spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 11 Mar 2003
doi: 10.1242/jcs.00371

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00371v1
116/9/1805    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Desterro, J. M. P.
Right arrow Articles by Carmo-Fonseca, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Desterro, J. M. P.
Right arrow Articles by Carmo-Fonseca, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Dynamic association of RNA-editing enzymes with the nucleolus


Joana M. P. Desterro, Liam P. Keegan, Miguel Lafarga, Maria Teresa Berciano, Mary O'Connell, and Maria Carmo-Fonseca*
* Author for correspondence (e-mail: carmo.fonseca{at}fm.ul.pt)

ADAR1 and ADAR2 are editing enzymes that deaminate adenosine to inosine in long double stranded RNA duplexes and specific pre-mRNA transcripts. Here, we show that full-length and N-terminally truncated forms of ADAR1 are simultaneously expressed in HeLa and COS7 cells owing to the usage of alternative starting methionines. Because the N-terminus of ADAR1 contains a nuclear export signal, the full-length protein localizes predominantly in the cytoplasm, whereas the N-terminally truncated forms are exclusively nuclear and accumulate in the nucleolus. ADAR2, which lacks a region homologous to the N-terminal domain of ADAR1, localizes exclusively to the nucleus and similarly accumulates in the nucleolus. Within the nucleolus, ADAR1 and ADAR2 co-localize in a novel compartment. Photobleaching experiments demonstrate that, in live cells, ADAR1 and ADAR2 are in constant flux in and out of the nucleolus. When cells express the editing-competent glutamate receptor GluR-B RNA, endogenous ADAR1 and ADAR2 de-localize from the nucleolus and accumulate at sites where the substrate transcripts accumulate. This suggests that ADAR1 and ADAR2 are constantly moving through the nucleolus and might be recruited onto specific editing substrates present elsewhere in the cell.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
S. Maas and W. M. Gommans
Identification of a selective nuclear import signal in adenosine deaminases acting on RNA
Nucleic Acids Res., September 1, 2009; 37(17): 5822 - 5829.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
H. A. Hundley, A. A. Krauchuk, and B. L. Bass
C. elegans and H. sapiens mRNAs with edited 3' UTRs are present on polysomes
RNA, October 1, 2008; 14(10): 2050 - 2060.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
H. Ohta, M. Fujiwara, Y. Ohshima, and T. Ishihara
ADBP-1 Regulates an ADAR RNA-Editing Enzyme to Antagonize RNA-Interference-Mediated Gene Silencing in Caenorhabditis elegans
Genetics, October 1, 2008; 180(2): 785 - 796.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Cenci, R. Barzotti, F. Galeano, S. Corbelli, R. Rota, L. Massimi, C. Di Rocco, M. A. O'Connell, and A. Gallo
Down-regulation of RNA Editing in Pediatric Astrocytomas: ADAR2 EDITING ACTIVITY INHIBITS CELL MIGRATION AND PROLIFERATION
J. Biol. Chem., March 14, 2008; 283(11): 7251 - 7260.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
S. Lykke-Andersen, S. Pinol-Roma, and J. Kjems
Alternative splicing of the ADAR1 transcript in a region that functions either as a 5'-UTR or an ORF
RNA, October 1, 2007; 13(10): 1732 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
J. Ohlson, J. S. Pedersen, D. Haussler, and M. Ohman
Editing modifies the GABAA receptor subunit {alpha}3
RNA, May 1, 2007; 13(5): 698 - 703.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. C. Ritland Politz, F. Zhang, and T. Pederson
MicroRNA-206 colocalizes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myogenic cells
PNAS, December 12, 2006; 103(50): 18957 - 18962.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
H. Poulsen, R. Jorgensen, A. Heding, F. C. Nielsen, B. Bonven, and J. Egebjerg
Dimerization of ADAR2 is mediated by the double-stranded RNA binding domain
RNA, July 1, 2006; 12(7): 1350 - 1360.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
M. Xu, K. S. Wells, and R. B. Emeson
Substrate-dependent Contribution of Double-stranded RNA-binding Motifs to ADAR2 Function
Mol. Biol. Cell, July 1, 2006; 17(7): 3211 - 3220.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Chilibeck, T. Wu, C. Liang, M. J. Schellenberg, E. M. Gesner, J. M. Lynch, and A. M. MacMillan
FRET Analysis of in Vivo Dimerization by RNA-editing Enzymes
J. Biol. Chem., June 16, 2006; 281(24): 16530 - 16535.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V. Torrano, J. Navascues, F. Docquier, R. Zhang, L. J. Burke, I. Chernukhin, D. Farrar, J. Leon, M. T. Berciano, R. Renkawitz, et al.
Targeting of CTCF to the nucleolus inhibits nucleolar transcription through a poly(ADP-ribosyl)ation-dependent mechanism
J. Cell Sci., May 1, 2006; 119(9): 1746 - 1759.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Feng, C. L. Sansam, M. Singh, and R. B. Emeson
Altered RNA Editing in Mice Lacking ADAR2 Autoregulation
Mol. Cell. Biol., January 15, 2006; 26(2): 480 - 488.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Yang, Q. Wang, K. L. Howell, J. T. Lee, D.-S. C. Cho, J. M. Murray, and K. Nishikura
ADAR1 RNA Deaminase Limits Short Interfering RNA Efficacy in Mammalian Cells
J. Biol. Chem., February 4, 2005; 280(5): 3946 - 3953.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Nie, Q. Zhao, Y. Su, and J.-H. Yang
Subcellular Distribution of ADAR1 Isoforms Is Synergistically Determined by Three Nuclear Discrimination Signals and a Regulatory Motif
J. Biol. Chem., March 26, 2004; 279(13): 13249 - 13255.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. L. Sansam, K. S. Wells, and R. B. Emeson
Modulation of RNA editing by functional nucleolar sequestration of ADAR2
PNAS, November 25, 2003; 100(24): 14018 - 14023.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003