spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 31 Jul 2007
doi: 10.1242/jcs.010181

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.010181v1
120/16/2895    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baur, T.
Right arrow Articles by Meyer, H. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baur, T.
Right arrow Articles by Meyer, H. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

NSF- and SNARE-mediated membrane fusion is required for nuclear envelope formation and completion of nuclear pore complex assembly in Xenopus laevis egg extracts


Tina Baur, Kristijan Ramadan, Andreas Schlundt, Jürgen Kartenbeck, and Hemmo H. Meyer*
* Author for correspondence (e-mail: hemmo.meyer{at}bc.biol.ethz.ch)

Despite the progress in understanding nuclear envelope (NE) reformation after mitosis, it has remained unclear what drives the required membrane fusion and how exactly this is coordinated with nuclear pore complex (NPC) assembly. Here, we show that, like other intracellular fusion reactions, NE fusion in Xenopus laevis egg extracts is mediated by SNARE proteins that require activation by NSF. Antibodies against Xenopus NSF, depletion of NSF or the dominant-negative NSFE329Q variant specifically inhibited NE formation. Staging experiments further revealed that NSF was required until sealing of the envelope was completed. Moreover, excess exogenous {alpha}-SNAP that blocks SNARE function prevented membrane fusion and caused accumulation of non-flattened vesicles on the chromatin surface. Under these conditions, the nucleoporins Nup107 and gp210 were fully recruited, whereas assembly of FxFG-repeat-containing nucleoporins was blocked. Together, we define NSF- and SNARE-mediated membrane fusion events as essential steps during NE formation downstream of Nup107 recruitment, and upstream of membrane flattening and completion of NPC assembly.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. R. Rafikova, K. Melikov, C. Ramos, L. Dye, and L. V. Chernomordik
Transmembrane Protein-free Membranes Fuse into Xenopus Nuclear Envelope and Promote Assembly of Functional Pores
J. Biol. Chem., October 23, 2009; 284(43): 29847 - 29859.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. K. Lau, V. A. Delmar, R. C. Chan, Q. Phung, C. Bernis, B. Fichtman, B. A. Rasala, and D. J. Forbes
Transportin Regulates Major Mitotic Assembly Events: From Spindle to Nuclear Pore Assembly
Mol. Biol. Cell, September 15, 2009; 20(18): 4043 - 4058.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. M. Tartakoff and P. Jaiswal
Nuclear Fusion and Genome Encounter during Yeast Zygote Formation
Mol. Biol. Cell, June 15, 2009; 20(12): 2932 - 2942.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
S. Mukhopadhyay, J. Lee, and P. B. Sehgal
Depletion of the ATPase NSF from Golgi membranes with hypo-S-nitrosylation of vasorelevant proteins in endothelial cells exposed to monocrotaline pyrrole
Am J Physiol Heart Circ Physiol, November 1, 2008; 295(5): H1943 - H1955.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
B. A. Rasala, C. Ramos, A. Harel, and D. J. Forbes
Capture of AT-rich Chromatin by ELYS Recruits POM121 and NDC1 to Initiate Nuclear Pore Assembly
Mol. Biol. Cell, September 1, 2008; 19(9): 3982 - 3996.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. J. Anderson and M. W. Hetzer
Shaping the endoplasmic reticulum into the nuclear envelope
J. Cell Sci., January 15, 2008; 121(2): 137 - 142.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2007