spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 27 May 2003
doi: 10.1242/jcs.00480

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00480v1
116/14/2929    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morel, A.-P.
Right arrow Articles by Corbo, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morel, A.-P.
Right arrow Articles by Corbo, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

BTG2 antiproliferative protein interacts with the human CCR4 complex existing in vivo in three cell-cycle-regulated forms


Anne-Pierre Morel, Stéphanie Sentis, Claire Bianchin, Muriel Le Romancer, Laurence Jonard, Marie-Claude Rostan, Ruth Rimokh, and Laura Corbo*
* Author for correspondence (e-mail: corbo{at}lyon.fnclcc.fr)

The yeast CCR4-NOT complex exists in two forms (1.0 and 1.9 MDa) that share several common subunits, including yCCR4, yCAF1 and five NOT proteins (NOT1-5). Here, we report that different complexes containing mammalian homologs of CCR4-NOT subunits exist in mammalian cells, with estimated sizes of ~1.9 MDa, ~1 MDa and ~650 kDa, and that BTG2, a member of a protein family with antiproliferative functions, can associate with these complexes. Immunoprecipitation and gel filtration experiments established that BTG2 interacts in vivo with hCCR4 protein via hCAF1 and hPOP2. Moreover, we show that hCCR4, as well as hCAF1 and BTG2, modulate the transcription regulation mediated by ER{alpha}. Finally, we demonstrate that the cellular localization of hCAF1 and the cell content in hCAF1-containing complexes change as cells progress from quiescence to S phase. These findings suggest that the different regulatory pathways in which hCAF1 is involved, notably transcription regulation and mRNA turnover, may occur through distinct CCR4 complexes in the course of cell-cycle progression.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
M. Govindan, X. Meng, C. L. Denis, P. Webb, J. D. Baxter, and P. G. Walfish
Identification of CCR4 and other essential thyroid hormone receptor co-activators by modified yeast synthetic genetic array analysis
PNAS, November 24, 2009; 106(47): 19854 - 19859.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. Aslam, S. Mittal, F. Koch, J.-C. Andrau, and G. S. Winkler
The Ccr4-Not Deadenylase Subunits CNOT7 and CNOT8 Have Overlapping Roles and Modulate Cell Proliferation
Mol. Biol. Cell, September 1, 2009; 20(17): 3840 - 3850.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Yang, M. Morita, H. Wang, T. Suzuki, W. Yang, Y. Luo, C. Zhao, Y. Yu, M. Bartlam, T. Yamamoto, et al.
Crystal structures of human BTG2 and mouse TIS21 involved in suppression of CAF1 deadenylase activity
Nucleic Acids Res., December 1, 2008; 36(21): 6872 - 6881.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Garapaty, M. A. Mahajan, and H. H. Samuels
Components of the CCR4-NOT Complex Function as Nuclear Hormone Receptor Coactivators via Association with the NRC-interacting Factor NIF-1
J. Biol. Chem., March 14, 2008; 283(11): 6806 - 6816.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Ezzeddine, T.-C. Chang, W. Zhu, A. Yamashita, C.-Y. A. Chen, Z. Zhong, Y. Yamashita, D. Zheng, and A.-B. Shyu
Human TOB, an Antiproliferative Transcription Factor, Is a Poly(A)-Binding Protein-Dependent Positive Regulator of Cytoplasmic mRNA Deadenylation
Mol. Cell. Biol., November 15, 2007; 27(22): 7791 - 7801.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Y. Robin-Lespinasse, S. Sentis, C. Kolytcheff, M.-C. Rostan, L. Corbo, and M. Le Romancer
hCAF1, a new regulator of PRMT1-dependent arginine methylation
J. Cell Sci., February 15, 2007; 120(4): 638 - 647.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
L. J. Donato, J. H. Suh, and N. Noy
Suppression of Mammary Carcinoma Cell Growth by Retinoic Acid: the Cell Cycle Control Gene Btg2 Is a Direct Target for Retinoic Acid Receptor Signaling
Cancer Res., January 15, 2007; 67(2): 609 - 615.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. R. DeBella, A. Hayashi, and L. S. Rose
LET-711, the Caenorhabditis elegans NOT1 Ortholog, Is Required for Spindle Positioning and Regulation of Microtubule Length in Embryos
Mol. Biol. Cell, November 1, 2006; 17(11): 4911 - 4924.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. Sentis, M. Le Romancer, C. Bianchin, M.-C. Rostan, and L. Corbo
Sumoylation of the Estrogen Receptor {alpha} Hinge Region Regulates Its Transcriptional Activity
Mol. Endocrinol., November 1, 2005; 19(11): 2671 - 2684.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
C. BIANCHIN, F. MAUXION, S. SENTIS, B. SERAPHIN, and L. CORBO
Conservation of the deadenylase activity of proteins of the Caf1 family in human
RNA, April 1, 2005; 11(4): 487 - 494.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Goldschneider, K. Million, A. Meiller, H. Haddada, A. Puisieux, J. Benard, E. May, and S. Douc-Rasy
The neurogene BTG2TIS21/PC3 is transactivated by {Delta}Np73{alpha} via p53 specifically in neuroblastoma cells
J. Cell Sci., March 15, 2005; 118(6): 1245 - 1253.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Park, Y. J. Lee, H.-J. Lee, T. Seki, K.-H. Hong, J. Park, H. Beppu, I. K. Lim, J.-W. Yoon, E. Li, et al.
B-Cell Translocation Gene 2 (Btg2) Regulates Vertebral Patterning by Modulating Bone Morphogenetic Protein/Smad Signaling
Mol. Cell. Biol., December 1, 2004; 24(23): 10256 - 10262.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Berthet, A.-M. Morera, M.-J. Asensio, M.-A. Chauvin, A.-P. Morel, F. Dijoud, J.-P. Magaud, P. Durand, and J.-P. Rouault
CCR4-Associated Factor CAF1 Is an Essential Factor for Spermatogenesis
Mol. Cell. Biol., July 1, 2004; 24(13): 5808 - 5820.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. G. M. Zwartjes, S. Jayne, D. L. C. van den Berg, and H. T. M. Timmers
Repression of Promoter Activity by CNOT2, a Subunit of the Transcription Regulatory Ccr4-Not Complex
J. Biol. Chem., March 19, 2004; 279(12): 10848 - 10854.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003