoles were affected. However, intact positively charged-nanogold transport to vacuoles supports the idea that an endocytic pathway that does not require clathrin is also present in pollen tubes.
Key words: Nicotiana tabacum (L.), Pollen tube, Endocytosis
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati 
Twitter What's this?
Related articles in JCS:
This article has been cited by other articles:
|
|
 |
|
  S. Wolf, G. Mouille, and J. Pelloux Homogalacturonan Methyl-Esterification and Plant Development Mol Plant, September 1, 2009; 2(5): 851 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cai and M. Cresti Organelle motility in the pollen tube: a tale of 20 years J. Exp. Bot., February 1, 2009; 60(2): 495 - 508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Lee, S. Kim, and B. McClure A Pollen Protein, NaPCCP, That Binds Pistil Arabinogalactan Proteins Also Binds Phosphatidylinositol 3-Phosphate and Associates with the Pollen Tube Endomembrane System Plant Physiology, February 1, 2009; 149(2): 791 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ischebeck, I. Stenzel, and I. Heilmann Type B Phosphatidylinositol-4-Phosphate 5-Kinases Mediate Arabidopsis and Nicotiana tabacum Pollen Tube Growth by Regulating Apical Pectin Secretion PLANT CELL, December 1, 2008; 20(12): 3312 - 3330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sousa, B. Kost, and R. Malho Arabidopsis Phosphatidylinositol-4-Monophosphate 5-Kinase 4 Regulates Pollen Tube Growth and Polarity by Modulating Membrane Recycling PLANT CELL, November 1, 2008; 20(11): 3050 - 3064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. B. Lee, K. N. Swatek, and B. McClure Pollen Proteins Bind to the C-terminal Domain of Nicotiana alata Pistil Arabinogalactan Proteins J. Biol. Chem., October 3, 2008; 283(40): 26965 - 26973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Onelli, C. Prescianotto-Baschong, M. Caccianiga, and A. Moscatelli Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold J. Exp. Bot., August 1, 2008; 59(11): 3051 - 3068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Yalovsky, D. Bloch, N. Sorek, and B. Kost Regulation of Membrane Trafficking, Cytoskeleton Dynamics, and Cell Polarity by ROP/RAC GTPases Plant Physiology, August 1, 2008; 147(4): 1527 - 1543. [Full Text] [PDF]
|
|
|
|
|
|
J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, and A. Geitmann Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching Plant Physiology, August 1, 2008; 147(4): 1646 - 1658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zonia and T. Munnik Vesicle trafficking dynamics and visualization of zones of exocytosis and endocytosis in tobacco pollen tubes J. Exp. Bot., March 1, 2008; 59(4): 861 - 873. [Abstract] [Full Text] [PDF]
|
|
