spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 2 Sep 2003
doi: 10.1242/jcs.00715

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00715v1
116/20/4249    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vlieghe, K.
Right arrow Articles by De Veylder, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vlieghe, K.
Right arrow Articles by De Veylder, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Microarray analysis of E2Fa-DPa-overexpressing plants uncovers a cross-talking genetic network between DNA replication and nitrogen assimilation


Kobe Vlieghe, Marnik Vuylsteke, Kobe Florquin, Stephane Rombauts, Sara Maes, Sandra Ormenese, Paul Van Hummelen, Yves Van de Peer, Dirk Inzé*, and Lieven De Veylder
* Author for correspondence (e-mail: dirk.inze{at}psb.ugent.be)

Previously we have shown that overexpression of the heterodimeric E2Fa-DPa transcription factor in Arabidopsis thaliana results in ectopic cell division, increased endoreduplication, and an early arrest in development. To gain a better insight into the phenotypic behavior of E2Fa-DPa transgenic plants and to identify E2Fa-DPa target genes, a transcriptomic microarray analysis was performed. Out of 4,390 unique genes, a total of 188 had a twofold or more up- (84) or down-regulated (104) expression level in E2Fa-DPa transgenic plants compared to wild-type lines. Detailed promoter analysis allowed the identification of novel E2Fa-DPa target genes, mainly involved in DNA replication. Secondarily induced genes encoded proteins involved in cell wall biosynthesis, transcription and signal transduction or had an unknown function. A large number of metabolic genes were modified as well, among which, surprisingly, many genes were involved in nitrate assimilation. Our data suggest that the growth arrest observed upon E2Fa-DPa overexpression results at least partly from a nitrogen drain to the nucleotide synthesis pathway, causing decreased synthesis of other nitrogen compounds, such as amino acids and storage proteins.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. T. Ascencio-Ibanez, R. Sozzani, T.-J. Lee, T.-M. Chu, R. D. Wolfinger, R. Cella, and L. Hanley-Bowdoin
Global Analysis of Arabidopsis Gene Expression Uncovers a Complex Array of Changes Impacting Pathogen Response and Cell Cycle during Geminivirus Infection
Plant Physiology, September 1, 2008; 148(1): 436 - 454.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Ramirez-Parra and C. Gutierrez
E2F Regulates FASCIATA1, a Chromatin Assembly Gene Whose Loss Switches on the Endocycle and Activates Gene Expression by Changing the Epigenetic Status
Plant Physiology, May 1, 2007; 144(1): 105 - 120.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. C. del Pozo, S. Diaz-Trivino, N. Cisneros, and C. Gutierrez
The Balance between Cell Division and Endoreplication Depends on E2FC-DPB, Transcription Factors Regulated by the Ubiquitin-SCFSKP2A Pathway in Arabidopsis
PLANT CELL, September 1, 2006; 18(9): 2224 - 2235.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Desvoyes, E. Ramirez-Parra, Q. Xie, N.-H. Chua, and C. Gutierrez
Cell Type-Specific Role of the Retinoblastoma/E2F Pathway during Arabidopsis Leaf Development
Plant Physiology, January 1, 2006; 140(1): 67 - 80.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Vandepoele, K. Vlieghe, K. Florquin, L. Hennig, G. T.S. Beemster, W. Gruissem, Y. Van de Peer, D. Inze, and L. De Veylder
Genome-Wide Identification of Potential Plant E2F Target Genes
Plant Physiology, September 1, 2005; 139(1): 316 - 328.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Corellou, A. Camasses, L. Ligat, G. Peaucellier, and F.-Y. Bouget
Atypical Regulation of a Green Lineage-Specific B-Type Cyclin-Dependent Kinase
Plant Physiology, July 1, 2005; 138(3): 1627 - 1636.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
V. Boudolf, K. Vlieghe, G. T.S. Beemster, Z. Magyar, J. A. T. Acosta, S. Maes, E. Van Der Schueren, D. Inze, and L. De Veylder
The Plant-Specific Cyclin-Dependent Kinase CDKB1;1 and Transcription Factor E2Fa-DPa Control the Balance of Mitotically Dividing and Endoreduplicating Cells in Arabidopsis
PLANT CELL, October 1, 2004; 16(10): 2683 - 2692.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Ramirez-Parra, M. A. Lopez-Matas, C. Frundt, and C. Gutierrez
Role of an Atypical E2F Transcription Factor in the Control of Arabidopsis Cell Growth and Differentiation
PLANT CELL, September 1, 2004; 16(9): 2350 - 2363.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003