spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 27 Nov 2002
doi: 10.1242/jcs.00234

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00234v1
116/2/345    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Michaelson, J. S.
Right arrow Articles by Leder, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Michaelson, J. S.
Right arrow Articles by Leder, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

RNAi reveals anti-apoptotic and transcriptionally repressive activities of DAXX


Jennifer S. Michaelson and Philip Leder*
* Author for correspondence (e-mail: leder{at}rascal.med.harvard.edu)

The function of DAXX, a highly conserved mammalian gene, has remained controversial; this is due, in part, to its identification in a variety of yeast two-hybrid screens. Targeted deletion in the mouse revealed that DAXX is essential for embryonic development. Furthermore, the increased levels of apoptosis observed in Daxx-knockout embryos and embryonic stem cell lines suggested that DAXX functions in an anti-apoptotic capacity. In contrast, overexpression studies showed that DAXX may promote apoptosis. Additional studies showed that, when overexpressed, DAXX could function as a transcriptional repressor. To clarify these matters, we have used RNAi to deplete endogenous DAXX and thereby assess DAXX function in cell lines previously tested in overexpression studies. Increased apoptosis was observed in DAXX-depleted cells, showing DAXX to be anti-apoptotic. The apoptosis induced by the absence of DAXX was rescued by Bcl-2 overexpression. In addition, transcriptional de-repression was observed in RNAi-treated cells, indicating the ability of endogenous DAXX to repress gene expression and allowing for the identification of novel targets of DAXX repression, including nuclear factor {kappa}B (NF-{kappa}B)- and E2F1- regulated targets. Thus, depletion of DAXX by RNAi has verified the crucial role of endogenous DAXX as an anti-apoptotic regulator, and has allowed the identification of probable physiological targets of DAXX transcriptional repression.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
T. Kitamura, Y. Fukuyo, M. Inoue, N. T. Horikoshi, M. Shindoh, B. E. Rogers, A. Usheva, and N. Horikoshi
Mutant p53 Disrupts the Stress MAPK Activation Circuit Induced by ASK1-Dependent Stabilization of Daxx
Cancer Res., October 1, 2009; 69(19): 7681 - 7688.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. J. Ross, M. A. Kennedy, and R. J. Parks
Host Cell Detection of Noncoding Stuffer DNA Contained in Helper-Dependent Adenovirus Vectors Leads to Epigenetic Repression of Transgene Expression
J. Virol., September 1, 2009; 83(17): 8409 - 8417.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Lukashchuk, S. McFarlane, R. D. Everett, and C. M. Preston
Human Cytomegalovirus Protein pp71 Displaces the Chromatin-Associated Factor ATRX from Nuclear Domain 10 at Early Stages of Infection
J. Virol., December 15, 2008; 82(24): 12543 - 12554.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. J. Ullman and P. Hearing
Cellular Proteins PML and Daxx Mediate an Innate Antiviral Defense Antagonized by the Adenovirus E4 ORF3 Protein
J. Virol., August 1, 2008; 82(15): 7325 - 7335.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. D. Everett, C. Parada, P. Gripon, H. Sirma, and A. Orr
Replication of ICP0-Null Mutant Herpes Simplex Virus Type 1 Is Restricted by both PML and Sp100
J. Virol., March 15, 2008; 82(6): 2661 - 2672.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-S. Jung, H.-Y. Kim, J. Kim, M.-G. Lee, J. Pouyssegur, and E. Kim
Physical Interactions and Functional Coupling between Daxx and Sodium Hydrogen Exchanger 1 in Ischemic Cell Death
J. Biol. Chem., January 11, 2008; 283(2): 1018 - 1025.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
I. J. Groves and J. H. Sinclair
Knockdown of hDaxx in normally non-permissive undifferentiated cells does not permit human cytomegalovirus immediate-early gene expression
J. Gen. Virol., November 1, 2007; 88(11): 2935 - 2940.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. T. Saffert and R. F. Kalejta
Human Cytomegalovirus Gene Expression Is Silenced by Daxx-Mediated Intrinsic Immune Defense in Model Latent Infections Established In Vitro
J. Virol., September 1, 2007; 81(17): 9109 - 9120.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
A. Chakraborty, H. Brooks, P. Zhang, W. Smith, M. R. McReynolds, J. B. Hoying, R. Bick, L. Truong, B. Poindexter, H. Lan, et al.
Stanniocalcin-1 regulates endothelial gene expression and modulates transendothelial migration of leukocytes
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F895 - F904.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Q. Li, X. Wang, X. Wu, Y. Rui, W. Liu, J. Wang, X. Wang, Y.-C. Liou, Z. Ye, and S.-C. Lin
Daxx Cooperates with the Axin/HIPK2/p53 Complex to Induce Cell Death
Cancer Res., January 1, 2007; 67(1): 66 - 74.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. L. Woodhall, I. J. Groves, M. B. Reeves, G. Wilkinson, and J. H. Sinclair
Human Daxx-mediated Repression of Human Cytomegalovirus Gene Expression Correlates with a Repressive Chromatin Structure around the Major Immediate Early Promoter
J. Biol. Chem., December 8, 2006; 281(49): 37652 - 37660.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. Croxton, L. A. Puto, I. de Belle, M. Thomas, S. Torii, F. Hanaii, M. Cuddy, and J. C. Reed
Daxx Represses Expression of a Subset of Antiapoptotic Genes Regulated by Nuclear Factor-{kappa}B.
Cancer Res., September 15, 2006; 66(18): 9026 - 9035.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. R. Cantrell and W. A. Bresnahan
Human Cytomegalovirus (HCMV) UL82 Gene Product (pp71) Relieves hDaxx-Mediated Repression of HCMV Replication.
J. Virol., June 1, 2006; 80(12): 6188 - 6191.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
C. M. Preston and M. J. Nicholl
Role of the cellular protein hDaxx in human cytomegalovirus immediate-early gene expression.
J. Gen. Virol., May 1, 2006; 87(Pt 5): 1113 - 1121.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. T. Saffert and R. F. Kalejta
Inactivating a Cellular Intrinsic Immune Defense Mediated by Daxx Is the Mechanism through Which the Human Cytomegalovirus pp71 Protein Stimulates Viral Immediate-Early Gene Expression.
J. Virol., April 1, 2006; 80(8): 3863 - 3871.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. R. Cantrell and W. A. Bresnahan
Interaction between the Human Cytomegalovirus UL82 Gene Product (pp71) and hDaxx Regulates Immediate-Early Gene Expression and Viral Replication
J. Virol., June 15, 2005; 79(12): 7792 - 7802.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Tang, M. Kmet, L. Corral, S. Vartanian, A. Tobler, and J. Papkoff
Testisin, a Glycosyl-Phosphatidylinositol-Linked Serine Protease, Promotes Malignant Transformation In vitro and In vivo
Cancer Res., February 1, 2005; 65(3): 868 - 878.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Crowder, O. Dahle, R. E. Davis, O. S. Gabrielsen, and S. Rudikoff
PML mediates IFN-{alpha}-induced apoptosis in myeloma by regulating TRAIL induction
Blood, February 1, 2005; 105(3): 1280 - 1287.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Y. Zhao, J. Liu, G. S. Sidhu, Y. Niu, Y. Liu, R. Wang, and D. Liao
Negative Regulation of p53 Functions by Daxx and the Involvement of MDM2
J. Biol. Chem., November 26, 2004; 279(48): 50566 - 50579.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Gostissa, M. Morelli, F. Mantovani, E. Guida, S. Piazza, L. Collavin, C. Brancolini, C. Schneider, and G. Del Sal
The Transcriptional Repressor hDaxx Potentiates p53-dependent Apoptosis
J. Biol. Chem., November 12, 2004; 279(46): 48013 - 48023.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. M. Ishov, O. V. Vladimirova, and G. G. Maul
Heterochromatin and ND10 are cell-cycle regulated and phosphorylation-dependent alternate nuclear sites of the transcription repressor Daxx and SWI/SNF protein ATRX
J. Cell Sci., August 1, 2004; 117(17): 3807 - 3820.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Boellmann, T. Guettouche, Y. Guo, M. Fenna, L. Mnayer, and R. Voellmy
DAXX interacts with heat shock factor 1 during stress activation and enhances its transcriptional activity
PNAS, March 23, 2004; 101(12): 4100 - 4105.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
D. Obradovic, M. Tirard, Zs. Nemethy, O. Hirsch, H. Gronemeyer, and O. F. X. Almeida
DAXX, FLASH, and FAF-1 Modulate Mineralocorticoid and Glucocorticoid Receptor-Mediated Transcription in Hippocampal Cells--Toward a Basis for the Opposite Actions Elicited by Two Nuclear Receptors?
Mol. Pharmacol., March 1, 2004; 65(3): 761 - 769.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
R. Muromoto, K. Sugiyama, A. Takachi, S. Imoto, N. Sato, T. Yamamoto, K. Oritani, K. Shimoda, and T. Matsuda
Physical and Functional Interactions between Daxx and DNA Methyltransferase 1-Associated Protein, DMAP1
J. Immunol., March 1, 2004; 172(5): 2985 - 2993.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L.-Y. Chen and J. D. Chen
Daxx Silencing Sensitizes Cells to Multiple Apoptotic Pathways
Mol. Cell. Biol., October 15, 2003; 23(20): 7108 - 7121.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E.-J. Kim, J.-S. Park, and S.-J. Um
Identification of Daxx interacting with p73, one of the p53 family, and its regulation of p53 activity by competitive interaction with PML
Nucleic Acids Res., September 15, 2003; 31(18): 5356 - 5367.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2002