|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search | |||||
The fully linked HTML version of this article has now been published.
Rit, by sequence homology, is a member of the Ras subfamily of small guanine triphosphatases (GTPases). In PC6 cells, Rit signals through pathways both common to and different from those activated by Ras to promote cell survival and neurite outgrowth. However, the specific morphological changes induced by Rit in human cells are not known. Here, we show in a human neuronal model that Rit increases neurite outgrowth and branching through MEK-dependent and MEK-independent signaling mechanisms, respectively. Adenoviral expression of wild-type or constitutively active Rit increased neurite initiation, elongation and branching on endogenous matrix or a purified laminin-1 substratum of SH-SY5Y cells as assessed using image analysis. This outgrowth was morphologically distinct from that promoted by constitutively active Ras or Raf (evidenced by increased branching and elongation). Constitutively active Rit increased phosphorylation of ERK 1/2, but not Akt, and the MEK inhibitor PD 098059 blocked constitutively active Rit-induced neurite initiation but not elongation or branching. These results suggest that Rit plays a key role in human neuronal development and regeneration through activating both known and as yet undefined signaling pathways.
This article has been cited by other articles:
JCS ePress
online publication date 26 Mar 2003
doi: 10.1242/jcs.00401
This Article 
Full Text (PDF)
All Versions of this Article:
jcs.00401v1
116/10/1925
most recent
Alert me when this article is cited
Alert me if a correction is posted
Services
Email this article to a friend
Similar articles in this journal
Similar articles in PubMed
Alert me to new issues of the journal
Download to citation manager
Citing Articles
Citing Articles via HighWire
Citing Articles via Google Scholar
Google Scholar
Articles by Hynds, D. L.
Articles by Snow, D. M.
Search for Related Content
PubMed
PubMed Citation
Articles by Hynds, D. L.
Articles by Snow, D. M.
Social Bookmarking
What's this?
Research Article
Rit promotes MEK-independent neurite branching in human neuroblastoma cells
* Author for correspondence (e-mail: dlhynd0{at}uky.edu)
CiteULike Complore Connotea Del.icio.us Digg Reddit Technorati Twitter What's this?
S. Kaech, G. Banker, and P. Stork
Putting on the RITz
Sci. Signal.,
December 11, 2007;
2007(416):
pe71 - pe71.
[Abstract]
[Full Text]
[PDF]
P. J. Lein, X. Guo, G.-X. Shi, M. Moholt-Siebert, D. Bruun, and D. A. Andres
The Novel GTPase Rit Differentially Regulates Axonal and Dendritic Growth
J. Neurosci.,
April 25, 2007;
27(17):
4725 - 4736.
[Abstract]
[Full Text]
[PDF]
G.-X. Shi, H. Rehmann, and D. A. Andres
A Novel Cyclic AMP-Dependent Epac-Rit Signaling Pathway Contributes to PACAP38-Mediated Neuronal Differentiation
Mol. Cell. Biol.,
December 1, 2006;
26(23):
9136 - 9147.
[Abstract]
[Full Text]
[PDF]
G.-X. Shi, J. Han, and D. A. Andres
Rin GTPase Couples Nerve Growth Factor Signaling to p38 and b-Raf/ERK Pathways to Promote Neuronal Differentiation
J. Biol. Chem.,
November 11, 2005;
280(45):
37599 - 37609.
[Abstract]
[Full Text]
[PDF]
G.-X. Shi and D. A. Andres
Rit Contributes to Nerve Growth Factor-Induced Neuronal Differentiation via Activation of B-Raf-Extracellular Signal-Regulated Kinase and p38 Mitogen-Activated Protein Kinase Cascades
Mol. Cell. Biol.,
January 15, 2005;
25(2):
830 - 846.
[Abstract]
[Full Text]
[PDF]
J. Colicelli
Human RAS Superfamily Proteins and Related GTPases
Sci. Signal.,
September 14, 2004;
2004(250):
re13 - re13.
[Abstract]
[Full Text]
[PDF]
© The Company of Biologists Ltd 2003