spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search    

The fully linked HTML version of this article has now been published.
JCS ePress online publication date 23 Mar 2004
doi: 10.1242/jcs.01044

This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
jcs.01044v1
117/10/1971    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, X.
Right arrow Articles by Walker, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, X.
Right arrow Articles by Walker, J. W.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Research Article

Myofilament anchoring of protein kinase C-epsilon in cardiac myocytes


Xupei Huang* and Jeffery W. Walker
* Author for correspondence (e-mail: xhuang{at}fau.edu)

Regulatory proteins on muscle filaments are substrates for protein kinase C (PKC) but mechanisms underlying activation and translocation of PKC to this non-membrane compartment are poorly understood. Here we demonstrate that the epsilon isoform of PKC ({epsilon}-PKC) activated by arachidonic acid (AA) binds reversibly to cardiac myofibrils with an EC50 of 86 nM. Binding occurred near the Z-lines giving rise to a striated staining pattern. The delta isoform of PKC ({delta}-PKC) did not bind to cardiac myofibrils regardless of the activator used, and the alpha isoform ({alpha}-PKC) bound only under strong activating conditions. Three established PKC anchoring proteins, filamentous actin (F-actin), the LIM domain protein Cypher-1, and the coatamer protein {beta}'-COP were each tested for their involvement in cytoskeletal anchoring. F-actin bound {epsilon}-PKC selectively over {delta}-PKC and {alpha}-PKC, but this interaction was readily distinguishable from cardiac myofilament binding in two ways. First, the F-actin/{epsilon}-PKC interaction was independent of PKC activation, and second, the synthetic hexapeptide LKKQET derived from the C1 region of {epsilon}-PKC effectively blocked {epsilon}-PKC binding to F-actin, but was without effect on its binding to cardiac myofilaments. Involvement of Cypher-1 was ruled out on the basis of its absence from detergent-skinned myofibrils that bound {epsilon}-PKC, despite its presence in intact cardiac myocytes. The {epsilon}-PKC translocation inhibitor peptide EAVSLKPT reduced activated {epsilon}-PKC binding to cardiac myofibrils in a concentration dependent manner, suggesting that a RACK2 or a similar protein plays a role in {epsilon}-PKC anchoring in cardiac myofilaments.
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
R. A. Fenton, S. Komatsu, M. Ikebe, L. G. Shea, and J. G. Dobson Jr.
Adenoprotection of the heart involves phospholipase C-induced activation and translocation of PKC-{varepsilon} to RACK2 in adult rat and mouse
Am J Physiol Heart Circ Physiol, August 1, 2009; 297(2): H718 - H725.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Molnar, A. Borbely, D. Czuriga, S. M. Ivetta, S. Szilagyi, Z. Hertelendi, E. T. Pasztor, A. Balogh, Z. Galajda, T. Szerafin, et al.
Protein Kinase C Contributes to the Maintenance of Contractile Force in Human Ventricular Cardiomyocytes
J. Biol. Chem., January 9, 2009; 284(2): 1031 - 1039.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
R. J. Belin, M. P. Sumandea, E. J. Allen, K. Schoenfelt, H. Wang, R. J. Solaro, and P. P. de Tombe
Augmented Protein Kinase C-{alpha}-Induced Myofilament Protein Phosphorylation Contributes to Myofilament Dysfunction in Experimental Congestive Heart Failure
Circ. Res., July 20, 2007; 101(2): 195 - 204.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
Y. Li, J. M. Urban, M. L. Cayer, H. K. Plummer III, and C. A. Heckman
Actin-based features negatively regulated by protein kinase C-{epsilon}
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1002 - C1013.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. M. Samarel
Costameres, focal adhesions, and cardiomyocyte mechanotransduction
Am J Physiol Heart Circ Physiol, December 1, 2005; 289(6): H2291 - H2301.
[Abstract] [Full Text] [PDF]


© The Company of Biologists Ltd 2004