spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 30 Apr 2003
doi: 10.1242/jcs.00434

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00434v1
116/12/2399    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peterson, E. A.
Right arrow Articles by Pryzdial, E. L.G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peterson, E. A.
Right arrow Articles by Pryzdial, E. L.G.

Research Article

Thrombin induces endothelial cell-surface exposure of the plasminogen receptor annexin 2


Erica A. Peterson, Michael R. Sutherland, Michael E. Nesheim, and Edward L.G. Pryzdial*
* Author for correspondence (e-mail: ed.pryzdial{at}bloodservices.ca)

Cell-surface annexin 2 (A2) and its ligand p11 have been implicated in fibrinolysis because of their ability to accelerate tissue plasminogen activator (tPA)-mediated activation of plasminogen to plasmin. Because thrombin is a potent cell modulator obligately produced at the site of clot formation, we hypothesized that the amount of cell-surface A2 and p11 might be altered by thrombin with consequent effects on plasmin generation. In support of this hypothesis, immunofluorescence microscopy and hydrophilic biotinylation experiments showed that both A2 and p11 were significantly increased on the surface of human umbilical vein endothelial cells (HUVECs) treated with thrombin (0.8-8 nM) for 5 minutes followed by 1 hour at 37°C. Intracellular immunofluorescence microscopy and immunoblot analyses of whole cell extracts revealed increased p11 but unchanged A2 in response to thrombin, suggesting that transbilayer trafficking of A2 might be controlled by p11. The thrombin receptor-activating peptide (TRAP) similarly affected cells, demonstrating that cell signaling at least involved the type-1 protease activated receptor (PAR-1). An effect on the fibrinolysis pathway after treatment of HUVECs with thrombin was shown by increased fluorescein-labeled plasminogen binding to cells, which was inhibited by an antibody specific for p11. This was confirmed by observing that thrombin pretreatment of HUVECs increased biotin-modified plasminogen binding. Utilizing a chromogenic assay, pretreatment of HUVECs by thrombin further enhanced activation of the Glu and Lys forms of plasminogen by tPA. These data suggest a novel mechanism that links the coagulation and fibrinolysis pathways by thrombin-mediated feedback.


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K.-L. He, A. B. Deora, H. Xiong, Q. Ling, B. B. Weksler, R. Niesvizky, and K. A. Hajjar
Endothelial Cell Annexin A2 Regulates Polyubiquitination and Degradation of Its Binding Partner S100A10/p11
J. Biol. Chem., July 11, 2008; 283(28): 19192 - 19200.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
B. J. Patchell, K. R. Wojcik, T.-L. Yang, S. R. White, and D. R. Dorscheid
Glycosylation and annexin II cell surface translocation mediate airway epithelial wound repair
Am J Physiol Lung Cell Mol Physiol, August 1, 2007; 293(2): L354 - L363.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. C. Derry, M. R. Sutherland, C. M. Restall, D. M. Waisman, and E. L. G. Pryzdial
Annexin 2-mediated enhancement of cytomegalovirus infection opposes inhibition by annexin 1 or annexin 5
J. Gen. Virol., January 1, 2007; 88(1): 19 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. S. Mambula and S. K. Calderwood
Heat Shock Protein 70 Is Secreted from Tumor Cells by a Nonclassical Pathway Involving Lysosomal Endosomes
J. Immunol., December 1, 2006; 177(11): 7849 - 7857.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Watanabe, S. Kondo, K. Mizuno, W. Yano, H. Nakao, Y. Hattori, K. Kimura, and T. Nishida
Promotion of Corneal Epithelial Wound Healing In Vitro and In Vivo by Annexin A5
Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1862 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Laumonnier, T. Syrovets, L. Burysek, and T. Simmet
Identification of the annexin A2 heterotetramer as a receptor for the plasmin-induced signaling in human peripheral monocytes
Blood, April 15, 2006; 107(8): 3342 - 3349.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Ganguly, M. S. Goel, T. Krasik, K. Bdeir, S. L. Diamond, D. B. Cines, V. R. Muzykantov, and J.-C. Murciano
Fibrin Affinity of Erythrocyte-Coupled Tissue-Type Plasminogen Activators Endures Hemodynamic Forces and Enhances Fibrinolysis in Vivo
J. Pharmacol. Exp. Ther., March 1, 2006; 316(3): 1130 - 1136.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
I. Prudovsky, A. Mandinova, R. Soldi, C. Bagala, I. Graziani, M. Landriscina, F. Tarantini, M. Duarte, S. Bellum, H. Doherty, et al.
The non-classical export routes: FGF1 and IL-1{alpha} point the way
J. Cell Sci., December 15, 2003; 116(24): 4871 - 4881.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003