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First published online 10 June 2008
doi: 10.1242/jcs.019745

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Interaction of Mint3 with Furin regulates the localization of Furin in the trans-Golgi network

Jinbo Han^1,2,*, Yiguo Wang^1,*, Suming Wang^3,* and Chengwu Chi^1,2,

¹ Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, People's Republic of China
² Institute of Protein Research, Tongji University, Shanghai 200092, People's Republic of China
³ School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, People's Republic of China

Author for correspondence (e-mail: zwqi{at}sibs.ac.cn)

Accepted 9 April 2008

Furin is a proprotein convertase that cycles between the plasma membrane, endosomes and the trans-Golgi network (TGN), maintaining a predominant distribution in the latter. Mint3, a member of the Mint protein family, is involved in the signaling and trafficking of membrane proteins. Until now, little has been known about the roles of Mint3 in the localization or trafficking of Furin. Here, using co-immunoprecipitation and immunofluorescence assays, we show that Mint3 interacts with Furin in the Golgi compartment of HeLa cells. Knockdown of endogenous Mint3 expression by RNA interference disrupts the TGN-specific localization of Furin and increases its distribution in endosomes. We further demonstrate that the phosphotyrosine-binding (PTB) domain of Mint3 is essential for the binding of Furin and that this binding affects the TGN-specific localization of Furin. Moreover, mutation studies of Furin indicate that Mint3 regulates Furin distribution mainly through interaction with the acidic peptide signal of Furin. Collectively, these data suggest that the interaction between the PTB domain of Mint3 and the acidic peptide signal of Furin regulates the specific localization of Furin in the TGN.

Key words: Acidic peptide signal, Furin, Mint3, Phosphotyrosine-binding domain, Trans-Golgi network

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