spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 20 Nov 2002
doi: 10.1242/jcs.00228

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00228v1
116/2/285    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chabregas, S. M.
Right arrow Articles by Silva-Filho, M. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chabregas, S. M.
Right arrow Articles by Silva-Filho, M. C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Differential usage of two in-frame translational start codons regulates subcellular localization of Arabidopsis thaliana THI1


Sabrina M. Chabregas, Douglas D. Luche, Marie-Anne Van Sluys, Carlos F.M. Menck, and Marcio C. Silva-Filho*
* Author for correspondence (e-mail: mdcsilva{at}esalq.usp.br)

Arabidopsis thaliana THI1 is encoded by a single nuclear gene and directed simultaneously to mitochondria and chloroplasts from a single major transcript. In vitro transcription/translation experiments revealed the presence of two translational products by the differential usage of two in-frame translational start codons. The coupling site-specific mutations on the THI1 encoding sequence with green fluorescent protein (GFP) gene fusions showed that translation initiation at the first AUG directs translocation of THI1 to chloroplasts. However, when translation starts from the second AUG, THI1 is addressed to mitochondria. Analysis of the translation efficiency of thi1 mRNA revealed that the best context for translation initiation is to use the first AUG. In addition, a suboptimal context in the vicinity of the second AUG initiation codon, next to a stable stem-and-loop structure that is likely to slow translation, has been noted. The fact that translation preferentially occurs in the first AUG of this protein suggests a high requirement for TH1 in chloroplasts. Although the frequency of upstream AUG translation is higher, according to the first AUG rule, initiation at the second AUG deviates significantly from Kozak's consensus. It suggests leaky ribosomal scanning, reinitiation or the internal entry of ribosomes to assure mitochondrial protein import.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. Rahmani, M. Hummel, J. Schuurmans, A. Wiese-Klinkenberg, S. Smeekens, and J. Hanson
Sucrose Control of Translation Mediated by an Upstream Open Reading Frame-Encoded Peptide
Plant Physiology, July 1, 2009; 150(3): 1356 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. H. Millar, C. Carrie, B. Pogson, and J. Whelan
Exploring the Function-Location Nexus: Using Multiple Lines of Evidence in Defining the Subcellular Location of Plant Proteins
PLANT CELL, June 1, 2009; 21(6): 1625 - 1631.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Huang, N. L. Taylor, R. Narsai, H. Eubel, J. Whelan, and A. H. Millar
Experimental Analysis of the Rice Mitochondrial Proteome, Its Biogenesis, and Heterogeneity
Plant Physiology, February 1, 2009; 149(2): 719 - 734.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. A. Mudd, S. Sullivan, M. F. Gisby, A. Mironov, C. S. Kwon, W.-I. Chung, and A. Day
A 125 kDa RNase E/G-like protein is present in plastids and is essential for chloroplast development and autotrophic growth in Arabidopsis
J. Exp. Bot., July 1, 2008; 59(10): 2597 - 2610.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Schmidt von Braun, A. Sabetti, P. J. Hanic-Joyce, J. Gu, E. Schleiff, and P. B. M. Joyce
Dual targeting of the tRNA nucleotidyltransferase in plants: not just the signal
J. Exp. Bot., December 1, 2007; 58(15-16): 4083 - 4093.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. E. Lunn
Compartmentation in plant metabolism
J. Exp. Bot., January 1, 2007; 58(1): 35 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J.-B. Peltier, Y. Cai, Q. Sun, V. Zabrouskov, L. Giacomelli, A. Rudella, A. J. Ytterberg, H. Rutschow, and K. J. van Wijk
The Oligomeric Stromal Proteome of Arabidopsis thaliana Chloroplasts
Mol. Cell. Proteomics, January 1, 2006; 5(1): 114 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. T. Ribeiro, L. P. Farias, J. D. de Almeida, P. M. Kashiwabara, A. F. C. Ribeiro, M. C. Silva-Filho, C. F. M. Menck, and M.-A. Van Sluys
Functional characterization of the thi1 promoter region from Arabidopsis thaliana
J. Exp. Bot., July 1, 2005; 56(417): 1797 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. D. Hare, S. G. Moller, L.-F. Huang, and N.-H. Chua
LAF3, a Novel Factor Required for Normal Phytochrome A Signaling
Plant Physiology, December 1, 2003; 133(4): 1592 - 1604.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
O. Chew, J. Whelan, and A. H. Millar
Molecular Definition of the Ascorbate-Glutathione Cycle in Arabidopsis Mitochondria Reveals Dual Targeting of Antioxidant Defenses in Plants
J. Biol. Chem., November 21, 2003; 278(47): 46869 - 46877.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
F. S. Papini-Terzi, R. d. S. Galhardo, L. P. Farias, C. F. M. Menck, and M.-A. Van Sluys
Point Mutation is Responsible for Arabidopsis tz-201 Mutant Phenotype Affecting Thiamin Biosynthesis
Plant Cell Physiol., August 15, 2003; 44(8): 856 - 860.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2002