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JCS ePress online publication date 11 Mar 2003
doi: 10.1242/jcs.00311

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116/9/1667    most recent
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Research Article

Nucleolar association of pEg7 and XCAP-E, two members of Xenopus laevis condensin complex in interphase cells


Rustem Uzbekov, Elmira Timirbulatova, Erwan Watrin, Fabien Cubizolles, David Ogereau, Pavel Gulak, Vincent Legagneux, Vladimir Ju. Polyakov, Katherine Le Guellec, and Igor Kireev*
* Author for correspondence (e-mail: ikireev{at}life.uiuc.edu)

Cell cycle dynamics and localization of condensins - multiprotein complexes involved in late stages of mitotic chromosome condensation - were studied in Xenopus laevis XL2 cell line. Western blot analysis of synchronized cells showed that the ratio of levels of both pEg7 and XCAP-E to {beta}-tubulin levels remains almost constant from G1 to M phase. pEg7 and XCAP-E were localized to the mitotic chromosomes and were detected in interphase nuclei. Immunostaining for condensins and nucleolar proteins UBF, fibrillarin and B23 revealed that both XCAP-E and pEg7 are localized in the granular component of the nucleolus. Nucleolar labeling of both proteins is preserved in segregated nucleoli after 6 hours of incubation with actinomycin D (5 mg/ml), but the size of the labeled zone was significantly smaller. The data suggest a novel interphase function of condensin subunits in spatial organization of the nucleolus and/or ribosome biogenesis.
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