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First published online 26 February 2008
doi: 10.1242/jcs.021584

Journal of Cell Science 121, 843-853 (2008)
Published by The Company of Biologists 2008
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Research Article

Chemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network

Sandra Dischinger1,2, Andrea Krapp1,2, Linfeng Xie3, James R. Paulson3 and Viesturs Simanis1,2,*

1 Cell cycle control laboratory, ISREC, 1066 Epalinges, Switzerland
2 Faculty of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Switzerland
3 Department of Chemistry, University of Wisconsin-Oshkosh, 800 Algoma Boulevard, Oshkosh, WI 54901, USA

* Author for correspondence (e-mail: viesturs.simanis{at}epfl.ch)

Accepted 24 December 2007

The protein kinase Cdc2p is the master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. It is required both for entry into mitosis and for onset of DNA replication. Cdc2p must be inactivated to permit exit from mitosis, licensing of replication origins and cytokinesis. To study the role of Cdc2p in greater detail, we generated a cdc2 allele that is sensitive to an inhibitory ATP analogue. We show that the inhibitor-induced cell cycle arrest is reversible and examine the effect of inhibiting Cdc2p on the regulation of the septation initiation network (SIN), which controls the initiation of cytokinesis in S. pombe. We found that specific inactivation of Cdc2p in a mitotically arrested cell promotes the asymmetrical recruitment of SIN proteins to the spindle poles and the recruitment of the most downstream SIN components and β-(1,3) glucan synthase to the contractile ring. Thus, we conclude that inactivation of Cdc2p is sufficient to activate the SIN and promote cytokinesis.

Key words: Chemical genetics, Cell cycle, cdc2, Cytokinesis, Yeast


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