spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 22 Jul 2003
doi: 10.1242/jcs.00664

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00664v1
116/17/3645    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chatterjee, S.
Right arrow Articles by Shah, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chatterjee, S.
Right arrow Articles by Shah, V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Inhibition of GTP-dependent vesicle trafficking impairs internalization of plasmalemmal eNOS and cellular nitric oxide production


Suvro Chatterjee, Sheng Cao, Timothy E. Peterson, Robert D. Simari, and Vijay Shah*
* Author for correspondence (e-mail: shah.vijay{at}mayo.edu)

The Ca2+ mobilizing peptide, bradykinin (BK), stimulates endothelial nitric oxide synthase (eNOS)-derived cellular nitric oxide (NO) production in association with altering the subcellular distribution of the enzyme. In the present study we examine the influence of cellular GTPases, particularly the large GTPase dynamin, on BK-mediated eNOS localization and cellular NO production. BK stimulation of ECV cells, which were stably transfected with eNOS-GFP (eNOS-GFP ECV304), increased NO production. This was associated with the mobilization of eNOS-GFP protein into Triton X-100-insoluble fractions of cell lysates, and an internalization of plasmalemmal eNOS-GFP in live and fixed ECV 304 cells. Incubation of digitonin-permeabilized ECV304 cells with the non-hydrolyzed GTP analog, GTP-{gamma}-S, abrogated the BK-mediated internalization of eNOS-GFP as assessed by confocal microscopy. Conversely, inhibition of clathrin-dependent endocytosis, via overexpression of AP 180 or pretreatment of cells with chlorpromazine, did not influence BK-mediated eNOS redistribution. Furthermore, specific inhibition of dynamin-2 GTPase function by overexpression of a dominant negative construct, K44A, prevented the BK-mediated enrichment of eNOS-GFP within low buoyant density, caveolin-enriched fractions of eNOS-GFP ECV304 cell lysates. Dynamin-2 K44A overexpression also markedly impaired BK-dependent, L-NAME-inhibited NO production as did incubation of permeabilized cells with GTP-{gamma}-s. These studies demonstrate that disruption of dynamin- and GTP-dependent, but clathrin-independent, vesicle trafficking pathways impairs BK-dependent cellular NO production, via inhibition of the internalization of eNOS-containing plasmalemmal vesicles.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Cell Physiol.Home page
P. N. Bernatchez, A. Sharma, P. Kodaman, and W. C. Sessa
Myoferlin is critical for endocytosis in endothelial cells
Am J Physiol Cell Physiol, January 1, 2009; 297(3): C484 - C492.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
F. A. Sanchez, D. D. Kim, R. G. Duran, C. J. Meininger, and W. N. Duran
Internalization of eNOS via caveolae regulates PAF-induced inflammatory hyperpermeability to macromolecules
Am J Physiol Heart Circ Physiol, October 1, 2008; 295(4): H1642 - H1648.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Kang-Decker, S. Cao, S. Chatterjee, J. Yao, L. J. Egan, D. Semela, D. Mukhopadhyay, and V. Shah
Nitric oxide promotes endothelial cell survival signaling through S-nitrosylation and activation of dynamin-2
J. Cell Sci., February 1, 2007; 120(3): 492 - 501.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
N. A. Maniatis, V. Brovkovych, S. E. Allen, T. A. John, A. N. Shajahan, C. Tiruppathi, S. M. Vogel, R. A. Skidgel, A. B. Malik, and R. D. Minshall
Novel Mechanism of Endothelial Nitric Oxide Synthase Activation Mediated by Caveolae Internalization in Endothelial Cells
Circ. Res., October 13, 2006; 99(8): 870 - 877.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Mukherjee, M. Tessema, and A. Wandinger-Ness
Vesicular Trafficking of Tyrosine Kinase Receptors and Associated Proteins in the Regulation of Signaling and Vascular Function
Circ. Res., March 31, 2006; 98(6): 743 - 756.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. Icking, S. Matt, N. Opitz, A. Wiesenthal, W. Muller-Esterl, and K. Schilling
NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS
J. Cell Sci., November 1, 2005; 118(21): 5059 - 5069.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Schleicher, F. Brundin, S. Gross, W. Muller-Esterl, and S. Oess
Cell Cycle-Regulated Inactivation of Endothelial NO Synthase through NOSIP-Dependent Targeting to the Cytoskeleton
Mol. Cell. Biol., September 15, 2005; 25(18): 8251 - 8258.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. S. Lee, N. Kang Decker, S. Chatterjee, J. Yao, S. Friedman, and V. Shah
Mechanisms of Nitric Oxide Interplay with Rho GTPase Family Members in Modulation of Actin Membrane Dynamics in Pericytes and Fibroblasts
Am. J. Pathol., June 1, 2005; 166(6): 1861 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
I. Lim, S. J Gibbons, G. L. Lyford, S. M. Miller, P. R. Strege, M. G. Sarr, S. Chatterjee, J. H. Szurszewski, V. H. Shah, and G. Farrugia
Carbon monoxide activates human intestinal smooth muscle L-type Ca2+ channels through a nitric oxide-dependent mechanism
Am J Physiol Gastrointest Liver Physiol, January 1, 2005; 288(1): G7 - G14.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J.-P. Gratton, P. Bernatchez, and W. C. Sessa
Caveolae and Caveolins in the Cardiovascular System
Circ. Res., June 11, 2004; 94(11): 1408 - 1417.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003