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First published online 14 April 2008
doi: 10.1242/jcs.027466

Journal of Cell Science 121, 1444-1454 (2008)
Published by The Company of Biologists 2008
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Research Article

ABBA regulates plasma-membrane and actin dynamics to promote radial glia extension

Juha Saarikangas1, Janne Hakanen2,3, Pieta K. Mattila1, Martin Grumet4, Marjo Salminen2,3 and Pekka Lappalainen1,5,*

1 Program in Cellular Biotechnology, Institute of Biotechnology, PO Box 56, 00014 University of Helsinki, Finland
2 Program in Developmental Biology, Institute of Biotechnology, PO Box 56, 00014 University of Helsinki, Finland
3 Division of Biochemistry, Department of Basic Veterinary Sciences, PO Box 66, 00014 University of Helsinki, Finland
4 W. M. Keck Center for Collaborative Neuroscience, Rutgers, State University of New Jersey, NJ, USA
5 Neuroscience Center, PO Box 56, 00014 University of Helsinki, Finland

* Author for correspondence (e-mail: pekka.lappalainen{at}helsinki.fi)

Accepted 5 February 2008

Radial glia play key roles in neuronal migration, axon guidance, and neurogenesis during development of the central nervous system. However, the molecular mechanisms regulating growth and morphology of these extended cells are unknown. We show that ABBA, a novel member of the IRSp53-MIM protein family, is enriched in different types of radial glia. ABBA binds ATP-actin monomers with high affinity and deforms PtdIns(4,5)P2-rich membranes in vitro through its WH2 and IM domains, respectively. In radial-glia-like C6-R cells, ABBA localises to the interface between the actin cytoskeleton and plasma membrane, and its depletion by RNAi led to defects in lamellipodial dynamics and process extension. Together, this study identifies ABBA as a novel regulator of actin and plasma membrane dynamics in radial glial cells, and provides evidence that membrane binding and deformation activity is critical for the cellular functions of IRSp53-MIM-ABBA family proteins.

Key words: ABBA, IMD, Actin, Cytoskeleton, Membrane deformation, Radial glia


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