spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 2 Jul 2003
doi: 10.1242/jcs.00635

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00635v1
116/16/3327    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayakawa, T.
Right arrow Articles by Hiraoka, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayakawa, T.
Right arrow Articles by Hiraoka, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Cell cycle behavior of human HP1 subtypes: distinct molecular domains of HP1 are required for their centromeric localization during interphase and metaphase


Tomohiro Hayakawa, Tokuko Haraguchi, Hiroshi Masumoto, and Yasushi Hiraoka*
* Author for correspondence (e-mail: yasushi{at}crl.go.jp)

Heterochromatin protein 1 (HP1) plays an important role in heterochromatin formation. Three subtypes of HP1, namely HP1{alpha}, {beta}, and {gamma}, have been identified in humans. In this study, using yellow fluorescent protein (YFP) fusion constructs, we examined the intracellular localization of human HP1 subtypes during the cell cycle. During interphase, all three HP1 subtypes were localized to centromeric heterochromatin and to promyelocytic leukemia (PML) nuclear bodies. Different preferences, however, were observed among the subtypes: during interphase HP1{beta} localized most preferentially to centromeric heterochromatin, whereas HP1{alpha} and {gamma} were more preferentially localized to PML nuclear bodies. During metaphase, only HP1{alpha}, was localized to the centromere. We thus determined which molecular domains of HP1 were necessary for their intracellular localization. Our results showed that the C-terminal fragment (amino acid residues 101-180) of HP1{alpha} was necessary for localization to the metaphase centromere and the N-terminal fragment (amino acid residues 1-76) of HP1{beta} was necessary for localization to the interphase centromere. Interestingly, simultaneous observations of residues 101-180 of HP1{alpha} and residues 1-76 of HP1{beta} in living HeLa cells revealed that during late prophase, the HP1{beta} fragment dissociated from centromeric regions and the HP1{alpha} fragment accumulated in centromeric regions. These results indicate that different specific regions of human HP1{alpha} and HP1{beta} mediate localization to metaphase and interphase centromeric regions resulting in association of different subtypes of HP1 with the centromere at different times during the cell cycle.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
M. Sadaie, R. Kawaguchi, Y. Ohtani, F. Arisaka, K. Tanaka, K. Shirahige, and J.-i. Nakayama
Balance between Distinct HP1 Family Proteins Controls Heterochromatin Assembly in Fission Yeast
Mol. Cell. Biol., December 1, 2008; 28(23): 6973 - 6988.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J. Font-Burgada, D. Rossell, H. Auer, and F. Azorin
Drosophila HP1c isoform interacts with the zinc-finger proteins WOC and Relative-of-WOC to regulate gene expression
Genes & Dev., November 1, 2008; 22(21): 3007 - 3023.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
E. Bartova, J. Krejci, A. Harnicarova, G. Galiova, and S. Kozubek
Histone Modifications and Nuclear Architecture: A Review
J. Histochem. Cytochem., August 1, 2008; 56(8): 711 - 721.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
C.-R. Sihn, Y.-S. Lee, J.-S. Jeong, K. Park, and S. H. Kim
CANu1, a novel nucleolar protein, accumulated on centromere in response to DNA damage
Genes Cells, August 1, 2008; 13(8): 787 - 796.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
R. Zaccarini, F. P. Cordelieres, P. Martin, and S. Saule
PAX6 P46 Binds Chromosomes in the Pericentromeric Region and Induces a Mitosis Defect When Overexpressed
Invest. Ophthalmol. Vis. Sci., December 1, 2007; 48(12): 5408 - 5419.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Sourvinos, N. Tavalai, A. Berndt, D. A. Spandidos, and T. Stamminger
Recruitment of Human Cytomegalovirus Immediate-Early 2 Protein onto Parental Viral Genomes in Association with ND10 in Live-Infected Cells
J. Virol., September 15, 2007; 81(18): 10123 - 10136.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Agarwal, T. Hardt, A. Brero, D. Nowak, U. Rothbauer, A. Becker, H. Leonhardt, and M. C. Cardoso
MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation
Nucleic Acids Res., August 13, 2007; (2007) gkm599v1.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. J. Vogel, L. Guelen, E. de Wit, D. P. Hupkes, M. Loden, W. Talhout, M. Feenstra, B. Abbas, A.-K. Classen, and B. van Steensel
Human heterochromatin proteins form large domains containing KRAB-ZNF genes
Genome Res., December 1, 2006; 16(12): 1493 - 1504.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. E. Norwood, T. J. Moss, N. V. Margaryan, S. L. Cook, L. Wright, E. A. Seftor, M. J. C. Hendrix, D. A. Kirschmann, and L. L. Wallrath
A Requirement for Dimerization of HP1Hs{alpha} in Suppression of Breast Cancer Invasion
J. Biol. Chem., July 7, 2006; 281(27): 18668 - 18676.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H. Nakashima, M. Nakano, R. Ohnishi, Y. Hiraoka, Y. Kaneda, A. Sugino, and H. Masumoto
Assembly of additional heterochromatin distinct from centromere-kinetochore chromatin is required for de novo formation of human artificial chromosome
J. Cell Sci., December 15, 2005; 118(24): 5885 - 5898.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
E. Bartova, J. Pachernik, A. Harnicarova, A. Kovarik, M. Kovarikova, J. Hofmanova, M. Skalnikova, M. Kozubek, and S. Kozubek
Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases
J. Cell Sci., November 1, 2005; 118(21): 5035 - 5046.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Uchiyama, S. Kobayashi, H. Takata, T. Ishihara, N. Hori, T. Higashi, K. Hayashihara, T. Sone, D. Higo, T. Nirasawa, et al.
Proteome Analysis of Human Metaphase Chromosomes
J. Biol. Chem., April 29, 2005; 280(17): 16994 - 17004.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
E. A. Shestakova, Z. Mansuroglu, H. Mokrani, N. Ghinea, and E. Bonnefoy
Transcription factor YY1 associates with pericentromeric {gamma}-satellite DNA in cycling but not in quiescent (G0) cells
Nucleic Acids Res., August 17, 2004; 32(14): 4390 - 4399.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
L. Schmiedeberg, K. Weisshart, S. Diekmann, G. Meyer zu Hoerste, and P. Hemmerich
High- and Low-mobility Populations of HP1 in Heterochromatin of Mammalian Cells
Mol. Biol. Cell, June 1, 2004; 15(6): 2819 - 2833.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. H. Eskiw, G. Dellaire, and D. P. Bazett-Jones
Chromatin Contributes to Structural Integrity of Promyelocytic Leukemia Bodies through a SUMO-1-independent Mechanism
J. Biol. Chem., March 5, 2004; 279(10): 9577 - 9585.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003