spacer gif spacer gif spacer gif spacer gif Propose a workshop for 2011 spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 19 Nov 2003
doi: 10.1242/jcs.00828

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.00828v1
117/1/73    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Casar, J. C.
Right arrow Articles by Brandan, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casar, J. C.
Right arrow Articles by Brandan, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Heparan sulfate proteoglycans are increased during skeletal muscle regeneration: requirement of syndecan-3 for successful fiber formation


Juan Carlos Casar, Claudio Cabello-Verrugio, Hugo Olguin, Rebeca Aldunate, Nibaldo C. Inestrosa, and Enrique Brandan*
* Author for correspondence (e-mail: ebrandan{at}genes.bio.puc.cl)

Skeletal muscle regeneration is a highly complex and regulated process that involves muscle precursor proliferation and differentiation and probably requires the participation of heparin binding growth factors such as FGFs, HGF and TGF{beta}. Heparan sulfate proteoglycans, key components of cell-surfaces and ECM, modulate growth factor activities and influence cell growth and differentiation. Their expression in forming muscle masses during development and in cell culture, suggest their participation in the regulation of myogenesis. In the present study, heparan sulfate proteoglycan expression in skeletal muscle regeneration induced by barium chloride injection was evaluated. Expression of muscle differentiation markers and neuromuscular junction (NMJ) components was characterized. Immunoblots with anti-{Delta}-heparan sulfate antibody showed that four major species - perlecan, glypican, syndecan-3 and syndecan-4 - were transiently up-regulated. The first three were detected at the surface or basement membranes of newly formed myotubes by specific indirect immunofluorescence. Syndecan-3, a satellite cell marker, showed the earliest and most significant increase. Experiments involving myoblast grafting into regenerating muscle showed that C2C12 cell clones, with inhibited syndecan-3 expression resulting from antisense transfection, presented a normal proliferation rate but an impaired capacity to fuse and form skeletal muscle fibers. These data constitute the first in vivo evidence suggesting the requirement of a specific heparan sulfate proteoglycan for successful skeletal muscle regeneration.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 In VivoHome page
M. KARALAKI, S. FILI, A. PHILIPPOU, and M. KOUTSILIERIS
Muscle Regeneration: Cellular and Molecular Events
In Vivo, September 1, 2009; 23(5): 779 - 796.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
L. H. Jorgensen, S. J. Petersson, J. Sellathurai, D. C. Andersen, S. Thayssen, D. J. Sant, C. H. Jensen, and H. D. Schroder
Secreted Protein Acidic and Rich in Cysteine (SPARC) in Human Skeletal Muscle
J. Histochem. Cytochem., January 1, 2009; 57(1): 29 - 39.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
P. Sasse, D. Malan, M. Fleischmann, W. Roell, E. Gustafsson, T. Bostani, Y. Fan, T. Kolbe, M. Breitbach, K. Addicks, et al.
Perlecan is critical for heart stability
Cardiovasc Res, December 1, 2008; 80(3): 435 - 444.
[Abstract] [Full Text] [PDF]

Home page
 Ann Rheum DisHome page
A M Patterson, A Cartwright, G David, O Fitzgerald, B Bresnihan, B A Ashton, and J Middleton
Differential expression of syndecans and glypicans in chronically inflamed synovium
Ann Rheum Dis, May 1, 2008; 67(5): 592 - 601.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
C. A. C. Ottenheijm, G. J. Jenniskens, M. C. P. Geraedts, T. Hafmans, L. M. A. Heunks, T. H. van Kuppevelt, and P. N. R. Dekhuijzen
Diaphragm dysfunction in chronic obstructive pulmonary disease: a role for heparan sulphate?
Eur. Respir. J., July 1, 2007; 30(1): 80 - 89.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. A. Timmons, O. Larsson, E. Jansson, H. Fischer, T. Gustafsson, P. L. Greenhaff, J. Ridden, J. Rachman, M. Peyrard-Janvid, C. Wahlestedt, et al.
Human muscle gene expression responses to endurance training provide a novel perspective on Duchenne muscular dystrophy
FASEB J, May 1, 2005; 19(7): 750 - 760.
[Abstract] [Full Text] [PDF]

Home page
 Am J Sports MedHome page
T. A. H. Jarvinen, T. L. N. Jarvinen, M. Kaariainen, H. Kalimo, and M. Jarvinen
Muscle Injuries: Biology and Treatment
Am. J. Sports Med., May 1, 2005; 33(5): 745 - 764.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Tkachenko, J. M. Rhodes, and M. Simons
Syndecans: New Kids on the Signaling Block
Circ. Res., March 18, 2005; 96(5): 488 - 500.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
R. Araya, D. Eckardt, S. Maxeiner, O. Kruger, M. Theis, K. Willecke, and J. C. Saez
Expression of connexins during differentiation and regeneration of skeletal muscle: functional relevance of connexin43
J. Cell Sci., January 1, 2005; 118(1): 27 - 37.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
A. N. Minniti, M. Labarca, C. Hurtado, and E. Brandan
Caenorhabditis elegans syndecan (SDN-1) is required for normal egg laying and associates with the nervous system and the vulva
J. Cell Sci., October 1, 2004; 117(21): 5179 - 5190.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2003